Use of capillary electrophoresis in the study of interaction between dsDNA and drug molecules

Juan Fang Wu; Ling Xin Chen; Guo An Luo; Yong Suo Liu; Yi Ming Wang; Zhi Hong Jiang

Use of capillary electrophoresis in the study of interaction between dsDNA and drug molecules

Juan Fang Wu, Ling Xin Chen, Guo An Luo^*, Yong Suo Liu, Yi Ming Wang, Zhi Hong Jiang

^*Corresponding author for this work

School of Chinese Medicine

Research output: Contribution to journal › Journal article › peer-review

3 Citations (Scopus)

Abstract

Two 17-mer dsDNA with different sequence characteristics were designed to investigate the binding characteristics of berberine, an anticancer drug with uncertain binding mode, and Hoechst 33258, a model DNA minor groove binder, with dsDNA, respectively by the capillary zone electrophoresis (CZE). Kenndler model analysis revealed that Hoechst 33258 exhibited intermediate affinity with dsDNA, while there was only low affinity and some weak binding preference for AATT-containing to GGCC-containing dsDNA for berberine.

Original language	English
Pages (from-to)	383-386
Number of pages	4
Journal	Chinese Chemical Letters
Volume	17
Issue number	3
Publication status	Published - Mar 2006

Scopus Subject Areas

Chemistry(all)

User-Defined Keywords

Berberine
Capillary zone electrophoresis
DNA
Hoechst 33258
Interaction

Cite this

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title = "Use of capillary electrophoresis in the study of interaction between dsDNA and drug molecules",

abstract = "Two 17-mer dsDNA with different sequence characteristics were designed to investigate the binding characteristics of berberine, an anticancer drug with uncertain binding mode, and Hoechst 33258, a model DNA minor groove binder, with dsDNA, respectively by the capillary zone electrophoresis (CZE). Kenndler model analysis revealed that Hoechst 33258 exhibited intermediate affinity with dsDNA, while there was only low affinity and some weak binding preference for AATT-containing to GGCC-containing dsDNA for berberine.",

keywords = "Berberine, Capillary zone electrophoresis, DNA, Hoechst 33258, Interaction",

author = "Wu, {Juan Fang} and Chen, {Ling Xin} and Luo, {Guo An} and Liu, {Yong Suo} and Wang, {Yi Ming} and Jiang, {Zhi Hong}",

year = "2006",

month = mar,

language = "English",

volume = "17",

pages = "383--386",

journal = "Chinese Chemical Letters",

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T1 - Use of capillary electrophoresis in the study of interaction between dsDNA and drug molecules

AU - Wu, Juan Fang

AU - Chen, Ling Xin

AU - Luo, Guo An

AU - Liu, Yong Suo

AU - Wang, Yi Ming

AU - Jiang, Zhi Hong

PY - 2006/3

Y1 - 2006/3

N2 - Two 17-mer dsDNA with different sequence characteristics were designed to investigate the binding characteristics of berberine, an anticancer drug with uncertain binding mode, and Hoechst 33258, a model DNA minor groove binder, with dsDNA, respectively by the capillary zone electrophoresis (CZE). Kenndler model analysis revealed that Hoechst 33258 exhibited intermediate affinity with dsDNA, while there was only low affinity and some weak binding preference for AATT-containing to GGCC-containing dsDNA for berberine.

AB - Two 17-mer dsDNA with different sequence characteristics were designed to investigate the binding characteristics of berberine, an anticancer drug with uncertain binding mode, and Hoechst 33258, a model DNA minor groove binder, with dsDNA, respectively by the capillary zone electrophoresis (CZE). Kenndler model analysis revealed that Hoechst 33258 exhibited intermediate affinity with dsDNA, while there was only low affinity and some weak binding preference for AATT-containing to GGCC-containing dsDNA for berberine.

KW - Berberine

KW - Capillary zone electrophoresis

KW - DNA

KW - Hoechst 33258

KW - Interaction

UR - https://library.hkbu.edu.hk/record/?ID=alma991026766922603409&T=PC

UR - http://www.scopus.com/inward/record.url?scp=33645365501&partnerID=8YFLogxK

M3 - Journal article

AN - SCOPUS:33645365501

SN - 1001-8417

VL - 17

SP - 383

EP - 386

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 3

ER -

Use of capillary electrophoresis in the study of interaction between dsDNA and drug molecules

Abstract

Scopus Subject Areas

User-Defined Keywords

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