TY - JOUR
T1 - Urinary bromophenol glucuronide and sulfate conjugates
T2 - Potential human exposure molecular markers for polybrominated diphenyl ethers
AU - Ho, Ka Lok
AU - Yau, Man Shan
AU - Murphy, Margaret B.
AU - Wan, Yi
AU - Fong, Bonnie M.W.
AU - Tam, Sidney
AU - Giesy, John P.
AU - LEUNG, Kelvin S Y
AU - Lam, Michael H.W.
N1 - Funding Information:
This work is support by a grant from Research Grants Council of the Hong Kong Special Administrative Region, China [Reference No. CityU 9041623]. Prof. Giesy was supported by the program of 2012 “Great Concentration Foreign Experts” (#GDW20123200120) funded by the State Administration of Foreign Experts Affairs, the P.R. China to Nanjing University and the Einstein Professor Program of the Chinese Academy of Sciences. He was also supported by the Canada Research Chair program, a Visiting Distinguished Professorship in the Department of Biology and Chemistry and State Key Laboratory in Marine Pollution, City University of Hong Kong.
PY - 2015/8/1
Y1 - 2015/8/1
N2 - One possible source of urinary bromophenol (BP) glucuronide and sulfate conjugates in mammalian animal models and humans is polybromodiphenyl ethers (PBDEs), a group of additive flame-retardants found ubiquitously in the environment. In order to study the correlation between levels of PBDEs in human blood plasma and those of the corresponding BP-conjugates in human urine, concentrations of 17 BDE congeners, 22 OH-BDE and 13 MeO-BDE metabolites, and 3 BPs in plasma collected from 100 voluntary donors in Hong Kong were measured by gas chromatograph tandem mass spectrometry (GC-MS). Geometric mean concentration of σPBDEs, σOH-BDEs, σMeO-BDEs and σBPs in human plasma were 4.45ngg-1lw, 1.88ngg-1lw, 0.42ngg-1lw and 1.59ngg-1lw respectively. Concentrations of glucuronide and sulfate conjugates of 2,4-dibromophenol (2,4-DBP) and 2,4,6-tribromophenol (2,4,6-TBP) in paired samples of urine were determined by liquid chromatography tandem triple quadrupole mass spectrometry (LC-MS/MS). BP-conjugates were found in all of the parallel urine samples, in the range of 0.08-106.49μgg-1-creatinine. Correlations among plasma concentrations of σPBDEs/σOH-BDEs/σMeO-BDEs/σBPs and BP-conjugates in urine were evaluated by multivariate regression and Pearson product correlation analyses. These urinary BP-conjugates were positively correlated with σPBDEs in blood plasma, but were either not or negatively correlated with other organobromine compounds in blood plasma. Stronger correlations (Pearson's r as great as 0.881) were observed between concentrations of BDE congeners having the same number and pattern of bromine substitution on their phenyl rings in blood plasma and their corresponding BP-conjugates in urine.
AB - One possible source of urinary bromophenol (BP) glucuronide and sulfate conjugates in mammalian animal models and humans is polybromodiphenyl ethers (PBDEs), a group of additive flame-retardants found ubiquitously in the environment. In order to study the correlation between levels of PBDEs in human blood plasma and those of the corresponding BP-conjugates in human urine, concentrations of 17 BDE congeners, 22 OH-BDE and 13 MeO-BDE metabolites, and 3 BPs in plasma collected from 100 voluntary donors in Hong Kong were measured by gas chromatograph tandem mass spectrometry (GC-MS). Geometric mean concentration of σPBDEs, σOH-BDEs, σMeO-BDEs and σBPs in human plasma were 4.45ngg-1lw, 1.88ngg-1lw, 0.42ngg-1lw and 1.59ngg-1lw respectively. Concentrations of glucuronide and sulfate conjugates of 2,4-dibromophenol (2,4-DBP) and 2,4,6-tribromophenol (2,4,6-TBP) in paired samples of urine were determined by liquid chromatography tandem triple quadrupole mass spectrometry (LC-MS/MS). BP-conjugates were found in all of the parallel urine samples, in the range of 0.08-106.49μgg-1-creatinine. Correlations among plasma concentrations of σPBDEs/σOH-BDEs/σMeO-BDEs/σBPs and BP-conjugates in urine were evaluated by multivariate regression and Pearson product correlation analyses. These urinary BP-conjugates were positively correlated with σPBDEs in blood plasma, but were either not or negatively correlated with other organobromine compounds in blood plasma. Stronger correlations (Pearson's r as great as 0.881) were observed between concentrations of BDE congeners having the same number and pattern of bromine substitution on their phenyl rings in blood plasma and their corresponding BP-conjugates in urine.
KW - Bromophenols
KW - Exposure molecular markers
KW - Human blood plasma
KW - Human urine
KW - Metabolites
KW - Polybrominated diphenyl ethers
UR - http://www.scopus.com/inward/record.url?scp=84928888782&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2015.03.003
DO - 10.1016/j.chemosphere.2015.03.003
M3 - Journal article
C2 - 25817024
AN - SCOPUS:84928888782
SN - 0045-6535
VL - 133
SP - 6
EP - 12
JO - Chemosphere
JF - Chemosphere
ER -
