TY - JOUR
T1 - Ultrasound-assisted extraction of triterpenoids from Chaenomeles speciosa leaves
T2 - Process optimization, adsorptive enrichment, chemical profiling, and protection against ulcerative colitis
AU - Hou, Mengyang
AU - Shi, Jingchun
AU - Lin, Chengyuan
AU - Zhu, Lin
AU - Bian, Zhaoxiang
N1 - Funding Information:
This work was funded by the Health@InnoHK Initiative Fund of the Hong Kong Special Administrative Region Government (ITC RC/IHK/4/7) and the Key-Area Research and Development Program of Guangdong Province (2020B1111110003).
Publisher Copyright:
© 2024 The Author(s). Published by Elsevier B.V.
PY - 2024/12
Y1 - 2024/12
N2 - For the valorization of Chaenomeles speciosa leaves, this study focused on extraction, enrichment, chemical profiling, and investigation of the biological activity of its abundant triterpenoid components. Initially, the total triterpenoids in C. speciosa leaves were extracted by ultrasonic-assisted extraction (UAE) method, with the extraction process optimized through response surface methodology (RSM). Under the optimal conditions of extraction solvent 93 % EtOH, ultrasound power 390 W, extraction time 30 min, extraction temperature 70 °C, liquid-to-solid ratio 25 mL/g, and 2 extraction cycles, the maximum total triterpenoids yield (TTY) reached 36.77 ± 0.40 mg/g. The total triterpenoids in the crude extract were subsequently enriched by X-5 resin column chromatography, resulting in a fourfold increase in purity, reaching 73.27 ± 0.84 %. Thirteen compounds in the triterpenoid-rich fraction (TRF) were identified through UPLC–QTOF-MS/MS, and five major triterpenoids (oleanolic acid, ursolic acid, betulinic acid, maslinic acid, and pomolic acid) were simultaneously quantified by HPLC–QQQ-MS. Furthermore, TRF demonstrated a notable amelioration against dextran sodium sulfate (DSS)-induced ulcerative colitis in mice, indicating its promise as a potent intervention for this condition. In summary, this study will contribute to enhancing the utilization efficiency of Chaenomeles speciosa leaves.
AB - For the valorization of Chaenomeles speciosa leaves, this study focused on extraction, enrichment, chemical profiling, and investigation of the biological activity of its abundant triterpenoid components. Initially, the total triterpenoids in C. speciosa leaves were extracted by ultrasonic-assisted extraction (UAE) method, with the extraction process optimized through response surface methodology (RSM). Under the optimal conditions of extraction solvent 93 % EtOH, ultrasound power 390 W, extraction time 30 min, extraction temperature 70 °C, liquid-to-solid ratio 25 mL/g, and 2 extraction cycles, the maximum total triterpenoids yield (TTY) reached 36.77 ± 0.40 mg/g. The total triterpenoids in the crude extract were subsequently enriched by X-5 resin column chromatography, resulting in a fourfold increase in purity, reaching 73.27 ± 0.84 %. Thirteen compounds in the triterpenoid-rich fraction (TRF) were identified through UPLC–QTOF-MS/MS, and five major triterpenoids (oleanolic acid, ursolic acid, betulinic acid, maslinic acid, and pomolic acid) were simultaneously quantified by HPLC–QQQ-MS. Furthermore, TRF demonstrated a notable amelioration against dextran sodium sulfate (DSS)-induced ulcerative colitis in mice, indicating its promise as a potent intervention for this condition. In summary, this study will contribute to enhancing the utilization efficiency of Chaenomeles speciosa leaves.
KW - Chaenomeles speciosa leaves
KW - Enrichment
KW - Triterpenoids
KW - Ultrasound-assisted extraction
KW - UPLC-QTOF-MS/MS, HPLC-QQQ-MS, Ulcerative colitis
UR - http://www.scopus.com/inward/record.url?scp=85207782290&partnerID=8YFLogxK
U2 - 10.1016/j.ultsonch.2024.107136
DO - 10.1016/j.ultsonch.2024.107136
M3 - Journal article
C2 - 39492129
AN - SCOPUS:85207782290
SN - 1350-4177
VL - 111
JO - Ultrasonics Sonochemistry
JF - Ultrasonics Sonochemistry
M1 - 107136
ER -