Use of capillary electrophoresis in the study of interaction between dsDNA and drug molecules

Juan Fang Wu*, Ling Xin Chen, Guo An Luo, Yong Suo Liu, Yi Ming Wang, Zhi Hong JIANG

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Two 17-mer dsDNA with different sequence characteristics were designed to investigate the binding characteristics of berberine, an anticancer drug with uncertain binding mode, and Hoechst 33258, a model DNA minor groove binder, with dsDNA, respectively by the capillary zone electrophoresis (CZE). Kenndler model analysis revealed that Hoechst 33258 exhibited intermediate affinity with dsDNA, while there was only low affinity and some weak binding preference for AATT-containing to GGCC-containing dsDNA for berberine.

Original languageEnglish
Pages (from-to)383-386
Number of pages4
JournalChinese Chemical Letters
Volume17
Issue number3
Publication statusPublished - Mar 2006

Scopus Subject Areas

  • Chemistry(all)

User-Defined Keywords

  • Berberine
  • Capillary zone electrophoresis
  • DNA
  • Hoechst 33258
  • Interaction

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