Transcriptomic analysis of photosynthesis-related genes regulated by alternate wetting and drying irrigation in flag leaves of rice

Tao Song; Feng Yang; Debatosh Das; Moxian Chen; Qijuan Hu; Yuan Tian; Chaolin Cheng; Yue Liu; Jianhua ZHANG

doi:10.1002/fes3.221

Transcriptomic analysis of photosynthesis-related genes regulated by alternate wetting and drying irrigation in flag leaves of rice

Tao Song, Feng Yang, Debatosh Das, Moxian Chen, Qijuan Hu, Yuan Tian, Chaolin Cheng, Yue Liu, Jianhua ZHANG^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

14 Citations (Scopus)

Abstract

Alternate wetting and drying (AWD) irrigation has been widely used to replace continuous flooding (CF), which does not result in yield loss while improving water productivity. However, it is still unexplored as to how this agricultural practice would affect gene expression to control plant physiology. In this study, we found that photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), chlorophyll (Chl) content, and leaf water potential (Lwp) significantly decreased while abscisic acid (ABA) content and water use efficiency (WUE) increased when soil water potential reached to −15 kPa in the AWD irrigation regime. We then analyzed the differences between AWD and CF irrigation practices in transcriptomic profiles for flag leaves. Genes were found to be differentially expressed in photosynthesis related pathways namely, Chl biosynthesis, photosynthesis-antenna proteins pathway, photosynthesis process, and ABA signal transduction pathway. The study provides important information on the morphological adaptations observed in the flag leaves in response to AWD irrigation, which will act as a basic framework to conduct molecular studies for improving rice cultivation.

Original language	English
Article number	e221
Journal	Food and Energy Security
Volume	9
Issue number	3
DOIs	https://doi.org/10.1002/fes3.221
Publication status	Published - 1 Aug 2020

Scopus Subject Areas

Forestry
Food Science
Renewable Energy, Sustainability and the Environment
Agronomy and Crop Science

User-Defined Keywords

abscisic acid
chlorophyll
light-harvesting complex
photosynthesis
transcriptome

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/fes3.221

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@article{8e7705a2178f4d32a382cd8aa077fbb6,

title = "Transcriptomic analysis of photosynthesis-related genes regulated by alternate wetting and drying irrigation in flag leaves of rice",

abstract = "Alternate wetting and drying (AWD) irrigation has been widely used to replace continuous flooding (CF), which does not result in yield loss while improving water productivity. However, it is still unexplored as to how this agricultural practice would affect gene expression to control plant physiology. In this study, we found that photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), chlorophyll (Chl) content, and leaf water potential (Lwp) significantly decreased while abscisic acid (ABA) content and water use efficiency (WUE) increased when soil water potential reached to −15 kPa in the AWD irrigation regime. We then analyzed the differences between AWD and CF irrigation practices in transcriptomic profiles for flag leaves. Genes were found to be differentially expressed in photosynthesis related pathways namely, Chl biosynthesis, photosynthesis-antenna proteins pathway, photosynthesis process, and ABA signal transduction pathway. The study provides important information on the morphological adaptations observed in the flag leaves in response to AWD irrigation, which will act as a basic framework to conduct molecular studies for improving rice cultivation.",

keywords = "abscisic acid, chlorophyll, light-harvesting complex, photosynthesis, transcriptome",

author = "Tao Song and Feng Yang and Debatosh Das and Moxian Chen and Qijuan Hu and Yuan Tian and Chaolin Cheng and Yue Liu and Jianhua ZHANG",

note = "Funding Information: This work was supported by the National Key Research and Development Program of China (2017YFE0118100 and 2018YFD02003025), the China Postdoctoral Science Foundation (2019M663122), the National Natural Science Foundation of China (31761130073 and 31872169), the Shenzhen Virtual University Park Support Scheme to CUHK Shenzhen Research Institute, the Natural Science Foundation of Hunan Province (2019JJ50263) and the Hong Kong Research Grant Council (AoE/M-05/12, AoE/M-403/16, CUHK14122415, 14160516, and 14177617). Funding Information: This work was supported by the National Key Research and Development Program of China (2017YFE0118100 and 2018YFD02003025), the China Postdoctoral Science Foundation (2019M663122), the National Natural Science Foundation of China (31761130073 and 31872169), the Shenzhen Virtual University Park Support Scheme to CUHK Shenzhen Research Institute, the Natural Science Foundation of Hunan Province (2019JJ50263) and the Hong Kong Research Grant Council (AoE/M‐05/12, AoE/M‐403/16, CUHK14122415, 14160516, and 14177617). ",

year = "2020",

month = aug,

day = "1",

doi = "10.1002/fes3.221",

language = "English",

volume = "9",

journal = "Food and Energy Security",

issn = "2048-3694",

publisher = "Wiley-Blackwell Publishing Ltd",

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T1 - Transcriptomic analysis of photosynthesis-related genes regulated by alternate wetting and drying irrigation in flag leaves of rice

AU - Song, Tao

AU - Yang, Feng

AU - Das, Debatosh

AU - Chen, Moxian

AU - Hu, Qijuan

AU - Tian, Yuan

AU - Cheng, Chaolin

AU - Liu, Yue

AU - ZHANG, Jianhua

N1 - Funding Information: This work was supported by the National Key Research and Development Program of China (2017YFE0118100 and 2018YFD02003025), the China Postdoctoral Science Foundation (2019M663122), the National Natural Science Foundation of China (31761130073 and 31872169), the Shenzhen Virtual University Park Support Scheme to CUHK Shenzhen Research Institute, the Natural Science Foundation of Hunan Province (2019JJ50263) and the Hong Kong Research Grant Council (AoE/M-05/12, AoE/M-403/16, CUHK14122415, 14160516, and 14177617). Funding Information: This work was supported by the National Key Research and Development Program of China (2017YFE0118100 and 2018YFD02003025), the China Postdoctoral Science Foundation (2019M663122), the National Natural Science Foundation of China (31761130073 and 31872169), the Shenzhen Virtual University Park Support Scheme to CUHK Shenzhen Research Institute, the Natural Science Foundation of Hunan Province (2019JJ50263) and the Hong Kong Research Grant Council (AoE/M‐05/12, AoE/M‐403/16, CUHK14122415, 14160516, and 14177617).

PY - 2020/8/1

Y1 - 2020/8/1

N2 - Alternate wetting and drying (AWD) irrigation has been widely used to replace continuous flooding (CF), which does not result in yield loss while improving water productivity. However, it is still unexplored as to how this agricultural practice would affect gene expression to control plant physiology. In this study, we found that photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), chlorophyll (Chl) content, and leaf water potential (Lwp) significantly decreased while abscisic acid (ABA) content and water use efficiency (WUE) increased when soil water potential reached to −15 kPa in the AWD irrigation regime. We then analyzed the differences between AWD and CF irrigation practices in transcriptomic profiles for flag leaves. Genes were found to be differentially expressed in photosynthesis related pathways namely, Chl biosynthesis, photosynthesis-antenna proteins pathway, photosynthesis process, and ABA signal transduction pathway. The study provides important information on the morphological adaptations observed in the flag leaves in response to AWD irrigation, which will act as a basic framework to conduct molecular studies for improving rice cultivation.

AB - Alternate wetting and drying (AWD) irrigation has been widely used to replace continuous flooding (CF), which does not result in yield loss while improving water productivity. However, it is still unexplored as to how this agricultural practice would affect gene expression to control plant physiology. In this study, we found that photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), chlorophyll (Chl) content, and leaf water potential (Lwp) significantly decreased while abscisic acid (ABA) content and water use efficiency (WUE) increased when soil water potential reached to −15 kPa in the AWD irrigation regime. We then analyzed the differences between AWD and CF irrigation practices in transcriptomic profiles for flag leaves. Genes were found to be differentially expressed in photosynthesis related pathways namely, Chl biosynthesis, photosynthesis-antenna proteins pathway, photosynthesis process, and ABA signal transduction pathway. The study provides important information on the morphological adaptations observed in the flag leaves in response to AWD irrigation, which will act as a basic framework to conduct molecular studies for improving rice cultivation.

KW - abscisic acid

KW - chlorophyll

KW - light-harvesting complex

KW - photosynthesis

KW - transcriptome

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U2 - 10.1002/fes3.221

DO - 10.1002/fes3.221

M3 - Journal article

AN - SCOPUS:85087215880

SN - 2048-3694

VL - 9

JO - Food and Energy Security

JF - Food and Energy Security

IS - 3

M1 - e221

ER -

Transcriptomic analysis of photosynthesis-related genes regulated by alternate wetting and drying irrigation in flag leaves of rice

Abstract

Scopus Subject Areas

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UN SDGs

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