Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex-Silver Nanoparticle Platform

Jinshui Liu; Zhen Zhen Dong; Chao Yang; Guodong Li; Chun Wu; Fu Wa Lee; Chung Hang Leung; Dik Lung Ma

doi:10.1038/s41598-017-09478-6

Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex-Silver Nanoparticle Platform

Jinshui Liu, Zhen Zhen Dong, Chao Yang, Guodong Li, Chun Wu, Fu Wa Lee, Chung Hang Leung, Dik Lung Ma^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

29 Citations (Scopus)

Abstract

A sensitive turn-on luminescent sensor for H₂O₂ based on the silver nanoparticle (AgNP)-mediated quenching of an luminescent Ir(III) complex (Ir-1) has been designed. In the absence of H₂O₂, the luminescence intensity of Ir-1 can be quenched by AgNPs via non-radiative energy transfer. However, H₂O₂ can oxidize AgNPs to soluble Ag⁺ cations, which restores the luminescence of Ir-1. The sensing platform displayed a sensitive response to H₂O₂ in the range of 0-17 μM, with a detection limit of 0.3 μM. Importantly, the probe was successfully applied to monitor intracellular H₂O₂ in living cells, and it also showed high selectivity for H₂O₂ over other interfering substances.

Original language	English
Article number	8980
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-09478-6
Publication status	Published - 1 Dec 2017

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title = "Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex-Silver Nanoparticle Platform",

abstract = "A sensitive turn-on luminescent sensor for H2O2 based on the silver nanoparticle (AgNP)-mediated quenching of an luminescent Ir(III) complex (Ir-1) has been designed. In the absence of H2O2, the luminescence intensity of Ir-1 can be quenched by AgNPs via non-radiative energy transfer. However, H2O2 can oxidize AgNPs to soluble Ag+ cations, which restores the luminescence of Ir-1. The sensing platform displayed a sensitive response to H2O2 in the range of 0-17 μM, with a detection limit of 0.3 μM. Importantly, the probe was successfully applied to monitor intracellular H2O2 in living cells, and it also showed high selectivity for H2O2 over other interfering substances.",

author = "Jinshui Liu and Dong, {Zhen Zhen} and Chao Yang and Guodong Li and Chun Wu and Lee, {Fu Wa} and Leung, {Chung Hang} and Ma, {Dik Lung}",

note = "Funding Information: This work is supported by Hong Kong Baptist University (FRG2/15–16/002), the Health and Medical Research Fund (HMRF/14130522, 14150561), the Research Grants Council (HKBU/12301115, HKBU/204612 and HKBU/201913), the National Natural Science Foundation of China (21575121, 21628502), the Guangdong Province Natural Science Foundation (2015A030313816), the Hong Kong Baptist University Century Club Sponsorship Scheme 2016, the Interdisciplinary Research Matching Scheme (RC-IRMS/15–16/03), Innovation and Technology Fund (ITS/260/16FX), Matching Proof of Concept Fund (MPCF-001-2017/18), the Science and Technology Development Fund, Macao SAR (077/2016/A2, 007/2014/AMJ), and the University of Macau (MYRG2015–00137-ICMS-QRCM, MYRG2016–00151-ICMS-QRCM and MRG044/LCH/2015/ICMS).",

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AU - Yang, Chao

AU - Li, Guodong

AU - Wu, Chun

AU - Lee, Fu Wa

AU - Leung, Chung Hang

AU - Ma, Dik Lung

N1 - Funding Information: This work is supported by Hong Kong Baptist University (FRG2/15–16/002), the Health and Medical Research Fund (HMRF/14130522, 14150561), the Research Grants Council (HKBU/12301115, HKBU/204612 and HKBU/201913), the National Natural Science Foundation of China (21575121, 21628502), the Guangdong Province Natural Science Foundation (2015A030313816), the Hong Kong Baptist University Century Club Sponsorship Scheme 2016, the Interdisciplinary Research Matching Scheme (RC-IRMS/15–16/03), Innovation and Technology Fund (ITS/260/16FX), Matching Proof of Concept Fund (MPCF-001-2017/18), the Science and Technology Development Fund, Macao SAR (077/2016/A2, 007/2014/AMJ), and the University of Macau (MYRG2015–00137-ICMS-QRCM, MYRG2016–00151-ICMS-QRCM and MRG044/LCH/2015/ICMS).

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N2 - A sensitive turn-on luminescent sensor for H2O2 based on the silver nanoparticle (AgNP)-mediated quenching of an luminescent Ir(III) complex (Ir-1) has been designed. In the absence of H2O2, the luminescence intensity of Ir-1 can be quenched by AgNPs via non-radiative energy transfer. However, H2O2 can oxidize AgNPs to soluble Ag+ cations, which restores the luminescence of Ir-1. The sensing platform displayed a sensitive response to H2O2 in the range of 0-17 μM, with a detection limit of 0.3 μM. Importantly, the probe was successfully applied to monitor intracellular H2O2 in living cells, and it also showed high selectivity for H2O2 over other interfering substances.

AB - A sensitive turn-on luminescent sensor for H2O2 based on the silver nanoparticle (AgNP)-mediated quenching of an luminescent Ir(III) complex (Ir-1) has been designed. In the absence of H2O2, the luminescence intensity of Ir-1 can be quenched by AgNPs via non-radiative energy transfer. However, H2O2 can oxidize AgNPs to soluble Ag+ cations, which restores the luminescence of Ir-1. The sensing platform displayed a sensitive response to H2O2 in the range of 0-17 μM, with a detection limit of 0.3 μM. Importantly, the probe was successfully applied to monitor intracellular H2O2 in living cells, and it also showed high selectivity for H2O2 over other interfering substances.

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Turn-on Luminescent Probe for Hydrogen Peroxide Sensing and Imaging in Living Cells based on an Iridium(III) Complex-Silver Nanoparticle Platform

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