Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

Filip Kielar; Aileen Congreve; Ga Lai Law; Elizabeth J. New; David Parker; Ka Leung Wong; Pilar Castreňo; Javier De Mendoza

doi:10.1039/b803864c

Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

Filip Kielar, Aileen Congreve, Ga Lai Law, Elizabeth J. New, David Parker^*, Ka Leung Wong, Pilar Castreňo, Javier De Mendoza

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

89 Citations (Scopus)

Abstract

An emissive terbium complex has been conjugated to a C₁₂ chain, Lys-Arg₇, Arg₇, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC₅₀12 μM), the C₁₂-amide complex giving rise to necrotic cell death in skin fibroblasts (IC₅₀ 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.

Original language	English
Pages (from-to)	2435-2437
Number of pages	3
Journal	Chemical Communications
Issue number	21
DOIs	https://doi.org/10.1039/b803864c
Publication status	Published - 22 Apr 2008

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title = "Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates",

abstract = "An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.",

author = "Filip Kielar and Aileen Congreve and Law, {Ga Lai} and New, {Elizabeth J.} and David Parker and Wong, {Ka Leung} and Pilar Castre{\v n}o and {De Mendoza}, Javier",

note = "We thank EPSRC, the Royal Society (D. P.), the EC networks of excellence (EMIL and DIMI) and the Association of Commonwealth Universities for a scholarship (E. J. N.). Publisher Copyright: {\textcopyright} The Royal Society of Chemistry 2008",

year = "2008",

month = apr,

day = "22",

doi = "10.1039/b803864c",

language = "English",

pages = "2435--2437",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

AU - Kielar, Filip

AU - Congreve, Aileen

AU - Law, Ga Lai

AU - New, Elizabeth J.

AU - Parker, David

AU - Wong, Ka Leung

AU - Castreňo, Pilar

AU - De Mendoza, Javier

N1 - We thank EPSRC, the Royal Society (D. P.), the EC networks of excellence (EMIL and DIMI) and the Association of Commonwealth Universities for a scholarship (E. J. N.). Publisher Copyright: © The Royal Society of Chemistry 2008

PY - 2008/4/22

Y1 - 2008/4/22

N2 - An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.

AB - An emissive terbium complex has been conjugated to a C12 chain, Lys-Arg7, Arg7, a tetraguanidinium cation and human serum albumin; two-photon excitation at 720 nm facilitated microscopy studies revealing cell localisation profiles with the oligo-guanidinium conjugate localising in mitochondria but causing apoptotic cell death (IC5012 μM), the C12-amide complex giving rise to necrotic cell death in skin fibroblasts (IC50 8 μM) and the peptide conjugates and the methyl ester generating punctuate cytosolic intracellular distributions.

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UR - https://pubs.rsc.org/en/content/articlelanding/2008/cc/b803864c

U2 - 10.1039/b803864c

DO - 10.1039/b803864c

M3 - Journal article

C2 - 18491006

AN - SCOPUS:44049103229

SN - 1359-7345

SP - 2435

EP - 2437

JO - Chemical Communications

JF - Chemical Communications

IS - 21

ER -

Two-photon microscopy study of the intracellular compartmentalisation of emissive terbium complexes and their oligo-arginine and oligo-guanidinium conjugates

Abstract

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