Use of trichosanthin to reduce infection by turnip mosaic virus

Ying Hoo Lam, Yum Shing Wong, Bin Wang, Ricky N S WONG, Hin Wing Yeung, Pang Chui Shaw*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

Exogenous application of recombinant trichosanthin (TCS) inhibited local lesion formation by turnip mosaic virus (TuMV) in leaves of Nicotiana tabacum and caused a delay in the development of mosaic symptoms by TuMV in Brassica parachinensis. A dose-response relationship was observed in the latter case. To produce transgenic plants expressing a TCS cDNA, a chimeric gene which contains a fusion between the cauliflower mosaic virus 35S promoter, the TCS-encoding cDNA and the octopine synthase 3′ polyadenylation sequence (ocs 3′) was constructed. The chimeric gene was then cloned into a binary vector and introduced into the genome of N. tabacum cv. 'Wisconsin 38' via Agrobacterium tumefaciens-mediated transformation. Three transgenic tobacco plants which synthesized TCS protein in the cytosol were completely resistant to mechanical inoculation of TuMV.

Original languageEnglish
Pages (from-to)111-117
Number of pages7
JournalPlant Science
Volume114
Issue number1
DOIs
Publication statusPublished - 1996

Scopus Subject Areas

  • Genetics
  • Agronomy and Crop Science
  • Plant Science

User-Defined Keywords

  • Anti-viral assays
  • Brassica parachinensis
  • Nicotiana tabacum
  • Ribosome inactivating protein
  • Trichosanthin
  • Turnip mosaic virus

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