The modification of electrodes is always an important task in electrochemical detection of electroactive and biological molecules. Chemically modified electrodes can offer improved selectivity and sensitivity for the target analyte, which greatly enhance the electrode performance. Various materials such as conducting polymers, metal nanoparticles and carbon nanomaterials have been exploited and widely used for the modification of electrodes. Electrochemical or spontaneous deposition, electrostatic adsorption, layer-by-layer self assembly and covalent binding have also been developed for electrode modification and offer improved performance. Both Prussian blue (PB) and toluidine blue O (TBO) are excellent redox mediators and very popular in electrode modification. PB has shown strong catalytic property for the reduction of hydrogen peroxide, but the application in biosensor fabrication is limited for its instability at neutral pH. Graphene, as a single-atom-thick carbon material, is considered an ideal platform for designing composite nanomaterials for high-performance electrochemical or electrocatalytic devices. The combination of PB with reduced graphene oxide (RGO) and poly(toluidine blue O) (PTBO) will greatly improve the stability of PB. An amperometric biosensor based on glassy carbon (GC) electrode modified with reduced graphene oxide, PB and poly(toluidine blue O) was developed. Experimental results showed that the GC/RGO/PB/PTBO modified electrode offered an excellent electrocatalytic activity toward the reduction of hydrogen peroxide due to the possible synergistic effects of the PB-PTBO composite material. After codeposition of glucose oxidase (GOD) and chitosan (CHIT) coating, the resulting GC/RGO/PB/ PTBO/CHIT-GOD electrode exhibited excellent response to glucose with a sensitivity of 59 mA M1 cm2, a low detection limit of 8.4 μM and a linear range from 0.02 to 1.09 mM at a detection potential of +0.2 V vs. Ag.
|Date of Award||12 Nov 2014|
|Supervisor||Kwok Keung SHIU (Supervisor)|
- Electrochemical sensors
- Nanocomposites (Materials)