Tian Xian Liquid (TXL) induces apoptosis in HT-29 colon cancer cell in vitro and inhibits tumor growth in vivo

Qing Liu, Yao Tong*, Stephen Cho Wing Sze, Wing Keung Liu, Lam Lam, Ellie Shihng Meir Chu, Christine Miu Ngan Yow

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background
Tian Xian Liquid (TXL) is a Chinese medicine decoction and has been used as an anticancer dietary supplement. The present study aims to investigate the effects of TXL on the apoptosis of HT-29 cells and tumor growth in vivo.

Method
HT-29 colon cancer cells were treated with gradient dilution of TXL. The mitochondrial membrane potential was measured by JC-1 assay. The release of cytochrome c from mitochondrial and apoptosis-related proteins Bax, Bcl-2, cleaved caspase-3, 9 were examined by Western blot analysis. HT-29 cells were implanted in nude mice to examine the effects of TXL on tumor growth.

Result
TXL inhibited HT-29 xenografted model and showed a strong and dose-dependent inhibitory effect on the proliferation of HT-29 cells. Mitochondrial membrane potential was reduced by TXL at the concentration of 0.5% above. For Western blot analysis, an increase in Bax expression and a decrease in Bcl-2 expression were observed in TXL-treated cells. TXL treatment increased the protein level of cleaved casepase-3 and caspase-9, and the release of cytochrome c in cytoplasm was up-regulated as well.

Conclusion
TXL significantly inhibits cell proliferation in the HT-29 cells and HT-29 xenografted model via the mitochondrial cell death pathway.
Original languageEnglish
Article number25
JournalChinese Medicine
Volume5
DOIs
Publication statusPublished - 21 Jul 2010
Externally publishedYes

Scopus Subject Areas

  • Medicine(all)

User-Defined Keywords

  • Mitochondrial Membrane Potential
  • Radix Astragalus
  • Radix Ginseng
  • Advanced Colon Cancer
  • Induce Cancer Cell Apoptosis

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