Trichosanthin can spontaneously penetrate phospholipid monolayer under acid condition

Xiaofeng Xia; Shaoxiong Wang; Jianbang Luo; Ricky N S WONG; Senfang Sui

doi:10.1007/BF02886348

Trichosanthin can spontaneously penetrate phospholipid monolayer under acid condition

Xiaofeng Xia, Shaoxiong Wang, Jianbang Luo, Ricky N S WONG, Senfang Sui^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

8 Citations (Scopus)

Abstract

Tianhuafen is a kind of traditional Chinese medicine for abortion, which has long been used in China. The basic protein trichosanthin (TCS) is responsible for such activity. As a ribosome inactivating protein (RIP), trichosanthin removes A₄₃₀₄ in the 28s rRNA via the N-glycosidase activity and inactivates the ribosome. However, it remains unclear how TCS can enter the cytoplasm. In the experiment, monolayers at air/water interface have been used to study the interaction between TCS and phospholipid membrane. The results show that TCS can penetrate negatively charged phospholipid monolayer under acid condition, and its membrane penetrating ability is obviously pH-dependent. A hypothesis of TCS penetrating biological membrane under acid condition is proposed based on the obtained result.

Original language	English
Pages (from-to)	1892-1895
Number of pages	4
Journal	Chinese Science Bulletin
Volume	44
Issue number	20
DOIs	https://doi.org/10.1007/BF02886348
Publication status	Published - Oct 1999

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User-Defined Keywords

Critical insertion pressure
Lipid monolayer
Trichosanthin (TCS)

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10.1007/BF02886348

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@article{54e2a9daca794d6b83312521524517d3,

title = "Trichosanthin can spontaneously penetrate phospholipid monolayer under acid condition",

abstract = "Tianhuafen is a kind of traditional Chinese medicine for abortion, which has long been used in China. The basic protein trichosanthin (TCS) is responsible for such activity. As a ribosome inactivating protein (RIP), trichosanthin removes A4304 in the 28s rRNA via the N-glycosidase activity and inactivates the ribosome. However, it remains unclear how TCS can enter the cytoplasm. In the experiment, monolayers at air/water interface have been used to study the interaction between TCS and phospholipid membrane. The results show that TCS can penetrate negatively charged phospholipid monolayer under acid condition, and its membrane penetrating ability is obviously pH-dependent. A hypothesis of TCS penetrating biological membrane under acid condition is proposed based on the obtained result.",

keywords = "Critical insertion pressure, Lipid monolayer, Trichosanthin (TCS)",

author = "Xiaofeng Xia and Shaoxiong Wang and Jianbang Luo and WONG, {Ricky N S} and Senfang Sui",

note = "Funding Information: Acknowledgement This work was supported by the Coalition Foundation of Tsinghua University-HongKong Baptist University-Beijing University of Chinese Medicine and the National Natural Science Foundation of China (Grant Yo. 69731010).",

year = "1999",

month = oct,

doi = "10.1007/BF02886348",

language = "English",

volume = "44",

pages = "1892--1895",

journal = "Science Bulletin",

issn = "2095-9273",

publisher = "Elsevier BV",

number = "20",

}

TY - JOUR

T1 - Trichosanthin can spontaneously penetrate phospholipid monolayer under acid condition

AU - Xia, Xiaofeng

AU - Wang, Shaoxiong

AU - Luo, Jianbang

AU - WONG, Ricky N S

AU - Sui, Senfang

N1 - Funding Information: Acknowledgement This work was supported by the Coalition Foundation of Tsinghua University-HongKong Baptist University-Beijing University of Chinese Medicine and the National Natural Science Foundation of China (Grant Yo. 69731010).

PY - 1999/10

Y1 - 1999/10

N2 - Tianhuafen is a kind of traditional Chinese medicine for abortion, which has long been used in China. The basic protein trichosanthin (TCS) is responsible for such activity. As a ribosome inactivating protein (RIP), trichosanthin removes A4304 in the 28s rRNA via the N-glycosidase activity and inactivates the ribosome. However, it remains unclear how TCS can enter the cytoplasm. In the experiment, monolayers at air/water interface have been used to study the interaction between TCS and phospholipid membrane. The results show that TCS can penetrate negatively charged phospholipid monolayer under acid condition, and its membrane penetrating ability is obviously pH-dependent. A hypothesis of TCS penetrating biological membrane under acid condition is proposed based on the obtained result.

AB - Tianhuafen is a kind of traditional Chinese medicine for abortion, which has long been used in China. The basic protein trichosanthin (TCS) is responsible for such activity. As a ribosome inactivating protein (RIP), trichosanthin removes A4304 in the 28s rRNA via the N-glycosidase activity and inactivates the ribosome. However, it remains unclear how TCS can enter the cytoplasm. In the experiment, monolayers at air/water interface have been used to study the interaction between TCS and phospholipid membrane. The results show that TCS can penetrate negatively charged phospholipid monolayer under acid condition, and its membrane penetrating ability is obviously pH-dependent. A hypothesis of TCS penetrating biological membrane under acid condition is proposed based on the obtained result.

KW - Critical insertion pressure

KW - Lipid monolayer

KW - Trichosanthin (TCS)

UR - http://www.scopus.com/inward/record.url?scp=0001207270&partnerID=8YFLogxK

U2 - 10.1007/BF02886348

DO - 10.1007/BF02886348

M3 - Journal article

AN - SCOPUS:0001207270

SN - 2095-9273

VL - 44

SP - 1892

EP - 1895

JO - Science Bulletin

JF - Science Bulletin

IS - 20

ER -

Trichosanthin can spontaneously penetrate phospholipid monolayer under acid condition

Abstract

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