TY - JOUR
T1 - Visualization of lipids in cottonseeds by matrix-assisted laser desorption/ionization mass spectrometry imaging
AU - Liu, Bingbing
AU - Meng, Xuanlin
AU - Li, Kun
AU - Guo, Jinggong
AU - Cai, Zongwei
N1 - Funding Information:
This work was supported by the National Key Research and Development Program of China (Grant No. 2017YFC1600500 ). The authors thank Prof. Guanghui Xiao ( Shanxi Normal University ) for providing the cottonseeds for the experiment, and thank funding from the 111 Project of China #D16014 .
PY - 2021/1/1
Y1 - 2021/1/1
N2 - The cotton plant is an essential crop cultivated globally for its fiber and seeds. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used to study the spatial distribution patterns of lipids in cottonseeds. 448 lipid ions were identified by LC-MS/MS, and 24 of which were precisely visualized by using MALDI-MSI. The lipids, including phosphatidylcholines (PC), phosphatidylethanolamines (PE) and triacylglycerols (TG) showed heterogeneous distribution patterns within the cotyledonary and radicle tissues. Additionally, the roles these lipids played in the metabolic pathways were analyzed, and relationship of the spatial distribution of LPC (lysophosphatidylcholine) and corresponding PC was studied. The unique distribution patterns of these lipid metabolites revealed by MSI can provide new insights into areas relating to the spatial compartmentation of lipid metabolism in plants. We believe that the results of MSI, if combined with transcriptomics and proteomics, may offer significant help in genetic engineering work.
AB - The cotton plant is an essential crop cultivated globally for its fiber and seeds. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used to study the spatial distribution patterns of lipids in cottonseeds. 448 lipid ions were identified by LC-MS/MS, and 24 of which were precisely visualized by using MALDI-MSI. The lipids, including phosphatidylcholines (PC), phosphatidylethanolamines (PE) and triacylglycerols (TG) showed heterogeneous distribution patterns within the cotyledonary and radicle tissues. Additionally, the roles these lipids played in the metabolic pathways were analyzed, and relationship of the spatial distribution of LPC (lysophosphatidylcholine) and corresponding PC was studied. The unique distribution patterns of these lipid metabolites revealed by MSI can provide new insights into areas relating to the spatial compartmentation of lipid metabolism in plants. We believe that the results of MSI, if combined with transcriptomics and proteomics, may offer significant help in genetic engineering work.
KW - Cottonseed
KW - LC-MS/MS
KW - Lipids
KW - MALDI-MSI
KW - Spatial distribution
UR - http://www.scopus.com/inward/record.url?scp=85090335841&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2020.121614
DO - 10.1016/j.talanta.2020.121614
M3 - Journal article
C2 - 33076144
AN - SCOPUS:85090335841
SN - 0039-9140
VL - 221
JO - Talanta
JF - Talanta
M1 - 121614
ER -