Whole-Teflon microfluidic chips

Kangning REN, Wen Dai, Jianhua Zhou, Jing Su, Hongkai Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

140 Citations (Scopus)

Abstract

Although microfluidics has shown exciting potential, its broad applications are significantly limited by drawbacks of the materials used to make them. In this work, we present a convenient strategy for fabricating whole-Teflon microfluidic chips with integrated valves that show outstanding inertness to various chemicals and extreme resistance against all solvents. Compared with other microfluidic materials [e.g., poly(dimethylsiloxane) (PDMS)] the whole-Teflon chip has a few more advantages, such as no absorption of small molecules, little adsorption of biomolecules onto channel walls, and no leaching of residue molecules from the material bulk into the solution in the channel. Various biological cells have been cultured in the whole-Teflon channel. Adherent cells can attach to the channel bottom, spread, and proliferate well in the channels (with similar proliferation rate to the cells in PDMS channels with the same dimensions). The moderately good gas permeability of the Teflon materials makes it suitable to culture cells inside the microchannels for a long time.

Original languageEnglish
Pages (from-to)8162-8166
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number20
DOIs
Publication statusPublished - 17 May 2011

Scopus Subject Areas

  • General

User-Defined Keywords

  • Microchips
  • On-chip cell culture
  • Solvent resistive chip

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