TY - JOUR
T1 - Using two-phase solvent systems for sample pretreatment increases yield of counter-current chromatography
T2 - Anemarrhena asphodeloides saponins, a case study
AU - Liu, Meng Shun
AU - Chau, Siu Leung
AU - MA, Edmond Dik Lung
AU - Leung, Chung Hang
AU - BIAN, Zhaoxiang
AU - LYU, Aiping
AU - Xu, Hong Xi
AU - HAN, Simon Q B
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (Grant No. 81303289 ) and the Hong Kong Baptist University ( FRG2/12-13/006, FRG2/13-14/026 , and RC-start up grant).
PY - 2014/12/10
Y1 - 2014/12/10
N2 - This paper reports a way to increase the production yield of counter-current chromatography by using two-phase solvent systems in sample pretreatment. This strategy succeeds in the case of anti-EV71 saponin preparation from Anemarrhena asphodeloides. Briefly, 15 g of water extract was refined by solvent partition with ethyl acetate/1-butanol/water (1:4:5, v/v) to yield 4.17 g of the upper part, which in turns yielded 635 mg of timosaponin B-II (purity 96.5%) and 672 mg of anemarsaponin B (97.8%) after gradient CCC separation with 1-butanol/water (containing 0.01 mol/L CuSO4 in water, 1:1, v/v) and ethyl acetate/1-butanol/water (3:17:20, v/v/v). In a similar way, the ethanol extract (8 g) was partitioned between the diphase solvent system of ethyl acetate/1-butanol/water (4:1:5) to get 2.16 g of refined fraction and yielded 358 mg of timosaponin A-III (96.4%) by CCC elution using ethyl acetate-methanol-water (4:1:5, v/v/v). To remove mangiferin, the salt CuSO4 was added; it formed a complex with mangiferin, thereby enlarging the separation coefficient between mangiferin and timosaponin B-II.
AB - This paper reports a way to increase the production yield of counter-current chromatography by using two-phase solvent systems in sample pretreatment. This strategy succeeds in the case of anti-EV71 saponin preparation from Anemarrhena asphodeloides. Briefly, 15 g of water extract was refined by solvent partition with ethyl acetate/1-butanol/water (1:4:5, v/v) to yield 4.17 g of the upper part, which in turns yielded 635 mg of timosaponin B-II (purity 96.5%) and 672 mg of anemarsaponin B (97.8%) after gradient CCC separation with 1-butanol/water (containing 0.01 mol/L CuSO4 in water, 1:1, v/v) and ethyl acetate/1-butanol/water (3:17:20, v/v/v). In a similar way, the ethanol extract (8 g) was partitioned between the diphase solvent system of ethyl acetate/1-butanol/water (4:1:5) to get 2.16 g of refined fraction and yielded 358 mg of timosaponin A-III (96.4%) by CCC elution using ethyl acetate-methanol-water (4:1:5, v/v/v). To remove mangiferin, the salt CuSO4 was added; it formed a complex with mangiferin, thereby enlarging the separation coefficient between mangiferin and timosaponin B-II.
KW - Anemarrhena asphodeloides
KW - Anemarsaponin B
KW - Counter-current chromatography
KW - Timosaponin A-III
KW - Timosaponin B-II
UR - http://www.scopus.com/inward/record.url?scp=84910078749&partnerID=8YFLogxK
U2 - 10.1016/j.seppur.2014.10.011
DO - 10.1016/j.seppur.2014.10.011
M3 - Article
AN - SCOPUS:84910078749
VL - 138
SP - 138
EP - 143
JO - Separation and Purification Technology
JF - Separation and Purification Technology
SN - 1383-5866
ER -