Tissue-based metabolite profiling and qualitative comparison of two species of Achyranthes roots by use of UHPLC-QTOF MS and laser micro-dissection

Yogini Jaiswal, Zhitao Liang, Alan Ho, Hubiao Chen, Leonard Williams, Zhongzhen Zhao*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

17 Citations (Scopus)

Abstract

Achyranthes bidentata and Achyranthes aspera are saponin and steroid rich medicinal plants, used extensively for therapeutic treatments in Traditional Chinese Medicine (TCM) and Ayurveda. A. bidentata is reported to be one of the rare and extensively exploited medicinal plant species that face the issue of being endangered. Finding qualitative substitute with identical phyto-constituents contributing to similar composition and pharmacological benefits will help in reducing the burden of exploitation of the natural habitats of such plants. In the present study, a comparative metabolite analysis of the whole drug and specific tissues isolated by laser micro-dissection (LMD) was carried out for both the selected species, by use of ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-QTOF MS). The results of the study indicate that the cortex and the medullary ray tissues are rich in their content of steroidal and saponin constituents such as (25S)-inokosterone-20,22-acetonide, ginsenoside Ro, bidentatoside II and achyranthoside B. Metabolite profiling of the whole tissues of both the species indicates presence of identical constituents. Thus, it is inferred that A. bidentata and A. aspera can be used as qualitative substitutes for each other.

Original languageEnglish
Pages (from-to)10-19
Number of pages10
JournalJournal of Pharmaceutical Analysis
Volume8
Issue number1
Early online date16 Jun 2017
DOIs
Publication statusPublished - Feb 2018

Scopus Subject Areas

  • Analytical Chemistry
  • Pharmacy
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Electrochemistry

User-Defined Keywords

  • Achyranthes aspera
  • Achyranthes bidentata
  • Glycosides
  • Saponins
  • Steroids

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