TY - JOUR
T1 - Trimester-specific urinary metabolome alterations associated with gestational diabetes mellitus
T2 - A study in different pregnancy stages
AU - Zhao, Hongzhi
AU - Zheng, Yuanyuan
AU - Zhu, Lin
AU - Xiang, Li
AU - Xu, Shunqing
AU - Cai, Zongwei
N1 - Funding Information:
The work was financially supported by National Natural Science Foundation of China (Nos. 42177412 and 21437002) and National Key Research and Development Program of China (Nos. 2017YFC1600500 and 2019YFC1804602)
Publisher Copyright:
© 2021
PY - 2022/6
Y1 - 2022/6
N2 - Gestational diabetes mellitus (GDM), a frequently-occurring disease during pregnancy, may cause some adverse healthy outcome of both mother and offspring. However, the knowledge about metabolite alterations during the pathogenesis and development process is limited. Here, a large longitudinal non-targeted metabolomics study of 195 pregnant women (64 women with subsequently developed GDM and 131 healthy controls) was conducted. Each participant provided urine samples at three timepoints during early, middle and late pregnancy, respectively. The metabolic profiles of 585 urine samples (195 × 3) were measured by using ultra-high performance liquid chromatography coupled with Orbitrap high-resolution mass spectrometry. Among the 56 identified metabolites, the levels of eight metabolites increased and three ones decreased in the first trimester, the concentration of one metabolite increased and those of 20 decreased in the second trimester, as well as the levels of five metabolites increased and two decreased in the third trimester. After false discovery rate correction, the levels of valine and 5-acetamidovalerate in GDM group significantly increased in the first trimester, the levels of 1-methylguanine and 1,3-dihydro-(2H)-indol-2-one significantly decreased in the second trimester and three metabolites (threonine, OH-octanedioyl-carnitine and pimelylcarnitine) increased and N-acetyltryptophan decreased in the third trimester, respectively. Six metabolites, such as pantothenic acid and threonine, had significant interaction effects between gestational stage (different trimester) and group (GDM or control). The differential metabolites were involved in “tryptophan metabolism”, “purine metabolism”, “valine, leucine and isoleucine degradation” and other pathways. The findings may provide insights into further pathogenesis study of GDM.
AB - Gestational diabetes mellitus (GDM), a frequently-occurring disease during pregnancy, may cause some adverse healthy outcome of both mother and offspring. However, the knowledge about metabolite alterations during the pathogenesis and development process is limited. Here, a large longitudinal non-targeted metabolomics study of 195 pregnant women (64 women with subsequently developed GDM and 131 healthy controls) was conducted. Each participant provided urine samples at three timepoints during early, middle and late pregnancy, respectively. The metabolic profiles of 585 urine samples (195 × 3) were measured by using ultra-high performance liquid chromatography coupled with Orbitrap high-resolution mass spectrometry. Among the 56 identified metabolites, the levels of eight metabolites increased and three ones decreased in the first trimester, the concentration of one metabolite increased and those of 20 decreased in the second trimester, as well as the levels of five metabolites increased and two decreased in the third trimester. After false discovery rate correction, the levels of valine and 5-acetamidovalerate in GDM group significantly increased in the first trimester, the levels of 1-methylguanine and 1,3-dihydro-(2H)-indol-2-one significantly decreased in the second trimester and three metabolites (threonine, OH-octanedioyl-carnitine and pimelylcarnitine) increased and N-acetyltryptophan decreased in the third trimester, respectively. Six metabolites, such as pantothenic acid and threonine, had significant interaction effects between gestational stage (different trimester) and group (GDM or control). The differential metabolites were involved in “tryptophan metabolism”, “purine metabolism”, “valine, leucine and isoleucine degradation” and other pathways. The findings may provide insights into further pathogenesis study of GDM.
KW - Dynamic metabolic profile
KW - Gestational diabetes mellitus
KW - High-resolution mass spectrometry
KW - Maternal urine
KW - Non-targeted metabolomics
UR - http://www.scopus.com/inward/record.url?scp=85127332871&partnerID=8YFLogxK
U2 - 10.1016/j.cclet.2021.10.001
DO - 10.1016/j.cclet.2021.10.001
M3 - Journal article
AN - SCOPUS:85127332871
SN - 1001-8417
VL - 33
SP - 3139
EP - 3143
JO - Chinese Chemical Letters
JF - Chinese Chemical Letters
IS - 6
ER -