@inbook{b475918ae516465c803d8a270109a4c7,
title = "Validation of the Interaction Between Stress-Responsive LncRNAs and RNA-Binding Proteins In Vitro",
abstract = "Plant long non-coding RNAs (lncRNAs) have been implicated in many biological processes, including responses to abiotic stresses, yet their detailed functions and especially their modes of action are still underexplored. lncRNAs often interact with proteins to participate in multiple levels of gene regulation. Therefore, identifying the RNA-binding proteins and validating their interaction with lncRNAs will be instrumental in revealing the functions of lncRNAs. Here, we describe two major methods to determine the interaction between lncRNA and proteins in vitro, RNA pull-down, and RNA EMSA.",
keywords = "LncRNA, RNA EMSA, RNA pull-down, RNA-binding protein",
author = "Liaoliao Ye and Tao Qin and Liming Xiong",
note = "Publisher Copyright: {\textcopyright} 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2024",
month = jun,
day = "13",
doi = "10.1007/978-1-0716-3973-3_9",
language = "English",
isbn = "9781071639726",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "133--144",
editor = "Ramanjulu Sunkar",
booktitle = "Plant Stress Tolerance",
edition = "3rd",
}