Use of trichosanthin to reduce infection by turnip mosaic virus

Ying Hoo Lam; Yum Shing Wong; Bin Wang; Ricky N S Wong; Hin Wing Yeung; Pang Chui Shaw

doi:10.1016/0168-9452(95)04310-1

Use of trichosanthin to reduce infection by turnip mosaic virus

Ying Hoo Lam, Yum Shing Wong, Bin Wang, Ricky N S Wong, Hin Wing Yeung, Pang Chui Shaw^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

66 Citations (Scopus)

Abstract

Exogenous application of recombinant trichosanthin (TCS) inhibited local lesion formation by turnip mosaic virus (TuMV) in leaves of Nicotiana tabacum and caused a delay in the development of mosaic symptoms by TuMV in Brassica parachinensis. A dose-response relationship was observed in the latter case. To produce transgenic plants expressing a TCS cDNA, a chimeric gene which contains a fusion between the cauliflower mosaic virus 35S promoter, the TCS-encoding cDNA and the octopine synthase 3′ polyadenylation sequence (ocs 3′) was constructed. The chimeric gene was then cloned into a binary vector and introduced into the genome of N. tabacum cv. 'Wisconsin 38' via Agrobacterium tumefaciens-mediated transformation. Three transgenic tobacco plants which synthesized TCS protein in the cytosol were completely resistant to mechanical inoculation of TuMV.

Original language	English
Pages (from-to)	111-117
Number of pages	7
Journal	Plant Science
Volume	114
Issue number	1
DOIs	https://doi.org/10.1016/0168-9452(95)04310-1
Publication status	Published - 9 Feb 1996

Scopus Subject Areas

Genetics
Agronomy and Crop Science
Plant Science

User-Defined Keywords

Anti-viral assays
Brassica parachinensis
Nicotiana tabacum
Ribosome inactivating protein
Trichosanthin
Turnip mosaic virus

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10.1016/0168-9452(95)04310-1

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title = "Use of trichosanthin to reduce infection by turnip mosaic virus",

abstract = "Exogenous application of recombinant trichosanthin (TCS) inhibited local lesion formation by turnip mosaic virus (TuMV) in leaves of Nicotiana tabacum and caused a delay in the development of mosaic symptoms by TuMV in Brassica parachinensis. A dose-response relationship was observed in the latter case. To produce transgenic plants expressing a TCS cDNA, a chimeric gene which contains a fusion between the cauliflower mosaic virus 35S promoter, the TCS-encoding cDNA and the octopine synthase 3′ polyadenylation sequence (ocs 3′) was constructed. The chimeric gene was then cloned into a binary vector and introduced into the genome of N. tabacum cv. 'Wisconsin 38' via Agrobacterium tumefaciens-mediated transformation. Three transgenic tobacco plants which synthesized TCS protein in the cytosol were completely resistant to mechanical inoculation of TuMV.",

keywords = "Anti-viral assays, Brassica parachinensis, Nicotiana tabacum, Ribosome inactivating protein, Trichosanthin, Turnip mosaic virus",

author = "Lam, {Ying Hoo} and Wong, {Yum Shing} and Bin Wang and Wong, {Ricky N S} and Yeung, {Hin Wing} and Shaw, {Pang Chui}",

note = "Funding Information: Thanksa red uet o Ms. M.H. Yung for thes eeds of theN . tubucum, Dr. J.D.G. Jones for A. tumefu-ciens LBA4404, pSLJ 4D4 and pSLJ 44024a nd Ms. WendyK o for advicei n the anti-virala ssays, This work was supportedi n part by a research grant (CUHK 11/91)f rom the ResearchG rants Council of Hong Kong.",

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doi = "10.1016/0168-9452(95)04310-1",

language = "English",

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AU - Lam, Ying Hoo

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AU - Wong, Ricky N S

AU - Yeung, Hin Wing

AU - Shaw, Pang Chui

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PY - 1996/2/9

Y1 - 1996/2/9

N2 - Exogenous application of recombinant trichosanthin (TCS) inhibited local lesion formation by turnip mosaic virus (TuMV) in leaves of Nicotiana tabacum and caused a delay in the development of mosaic symptoms by TuMV in Brassica parachinensis. A dose-response relationship was observed in the latter case. To produce transgenic plants expressing a TCS cDNA, a chimeric gene which contains a fusion between the cauliflower mosaic virus 35S promoter, the TCS-encoding cDNA and the octopine synthase 3′ polyadenylation sequence (ocs 3′) was constructed. The chimeric gene was then cloned into a binary vector and introduced into the genome of N. tabacum cv. 'Wisconsin 38' via Agrobacterium tumefaciens-mediated transformation. Three transgenic tobacco plants which synthesized TCS protein in the cytosol were completely resistant to mechanical inoculation of TuMV.

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Use of trichosanthin to reduce infection by turnip mosaic virus

Abstract

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