Using two-phase solvent systems for sample pretreatment increases yield of counter-current chromatography: Anemarrhena asphodeloides saponins, a case study

Meng Shun Liu, Siu Leung Chau, Edmond Dik Lung MA, Chung Hang Leung, Zhaoxiang BIAN, Aiping LYU, Hong Xi Xu*, Simon Q B HAN

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

This paper reports a way to increase the production yield of counter-current chromatography by using two-phase solvent systems in sample pretreatment. This strategy succeeds in the case of anti-EV71 saponin preparation from Anemarrhena asphodeloides. Briefly, 15 g of water extract was refined by solvent partition with ethyl acetate/1-butanol/water (1:4:5, v/v) to yield 4.17 g of the upper part, which in turns yielded 635 mg of timosaponin B-II (purity 96.5%) and 672 mg of anemarsaponin B (97.8%) after gradient CCC separation with 1-butanol/water (containing 0.01 mol/L CuSO4 in water, 1:1, v/v) and ethyl acetate/1-butanol/water (3:17:20, v/v/v). In a similar way, the ethanol extract (8 g) was partitioned between the diphase solvent system of ethyl acetate/1-butanol/water (4:1:5) to get 2.16 g of refined fraction and yielded 358 mg of timosaponin A-III (96.4%) by CCC elution using ethyl acetate-methanol-water (4:1:5, v/v/v). To remove mangiferin, the salt CuSO4 was added; it formed a complex with mangiferin, thereby enlarging the separation coefficient between mangiferin and timosaponin B-II.

Original languageEnglish
Pages (from-to)138-143
Number of pages6
JournalSeparation and Purification Technology
Volume138
DOIs
Publication statusPublished - 10 Dec 2014

Scopus Subject Areas

  • Analytical Chemistry
  • Filtration and Separation

User-Defined Keywords

  • Anemarrhena asphodeloides
  • Anemarsaponin B
  • Counter-current chromatography
  • Timosaponin A-III
  • Timosaponin B-II

Fingerprint

Dive into the research topics of 'Using two-phase solvent systems for sample pretreatment increases yield of counter-current chromatography: Anemarrhena asphodeloides saponins, a case study'. Together they form a unique fingerprint.

Cite this