TY - JOUR
T1 - Waste-to-biofuel
T2 - production of biobutanol from sago waste residues
AU - Johnravindar, Davidraj
AU - Murugesan, Kumarasamy
AU - WONG, Jonathan W C
AU - Elangovan, Namasivayam
N1 - Funding Information:
This work was partially supported by University Grant Commission (UGC), India. [F No. 37- 300/2009 (SR)].
PY - 2017/7/18
Y1 - 2017/7/18
N2 - The main concern of extensive production of biobutanol has been associated with the high cost of the substrate and the relatively low tolerance of Clostridia to biobutanol production. In this study, the use of fermentable cassava waste residue (CWR) as substrate for biobutanol production was investigated using solvent-tolerant Clostridium sp. Four of obligatory, solvent-producing bacteria were isolated from sago industry waste sites. The NSW, PNAS1, SB5 and SBI4 strains showed identical profiles of 16S rRNA gene sequence similarity of Bacillus coagulans, Clostridium bifermentans and Clostridium sp. (97% similarity) and a wide range of carbohydrate substrate; however, the CWR was found to be suitable for the production of biobutanol considerably. Batch culture study was carried out using parameters such as time and temperature and carbon sources have been studied and optimized. Using pre-optimized CWR medium, significant amount of solvent production was observed in NSW, PNAS1, SB5 and SBI4 with 1.53, 3.36, 1.56 and 2.5 g L−1of butanol yield and 6.84, 9.012, 8.32 and 8.22 g L−1of total solvents, respectively. On the basis of these studies, NSW is proposed to represent the B. coagulans for butanol production directly from sago waste residues.
AB - The main concern of extensive production of biobutanol has been associated with the high cost of the substrate and the relatively low tolerance of Clostridia to biobutanol production. In this study, the use of fermentable cassava waste residue (CWR) as substrate for biobutanol production was investigated using solvent-tolerant Clostridium sp. Four of obligatory, solvent-producing bacteria were isolated from sago industry waste sites. The NSW, PNAS1, SB5 and SBI4 strains showed identical profiles of 16S rRNA gene sequence similarity of Bacillus coagulans, Clostridium bifermentans and Clostridium sp. (97% similarity) and a wide range of carbohydrate substrate; however, the CWR was found to be suitable for the production of biobutanol considerably. Batch culture study was carried out using parameters such as time and temperature and carbon sources have been studied and optimized. Using pre-optimized CWR medium, significant amount of solvent production was observed in NSW, PNAS1, SB5 and SBI4 with 1.53, 3.36, 1.56 and 2.5 g L−1of butanol yield and 6.84, 9.012, 8.32 and 8.22 g L−1of total solvents, respectively. On the basis of these studies, NSW is proposed to represent the B. coagulans for butanol production directly from sago waste residues.
KW - ABE (Acetone–Butanol–Ethanol)
KW - Biobutanol
KW - Cassava waste residues
KW - Clostridium sp
UR - http://www.scopus.com/inward/record.url?scp=85013223057&partnerID=8YFLogxK
U2 - 10.1080/09593330.2017.1283362
DO - 10.1080/09593330.2017.1283362
M3 - Journal article
C2 - 28091177
AN - SCOPUS:85013223057
SN - 0959-3330
VL - 38
SP - 1725
EP - 1734
JO - Environmental Technology (United Kingdom)
JF - Environmental Technology (United Kingdom)
IS - 13-14
ER -
