TY - JOUR
T1 - Tissue-specific metabolite profiling and quantitative analysis of ginsenosides in Panax quinquefolium using laser microdissection and liquid chromatography-quadrupole/time of flight-mass spectrometry
AU - Chen, Yujie
AU - Xu, Liang
AU - Zhao, Yuancen
AU - ZHAO, Zhongzhen
AU - CHEN, Hubiao
AU - YI, Tao
AU - Qin, Minjian
AU - LIANG, Zhitao
N1 - Publisher Copyright:
© 2015 Chen et al.
PY - 2015/12/9
Y1 - 2015/12/9
N2 - Background: The root of Panax quinquefolium L., famous as American ginseng all over the world, is one of the most widely-used medicinal or edible materials. Ginsenosides are recognized as the main bioactive chemical components responsible for various functions of American ginseng. In this study, tissue-specific chemicals of P. quinquefolium were analyzed by laser microdissection and ultra-high performance liquid chromatography- quadrupole/time-of-flight-mass spectrometry (UHPLC-Q/TOF-MS) to elucidate the distribution pattern of ginsenosides in tissues. The contents of ginsenosides in various tissues were also compared. Results: A total of 34 peaks were identified or temporarily identified in the chromatograms of tissue extractions. The cork, primary xylem or cortex contained higher contents of ginsenosides than phloem, secondary xylem and cambium. Thus, it would be reasonable to deduce that the ratio of total areas of cork, primary xylem and the cortex to the area of the whole transection could help to judge the quality of American ginseng by microscopic characteristics. Conclusion: This study sheds new light on the role of microscopic research in quality evaluation, and provides useful information for probing the biochemical pathways of ginsenosides. Graphical abstract.
AB - Background: The root of Panax quinquefolium L., famous as American ginseng all over the world, is one of the most widely-used medicinal or edible materials. Ginsenosides are recognized as the main bioactive chemical components responsible for various functions of American ginseng. In this study, tissue-specific chemicals of P. quinquefolium were analyzed by laser microdissection and ultra-high performance liquid chromatography- quadrupole/time-of-flight-mass spectrometry (UHPLC-Q/TOF-MS) to elucidate the distribution pattern of ginsenosides in tissues. The contents of ginsenosides in various tissues were also compared. Results: A total of 34 peaks were identified or temporarily identified in the chromatograms of tissue extractions. The cork, primary xylem or cortex contained higher contents of ginsenosides than phloem, secondary xylem and cambium. Thus, it would be reasonable to deduce that the ratio of total areas of cork, primary xylem and the cortex to the area of the whole transection could help to judge the quality of American ginseng by microscopic characteristics. Conclusion: This study sheds new light on the role of microscopic research in quality evaluation, and provides useful information for probing the biochemical pathways of ginsenosides. Graphical abstract.
KW - Ginsenosides
KW - Laser microdissection
KW - Panax quinquefolium L.
KW - Tissue-specific
KW - UHPLC-Q/TOF-MS
UR - http://www.scopus.com/inward/record.url?scp=84949663093&partnerID=8YFLogxK
U2 - 10.1186/s13065-015-0141-0
DO - 10.1186/s13065-015-0141-0
M3 - Journal article
AN - SCOPUS:84949663093
SN - 1752-153X
VL - 9
SP - 1
EP - 13
JO - Chemistry Central Journal
JF - Chemistry Central Journal
IS - 1
M1 - 66
ER -