Underwater cytometer for in situ measurement of marine phytoplankton by a technique combining laser-induced fluorescence and laser Doppler velocimetry

Xuzhu Wang*, Robert K Y CHAN, Amelia S.K. Cheng

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

3 Citations (Scopus)

Abstract

We present a new type of flow cytometer that can operate underwater for a long time, as long as days, for measuring the size distribution, concentration, and biomass of marine phytoplankton. The major improvement of the instrument over existing techniques is the elimination of sample preparation, which is achieved with a laser Doppler crossed-beam arrangement for both defining a measurement volume and measuring the speed of the particle traversing it. By simultaneously sampling the laser-induced fluorescence signal and the Doppler signals, the technique can discriminate sizes of phytoplankton.

Original languageEnglish
Pages (from-to)1087-1089
Number of pages3
JournalOptics Letters
Volume30
Issue number10
DOIs
Publication statusPublished - 15 May 2005

Scopus Subject Areas

  • Atomic and Molecular Physics, and Optics

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