Transcriptomic alterations in Daphnia magna embryos from mothers exposed to hypoxia

Keng Po Lai, Jing Woei Li, Christine Ying Shan Chan, Ting Fung Chan, Karen Wing Yee Yuen, Man Ying CHIU*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Hypoxia occurs when dissolved oxygen (DO) falls below 2.8 mg L−1 in aquatic environments. It can cause trans-generational effects not only in fish, but also in the water fleas Daphnia. In this study, transcriptome sequencing analysis was employed to identify transcriptomic alterations induced by hypoxia in embryos of Daphnia magna, with an aim to investigate the mechanism underlying the trans-generational effects caused by hypoxia in Daphnia. The embryos (F1) were collected from adults (F0) that were previously exposed to hypoxia (or normoxia) for their whole life. De novo transcriptome assembly identified 18270 transcripts that were matched to the UniProtKB/Swiss-Prot database and resulted in 7419 genes. Comparative transcriptome analysis showed 124 differentially expressed genes, including 70 up- and 54 down-regulated genes under hypoxia. Gene ontology analysis further highlighted three clusters of genes which revealed acclimatory changes of haemoglobin, suppression in vitellogenin gene family and histone modifications. Specifically, the expressions of histone H2B, H3, H4 and histone deacetylase 4 (HDAC4) were deregulated. This study suggested that trans-generational effects of hypoxia on Daphnia may be mediated through epigenetic regulations of histone modifications.

Original languageEnglish
Pages (from-to)454-463
Number of pages10
JournalAquatic Toxicology
Volume177
DOIs
Publication statusPublished - 1 Aug 2016

Scopus Subject Areas

  • Aquatic Science
  • Health, Toxicology and Mutagenesis

User-Defined Keywords

  • Embryonic development
  • Haemoglobin
  • Histone
  • Trans-generational effect
  • Vitellogenin

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