TY - JOUR
T1 - Visualization of Zn2+ ions in live zebrafish using a luminescent iridium(III) chemosensor
AU - Ma, Dik Lung
AU - He, Hong Zhang
AU - Zhong, Hai Jing
AU - Lin, Sheng
AU - Chan, Daniel Shiu Hin
AU - Wang, Liang
AU - Lee, Simon Ming Yuen
AU - Leung, Chung Hang
AU - Wong, Chun Yuen
N1 - Funding information:
This work was supported by Hong Kong Baptist University (HKBU) (Grants FRG2/12-13/021 and FRG2/13-14/008), the Centre for Cancer and Inflammation Research, the School of Chinese Medicine, HKBU (Grant CCIR-SCM), the Health and Medical Research Fund (Grant HMRF/13121482), the Research Grants Council (Grants HKBU/201811, HKBU/204612, HKBU/201913, and CityU 103911), the French National Research Agency/Research Grants Council Joint Research Scheme (Grant A-HKBU201/12), the State Key Laboratory of Environmental and Biological Analysis and Strategic Development Fund of HKBU, the Science and Technology Development Fund, Macau Special Administrative Region (SAR) (Grant 103/2012/A3), and the University of Macau (Grants MYRG091(Y3-L2)-ICMS12-LCH, MYRG121(Y3-L2)-ICMS12-LCH, MRG007/LCH/2014/ICMS, and MRG023/LCH/2013/ICMS).
Publisher copyright:
© 2014 American Chemical Society
PY - 2014/8/27
Y1 - 2014/8/27
N2 - A novel luminescent cyclometalated iridium(III) complex-based chemosensor (1) bearing a zinc-specific receptor, tris(2-pyridylmethyl)amine, and the 3-phenyl-1H-pyrazole ligand has been designed and synthesized. Upon the addition of Zn2+ ions to a solution of iridium(III) complex 1, a pronounced luminescence color change from blue to green can be observed, which may be attributed to the suppression of photoinduced electron transfer upon complexation of complex 1 with Zn2+ ions. The interaction of iridium(III) complex 1 with Zn2+ ions was investigated by UV-vis absorption titration, emission titration, and 1H NMR titration. Furthermore, the iridium(III) complex 1 exhibited good selectivity for Zn 2+ over 13 other common metal ions, including K+, Ag +, Na+, Ni2+, Fe3+, Hg2+, Cd2+, Mg2+, Ca2+, Cu2+, Mn 2+, Co2+, and Pb2+ ions. The practical application of the iridium(III) complex 1 in visualizing intracellular Zn 2+ distribution in live zebrafish was also demonstrated.
AB - A novel luminescent cyclometalated iridium(III) complex-based chemosensor (1) bearing a zinc-specific receptor, tris(2-pyridylmethyl)amine, and the 3-phenyl-1H-pyrazole ligand has been designed and synthesized. Upon the addition of Zn2+ ions to a solution of iridium(III) complex 1, a pronounced luminescence color change from blue to green can be observed, which may be attributed to the suppression of photoinduced electron transfer upon complexation of complex 1 with Zn2+ ions. The interaction of iridium(III) complex 1 with Zn2+ ions was investigated by UV-vis absorption titration, emission titration, and 1H NMR titration. Furthermore, the iridium(III) complex 1 exhibited good selectivity for Zn 2+ over 13 other common metal ions, including K+, Ag +, Na+, Ni2+, Fe3+, Hg2+, Cd2+, Mg2+, Ca2+, Cu2+, Mn 2+, Co2+, and Pb2+ ions. The practical application of the iridium(III) complex 1 in visualizing intracellular Zn 2+ distribution in live zebrafish was also demonstrated.
KW - chemosensor
KW - iridium(III) complex
KW - luminescent
KW - zinc ion
UR - http://www.scopus.com/inward/record.url?scp=84906819168&partnerID=8YFLogxK
U2 - 10.1021/am504369b
DO - 10.1021/am504369b
M3 - Journal article
C2 - 25051997
AN - SCOPUS:84906819168
SN - 1944-8244
VL - 6
SP - 14008
EP - 14015
JO - ACS Applied Materials and Interfaces
JF - ACS Applied Materials and Interfaces
IS - 16
ER -