Coordination of division timing for intestine precursor cells during C.elegans embryogenesis

  • Ming Kin Wong

Student thesis: Master's Thesis

Abstract

Metazoan development is a complex and tightly controlled process that not only requires precise cell fate differentiation, but also demands accurate timing of cell division and precise cell migration. Genetic regulation of cell cycle length throughout metazoans embryogenesis is largely unknown, mainly due to the technical hurdle in quantifying cell division timing during development. Caenorhabditis elegans embryogenesis provides an excellent opportunity to study the genetic regulation of division timing because of its invariant cell lineage and widespread division asynchronies between sister cells. A combination of in toto imaging and automated cell lineaging coupled with high throughput RNAi allows genetic screening of genes involved in regulation of Asynchrony of Division between Sister cells (ADS) or cousin cells. One of the most pronounced asynchronies between cousin cells during C. elegans embryogenesis is a significant elongation of division timing in two endoderm progenitor cells, Ea and Ep (E2), versus their cousins MSa and MSp (MS2) that mainly develop into mesoderm organs. Out of a total of 822 essential and conserved genes that were perturbed by RNAi in our previous genetic screening, 53 genes are found to produce significantly reduction specifically in the E2 cell cycle length (p
Date of Award5 Aug 2016
Original languageEnglish
SupervisorZhongying ZHAO (Supervisor)

User-Defined Keywords

  • Caenorhabditis elegans
  • Metazoa

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