Wide spectral-range imaging spectroscopy of photonic crystal microbeads for multiplex biomolecular assay applications

Jianping LI*

*Corresponding author for this work

Research output: Chapter in book/report/conference proceedingConference proceedingpeer-review

Abstract

Suspension assay using optically color-encoded microbeads is a novel way to increase the reaction speed and multiplex of biomolecular detection and analysis. To boost the detection speed, a hyperspectral imaging (HSI) system is of great interest for quickly decoding the color codes of the microcarriers. Imaging Fourier transform spectrometer (IFTS) is a potential candidate for this task due to its advantages in HSI measurement. However, conventional IFTS is only popular in IR spectral bands because it is easier to track its scanning mirror position in longer wavelengths so that the fundamental Nyquist criterion can be satisfied when sampling the interferograms; the sampling mechanism for shorter wavelengths IFTS used to be very sophisticated, high-cost and bulky. In order to overcome this handicap and take better usage of its advantages for HSI applications, a new wide spectral range IFTS platform is proposed based on an optical beam-folding position-tracking technique. This simple technique has successfully extended the spectral range of an IFTS to cover 350-1000nm. Test results prove that the system has achieved good spectral and spatial resolving performances with instrumentation flexibilities. Accurate and fast measurement results on novel colloidal photonic crystal microbeads also demonstrate its practical potential for high-throughput and multiplex suspension molecular assays..

Original languageEnglish
Title of host publicationTranslational Biophotonics
PublisherSPIE
ISBN (Print)9780819496263
DOIs
Publication statusPublished - 2014
EventTranslational Biophotonics - Houston, TX, United States
Duration: 19 May 201420 May 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9155
ISSN (Print)1605-7422

Conference

ConferenceTranslational Biophotonics
Country/TerritoryUnited States
CityHouston, TX
Period19/05/1420/05/14

Scopus Subject Areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

User-Defined Keywords

  • high-throughput
  • hyperspectral imaging
  • imaging Fourier transform spectroscopy
  • multiplex
  • photonic crystal bead
  • suspension assay

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