Translocation of Fungicides and Their Efficacy in Controlling Phellinus noxius, the Cause of Brown Root Rot Disease

Ting Zhi Liao, Yu Hsuan Chen, Jyh Nong Tsai, Chieh Chao, Tzu Pi Huang, Cheng Fang Hong, Zong Chi Wu, Isheng Jason Tsai, Hsin Han Lee, Ned B. Klopfenstein, Mee Sook Kim, Jane E. Stewart, Ndeme Atibalentja, Fred E. Brooks, Philip G. Cannon, A. Mohd Farid, Tsutomu Hattori, Hoi Shan Kwan, Regent Yau Ching Lam, Yuko OtaNorio Sahashi, Robert L. Schlub, Louise S. Shuey, Alvin M. C. Tang, Chia Lin Chung*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

Abstract

Brown root rot disease (BRRD), caused by Phellinus noxius, is an important tree disease in tropical and subtropical areas. To improve chemical control of BRRD and deter emergence of fungicide resistance in P. noxius, this study investigated control efficacies and systemic activities of fungicides with different modes of action. Fourteen fungicides with 11 different modes of action were tested for inhibitory effects in vitro on 39 P. noxius isolates from Taiwan, Hong Kong, Malaysia, Australia, and Pacific Islands. Cyproconazole, epoxiconazole, and tebuconazole (Fungicide Resistance Action Committee [FRAC] 3, target-site G1) inhibited colony growth of P. noxius by 99.9 to 100% at 10 ppm and 97.7 to 99.8% at 1 ppm. The other effective fungicide was cyprodinil + fludioxonil (FRAC 9 + 12, target-site D1 + E2), which showed growth inhibition of 96.9% at 10 ppm and 88.6% at 1 ppm. Acropetal translocation of six selected fungicides was evaluated in bishop wood (Bischofia javanica) seedlings by immersion of the root tips in each fungicide at 100 ppm, followed by liquid or gas chromatography tandem mass spectrometry analyses of consecutive segments of root, stem, and leaf tissues at 7 and 21 days posttreatment. Bidirectional translocation of the fungicides was also evaluated by stem injection of fungicide stock solutions. Cyproconazole and tebuconazole were the most readily absorbed by roots and efficiently transported acropetally. Greenhouse experiments suggested that cyproconazole, tebuconazole, and epoxiconazole have a slightly higher potential for controlling BRRD than mepronil, prochloraz, and cyprodinil + fludioxonil. Because all tested fungicides lacked basipetal translocation, soil drenching should be considered instead of trunk injection for their use in BRRD control.

Original languageEnglish
Pages (from-to)2039-2053
Number of pages15
JournalPlant Disease
Volume107
Issue number7
Early online date29 Jun 2023
DOIs
Publication statusPublished - Jul 2023

Scopus Subject Areas

  • Agronomy and Crop Science
  • Plant Science

User-Defined Keywords

  • Bischofia javanica
  • bishop wood
  • gas chromatography tandem mass spectrometry (GC-MS/MS)
  • liquid chromatography tandem mass spectrometry (LC-MS/MS)
  • Phellinus noxius
  • systemic fungicide

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