Use of LC fingerprinting to evaluate the quality of Erxian decoction

Yong Mei Hu; Stephen Cho Wing Sze; Shou Xun Zhao; Yao Tong

doi:10.1365/s10337-009-1218-5

Use of LC fingerprinting to evaluate the quality of Erxian decoction

Yong Mei Hu, Stephen Cho Wing Sze, Shou Xun Zhao, Yao Tong^*

^*Corresponding author for this work

Golden Meditech Centre for NeuroRegeneration Sciences

Research output: Contribution to journal › Journal article › peer-review

6 Citations (Scopus)

Abstract

High-performance liquid chromatography with diode-array detection has been used to evaluate the quality of Erxian decoction (EXD), a Chinese medicine prescription, by use of a combination of fingerprinting and simultaneous quantification of six marker compounds. The standard chromatographic fingerprint containing forty common peaks was constructed from seven batches of the EXD samples to evaluate batch-to-batch consistency. In addition, six representative bioactive markers were simultaneously quantified with good linearity, precision, repeatability, and accuracy. The fingerprint accompanied by quantification of the marker compounds can be used to evaluate the quality of EXD.

Original language	English
Pages (from-to)	613-618
Number of pages	6
Journal	Chromatographia
Volume	70
Issue number	3-4
DOIs	https://doi.org/10.1365/s10337-009-1218-5
Publication status	Published - Aug 2009

User-Defined Keywords

Column liquid chromatography
Diode-array detection
Erxian decoction
Traditional Chinese medicine
Quality evaluation
Fingerprint
Consistency

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10.1365/s10337-009-1218-5

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title = "Use of LC fingerprinting to evaluate the quality of Erxian decoction",

abstract = "High-performance liquid chromatography with diode-array detection has been used to evaluate the quality of Erxian decoction (EXD), a Chinese medicine prescription, by use of a combination of fingerprinting and simultaneous quantification of six marker compounds. The standard chromatographic fingerprint containing forty common peaks was constructed from seven batches of the EXD samples to evaluate batch-to-batch consistency. In addition, six representative bioactive markers were simultaneously quantified with good linearity, precision, repeatability, and accuracy. The fingerprint accompanied by quantification of the marker compounds can be used to evaluate the quality of EXD.",

keywords = "Column liquid chromatography, Diode-array detection, Erxian decoction, Traditional Chinese medicine, Quality evaluation, Fingerprint, Consistency",

author = "Hu, {Yong Mei} and Sze, {Stephen Cho Wing} and Zhao, {Shou Xun} and Yao Tong",

note = "Funding information: The research was supported by the Small Project Funding (200807176144) from the Committee on Research and Conference Grants, Li Ka Shing Faculty of Medicine, the University of Hong Kong. The authors would also like to thank the Physical & Chemical Testing Center at the Jinan University, Guangzhou, for their assistance in NMR and ESIMS experiments and the Aomei' Company in Hong Kong for supplying the EXD samples. Publisher copyright: {\textcopyright} 2009, Vieweg Teubner | GWV Fachverlage GmbH ",

year = "2009",

month = aug,

doi = "10.1365/s10337-009-1218-5",

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AU - Hu, Yong Mei

AU - Sze, Stephen Cho Wing

AU - Zhao, Shou Xun

AU - Tong, Yao

N1 - Funding information: The research was supported by the Small Project Funding (200807176144) from the Committee on Research and Conference Grants, Li Ka Shing Faculty of Medicine, the University of Hong Kong. The authors would also like to thank the Physical & Chemical Testing Center at the Jinan University, Guangzhou, for their assistance in NMR and ESIMS experiments and the Aomei' Company in Hong Kong for supplying the EXD samples. Publisher copyright: © 2009, Vieweg Teubner | GWV Fachverlage GmbH

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N2 - High-performance liquid chromatography with diode-array detection has been used to evaluate the quality of Erxian decoction (EXD), a Chinese medicine prescription, by use of a combination of fingerprinting and simultaneous quantification of six marker compounds. The standard chromatographic fingerprint containing forty common peaks was constructed from seven batches of the EXD samples to evaluate batch-to-batch consistency. In addition, six representative bioactive markers were simultaneously quantified with good linearity, precision, repeatability, and accuracy. The fingerprint accompanied by quantification of the marker compounds can be used to evaluate the quality of EXD.

AB - High-performance liquid chromatography with diode-array detection has been used to evaluate the quality of Erxian decoction (EXD), a Chinese medicine prescription, by use of a combination of fingerprinting and simultaneous quantification of six marker compounds. The standard chromatographic fingerprint containing forty common peaks was constructed from seven batches of the EXD samples to evaluate batch-to-batch consistency. In addition, six representative bioactive markers were simultaneously quantified with good linearity, precision, repeatability, and accuracy. The fingerprint accompanied by quantification of the marker compounds can be used to evaluate the quality of EXD.

KW - Column liquid chromatography

KW - Diode-array detection

KW - Erxian decoction

KW - Traditional Chinese medicine

KW - Quality evaluation

KW - Fingerprint

KW - Consistency

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DO - 10.1365/s10337-009-1218-5

M3 - Journal article

SN - 0009-5893

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SP - 613

EP - 618

JO - Chromatographia

JF - Chromatographia

IS - 3-4

ER -

Use of LC fingerprinting to evaluate the quality of Erxian decoction

Abstract

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