TY - JOUR
T1 - Two proteins with ribosome-inactivating, cytotoxic and abortifacient activities from seeds of Luffa cylindrica roem (Cucurbitaceae)
AU - Ng, T. B.
AU - WONG, Ricky N S
AU - Yeung, H. W.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1992
Y1 - 1992
N2 - Two immunologically distinct glycoproteins, with a molecular weight of 28,000 and 28,5000 respectively, were isolated from seeds of Luffa cylindrica using a procedure that involved acetone precipitation, gel filtration on Sephadex G-75 and ion exchange chromatography on CM-Sepharose CL-6B. The proteins differed in the content of aspartic acid, threonine, proline and alanine but were otherwise similar in amino acid composition. The proteins were capable of inducing mid-term abortion in mice, inhibiting protein synthesis in a cell-free system and suppressing thymidine uptake by human choriocarcinoma cells.
AB - Two immunologically distinct glycoproteins, with a molecular weight of 28,000 and 28,5000 respectively, were isolated from seeds of Luffa cylindrica using a procedure that involved acetone precipitation, gel filtration on Sephadex G-75 and ion exchange chromatography on CM-Sepharose CL-6B. The proteins differed in the content of aspartic acid, threonine, proline and alanine but were otherwise similar in amino acid composition. The proteins were capable of inducing mid-term abortion in mice, inhibiting protein synthesis in a cell-free system and suppressing thymidine uptake by human choriocarcinoma cells.
UR - https://pubmed.ncbi.nlm.nih.gov/1503559/
UR - http://www.scopus.com/inward/record.url?scp=0026648952&partnerID=8YFLogxK
M3 - Journal article
C2 - 1503559
AN - SCOPUS:0026648952
SN - 0158-5231
VL - 27
SP - 197
EP - 207
JO - Biochemistry International
JF - Biochemistry International
IS - 2
ER -