Urinary bromophenol glucuronide and sulfate conjugates: Potential human exposure molecular markers for polybrominated diphenyl ethers

Ka Lok Ho; Man Shan Yau; Margaret B. Murphy; Yi Wan; Bonnie M.W. Fong; Sidney Tam; John P. Giesy; Kelvin S Y LEUNG; Michael H.W. Lam

doi:10.1016/j.chemosphere.2015.03.003

Urinary bromophenol glucuronide and sulfate conjugates: Potential human exposure molecular markers for polybrominated diphenyl ethers

Ka Lok Ho
, Man Shan Yau
, Margaret B. Murphy^*
, Yi Wan
, Bonnie M.W. Fong
, Sidney Tam
, John P. Giesy
, Kelvin S Y LEUNG
, Michael H.W. Lam

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

26 Citations (Scopus)

Abstract

One possible source of urinary bromophenol (BP) glucuronide and sulfate conjugates in mammalian animal models and humans is polybromodiphenyl ethers (PBDEs), a group of additive flame-retardants found ubiquitously in the environment. In order to study the correlation between levels of PBDEs in human blood plasma and those of the corresponding BP-conjugates in human urine, concentrations of 17 BDE congeners, 22 OH-BDE and 13 MeO-BDE metabolites, and 3 BPs in plasma collected from 100 voluntary donors in Hong Kong were measured by gas chromatograph tandem mass spectrometry (GC-MS). Geometric mean concentration of σPBDEs, σOH-BDEs, σMeO-BDEs and σBPs in human plasma were 4.45ngg^-1lw, 1.88ngg^-1lw, 0.42ngg^-1lw and 1.59ngg^-1lw respectively. Concentrations of glucuronide and sulfate conjugates of 2,4-dibromophenol (2,4-DBP) and 2,4,6-tribromophenol (2,4,6-TBP) in paired samples of urine were determined by liquid chromatography tandem triple quadrupole mass spectrometry (LC-MS/MS). BP-conjugates were found in all of the parallel urine samples, in the range of 0.08-106.49μgg^-1-creatinine. Correlations among plasma concentrations of σPBDEs/σOH-BDEs/σMeO-BDEs/σBPs and BP-conjugates in urine were evaluated by multivariate regression and Pearson product correlation analyses. These urinary BP-conjugates were positively correlated with σPBDEs in blood plasma, but were either not or negatively correlated with other organobromine compounds in blood plasma. Stronger correlations (Pearson's r as great as 0.881) were observed between concentrations of BDE congeners having the same number and pattern of bromine substitution on their phenyl rings in blood plasma and their corresponding BP-conjugates in urine.

Original language	English
Pages (from-to)	6-12
Number of pages	7
Journal	Chemosphere
Volume	133
DOIs	https://doi.org/10.1016/j.chemosphere.2015.03.003
Publication status	Published - 1 Aug 2015

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

Bromophenols
Exposure molecular markers
Human blood plasma
Human urine
Metabolites
Polybrominated diphenyl ethers

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10.1016/j.chemosphere.2015.03.003

Cite this

@article{b38856eecc4b42a49947575967063d1a,

title = "Urinary bromophenol glucuronide and sulfate conjugates: Potential human exposure molecular markers for polybrominated diphenyl ethers",

abstract = "One possible source of urinary bromophenol (BP) glucuronide and sulfate conjugates in mammalian animal models and humans is polybromodiphenyl ethers (PBDEs), a group of additive flame-retardants found ubiquitously in the environment. In order to study the correlation between levels of PBDEs in human blood plasma and those of the corresponding BP-conjugates in human urine, concentrations of 17 BDE congeners, 22 OH-BDE and 13 MeO-BDE metabolites, and 3 BPs in plasma collected from 100 voluntary donors in Hong Kong were measured by gas chromatograph tandem mass spectrometry (GC-MS). Geometric mean concentration of σPBDEs, σOH-BDEs, σMeO-BDEs and σBPs in human plasma were 4.45ngg-1lw, 1.88ngg-1lw, 0.42ngg-1lw and 1.59ngg-1lw respectively. Concentrations of glucuronide and sulfate conjugates of 2,4-dibromophenol (2,4-DBP) and 2,4,6-tribromophenol (2,4,6-TBP) in paired samples of urine were determined by liquid chromatography tandem triple quadrupole mass spectrometry (LC-MS/MS). BP-conjugates were found in all of the parallel urine samples, in the range of 0.08-106.49μgg-1-creatinine. Correlations among plasma concentrations of σPBDEs/σOH-BDEs/σMeO-BDEs/σBPs and BP-conjugates in urine were evaluated by multivariate regression and Pearson product correlation analyses. These urinary BP-conjugates were positively correlated with σPBDEs in blood plasma, but were either not or negatively correlated with other organobromine compounds in blood plasma. Stronger correlations (Pearson's r as great as 0.881) were observed between concentrations of BDE congeners having the same number and pattern of bromine substitution on their phenyl rings in blood plasma and their corresponding BP-conjugates in urine.",

keywords = "Bromophenols, Exposure molecular markers, Human blood plasma, Human urine, Metabolites, Polybrominated diphenyl ethers",

author = "Ho, \{Ka Lok\} and Yau, \{Man Shan\} and Murphy, \{Margaret B.\} and Yi Wan and Fong, \{Bonnie M.W.\} and Sidney Tam and Giesy, \{John P.\} and LEUNG, \{Kelvin S Y\} and Lam, \{Michael H.W.\}",

note = "Funding Information: This work is support by a grant from Research Grants Council of the Hong Kong Special Administrative Region, China [Reference No. CityU 9041623]. Prof. Giesy was supported by the program of 2012 “Great Concentration Foreign Experts” (\#GDW20123200120) funded by the State Administration of Foreign Experts Affairs, the P.R. China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences. He was also supported by the Canada Research Chair program, a Visiting Distinguished Professorship in the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong. ",

year = "2015",

month = aug,

day = "1",

doi = "10.1016/j.chemosphere.2015.03.003",

language = "English",

volume = "133",

pages = "6--12",

journal = "Chemosphere",

issn = "0045-6535",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Urinary bromophenol glucuronide and sulfate conjugates

T2 - Potential human exposure molecular markers for polybrominated diphenyl ethers

AU - Ho, Ka Lok

AU - Yau, Man Shan

AU - Murphy, Margaret B.

AU - Wan, Yi

AU - Fong, Bonnie M.W.

AU - Tam, Sidney

AU - Giesy, John P.

AU - LEUNG, Kelvin S Y

AU - Lam, Michael H.W.

N1 - Funding Information: This work is support by a grant from Research Grants Council of the Hong Kong Special Administrative Region, China [Reference No. CityU 9041623]. Prof. Giesy was supported by the program of 2012 “Great Concentration Foreign Experts” (#GDW20123200120) funded by the State Administration of Foreign Experts Affairs, the P.R. China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences. He was also supported by the Canada Research Chair program, a Visiting Distinguished Professorship in the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong.

PY - 2015/8/1

Y1 - 2015/8/1

N2 - One possible source of urinary bromophenol (BP) glucuronide and sulfate conjugates in mammalian animal models and humans is polybromodiphenyl ethers (PBDEs), a group of additive flame-retardants found ubiquitously in the environment. In order to study the correlation between levels of PBDEs in human blood plasma and those of the corresponding BP-conjugates in human urine, concentrations of 17 BDE congeners, 22 OH-BDE and 13 MeO-BDE metabolites, and 3 BPs in plasma collected from 100 voluntary donors in Hong Kong were measured by gas chromatograph tandem mass spectrometry (GC-MS). Geometric mean concentration of σPBDEs, σOH-BDEs, σMeO-BDEs and σBPs in human plasma were 4.45ngg-1lw, 1.88ngg-1lw, 0.42ngg-1lw and 1.59ngg-1lw respectively. Concentrations of glucuronide and sulfate conjugates of 2,4-dibromophenol (2,4-DBP) and 2,4,6-tribromophenol (2,4,6-TBP) in paired samples of urine were determined by liquid chromatography tandem triple quadrupole mass spectrometry (LC-MS/MS). BP-conjugates were found in all of the parallel urine samples, in the range of 0.08-106.49μgg-1-creatinine. Correlations among plasma concentrations of σPBDEs/σOH-BDEs/σMeO-BDEs/σBPs and BP-conjugates in urine were evaluated by multivariate regression and Pearson product correlation analyses. These urinary BP-conjugates were positively correlated with σPBDEs in blood plasma, but were either not or negatively correlated with other organobromine compounds in blood plasma. Stronger correlations (Pearson's r as great as 0.881) were observed between concentrations of BDE congeners having the same number and pattern of bromine substitution on their phenyl rings in blood plasma and their corresponding BP-conjugates in urine.

AB - One possible source of urinary bromophenol (BP) glucuronide and sulfate conjugates in mammalian animal models and humans is polybromodiphenyl ethers (PBDEs), a group of additive flame-retardants found ubiquitously in the environment. In order to study the correlation between levels of PBDEs in human blood plasma and those of the corresponding BP-conjugates in human urine, concentrations of 17 BDE congeners, 22 OH-BDE and 13 MeO-BDE metabolites, and 3 BPs in plasma collected from 100 voluntary donors in Hong Kong were measured by gas chromatograph tandem mass spectrometry (GC-MS). Geometric mean concentration of σPBDEs, σOH-BDEs, σMeO-BDEs and σBPs in human plasma were 4.45ngg-1lw, 1.88ngg-1lw, 0.42ngg-1lw and 1.59ngg-1lw respectively. Concentrations of glucuronide and sulfate conjugates of 2,4-dibromophenol (2,4-DBP) and 2,4,6-tribromophenol (2,4,6-TBP) in paired samples of urine were determined by liquid chromatography tandem triple quadrupole mass spectrometry (LC-MS/MS). BP-conjugates were found in all of the parallel urine samples, in the range of 0.08-106.49μgg-1-creatinine. Correlations among plasma concentrations of σPBDEs/σOH-BDEs/σMeO-BDEs/σBPs and BP-conjugates in urine were evaluated by multivariate regression and Pearson product correlation analyses. These urinary BP-conjugates were positively correlated with σPBDEs in blood plasma, but were either not or negatively correlated with other organobromine compounds in blood plasma. Stronger correlations (Pearson's r as great as 0.881) were observed between concentrations of BDE congeners having the same number and pattern of bromine substitution on their phenyl rings in blood plasma and their corresponding BP-conjugates in urine.

KW - Bromophenols

KW - Exposure molecular markers

KW - Human blood plasma

KW - Human urine

KW - Metabolites

KW - Polybrominated diphenyl ethers

UR - https://www.scopus.com/pages/publications/84928888782

U2 - 10.1016/j.chemosphere.2015.03.003

DO - 10.1016/j.chemosphere.2015.03.003

M3 - Journal article

C2 - 25817024

AN - SCOPUS:84928888782

SN - 0045-6535

VL - 133

SP - 6

EP - 12

JO - Chemosphere

JF - Chemosphere

ER -

Urinary bromophenol glucuronide and sulfate conjugates: Potential human exposure molecular markers for polybrominated diphenyl ethers

Abstract

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