Unravelling calcium-alleviated aluminium toxicity in Arabidopsis thaliana: Insights into regulatory mechanisms using proteomics

Juan Chen*, Rui Xue Duan, Wen Jun Hu, Ni Na Zhang, Xue Yuan Lin, Jianhua ZHANG, Hai Lei Zheng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

Aluminium (Al) toxicity is a major limiting factor for plant productivity in acidic soils. Calcium (Ca) is an essential element and participates in various physiological responses to environmental stress. Here, the aim of this work was to study the role of exogenous Ca in alleviating Al toxicity in Arabidopsis thaliana. For that we used the methods of physiology and proteomics. Results showed that Ca alleviated Al-induced growth inhibition and decreased Al accumulation. Proteomic analyses showed that 75 differentially expressed protein spots, including those related to organic acid metabolism, cell wall components, cellular transport, signal transduction and antioxidant activity, transcription and protein metabolism were identified during the response of Arabidopsis to Ca alleviated Al toxicity. Ca regulated tricarboxylic acid (TCA) cycle-related protein abundances and affected organic acid concentrations and related enzyme activities under Al stress. Vacuolar and mitochondrion adenosine triphosphate (ATP) synthase, and cell wall component-related proteins played important roles in Ca-alleviated Al toxicity. Ethylene-insensitive 3 (EIN3) participated in Ca-alleviated Al toxicity. Glutathione S-transferase (GST6) and glutathione S-transferase tau 19 (ATGSTU19) were associated with antioxidant activities induced by Ca under Al stress. Our results may contribute to an understanding of the functional mechanism by which Ca alleviates Al stress in plants. Significant: Our results elucidated how Ca alleviate the effects of Al toxicity on the inhibition of plant growth and Al accumulation in plants using the proteomics and physiological methods, which may contribute to a better understanding of the molecular mechanism of Ca alleviation Al stress in plants.

Original languageEnglish
Pages (from-to)15-30
Number of pages16
JournalJournal of Proteomics
Volume199
DOIs
Publication statusPublished - 15 May 2019

Scopus Subject Areas

  • Biophysics
  • Biochemistry

User-Defined Keywords

  • Aluminium toxicity
  • Calcium
  • Organic acid
  • Proteomics
  • Signal transduction
  • TCA cycle

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