Three-way junction-promoted recycling amplification for sensitive DNA detection using highly bright DNA-silver nanocluster as label-free output

Fumiao Shen; He Qian; Yuliang Cheng; Yunfei Xie; Hang Yu; Weirong Yao; Renjun Pei; Y. Guo; Hung Wing Li

doi:10.1016/j.talanta.2019.120216

Three-way junction-promoted recycling amplification for sensitive DNA detection using highly bright DNA-silver nanocluster as label-free output

Fumiao Shen
, He Qian
, Yuliang Cheng
, Yunfei Xie
, Hang Yu
, Weirong Yao
, Renjun Pei
, Y. Guo^*
, Hung Wing Li^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

19 Citations (Scopus)

Abstract

In this work, an intriguing phenomenon was found and studied that the efficiency of exonuclease (Exo) III-assisted recycling amplification could be greatly promoted by the three-way junction structure. This was utilized as a visualizable detection strategy of DNA. Herein, highly bright DNA hairpin-templated silver nanoclusters (AgNCs) with quantum yield of 69.2% was firstly employed as the label-free signal output. The self-designed DNA hairpin was used as a molecular beacon by the relationship between the hairpin structure and the fluorescence signal of AgNCs. By recycling amplification of Exo-III, a significant change in fluorescence signal generated. Eventually, a label-free, visual, universal DNA detection platform based on Exo-III assisted amplification and DNA hairpin-AgNCs with high quantum yield was proposed.

Original language	English
Article number	120216
Journal	Talanta
Volume	206
DOIs	https://doi.org/10.1016/j.talanta.2019.120216
Publication status	Published - 1 Jan 2020

User-Defined Keywords

DNA detection
Label-free detection
Silver nanoclusters (AgNCs)
Three-way junction

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10.1016/j.talanta.2019.120216

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@article{182c822a4b7f4fefad42451ef85db87a,

title = "Three-way junction-promoted recycling amplification for sensitive DNA detection using highly bright DNA-silver nanocluster as label-free output",

abstract = "In this work, an intriguing phenomenon was found and studied that the efficiency of exonuclease (Exo) III-assisted recycling amplification could be greatly promoted by the three-way junction structure. This was utilized as a visualizable detection strategy of DNA. Herein, highly bright DNA hairpin-templated silver nanoclusters (AgNCs) with quantum yield of 69.2\% was firstly employed as the label-free signal output. The self-designed DNA hairpin was used as a molecular beacon by the relationship between the hairpin structure and the fluorescence signal of AgNCs. By recycling amplification of Exo-III, a significant change in fluorescence signal generated. Eventually, a label-free, visual, universal DNA detection platform based on Exo-III assisted amplification and DNA hairpin-AgNCs with high quantum yield was proposed.",

keywords = "DNA detection, Label-free detection, Silver nanoclusters (AgNCs), Three-way junction",

author = "Fumiao Shen and He Qian and Yuliang Cheng and Yunfei Xie and Hang Yu and Weirong Yao and Renjun Pei and Y. Guo and Li, \{Hung Wing\}",

note = "Funding Information: This work was supported by the National Key Research and Development Program of China (No. 2017YFC1601704 , 2018YFC1604202 , 2018YFC1602300 ), the National Science Foundation of China (No. 31601552 ), the National First-class Discipline Program of Food Science and Technology ( JUFSTR20180509 ) and the “HongKong Scholar” program. ",

year = "2020",

month = jan,

day = "1",

doi = "10.1016/j.talanta.2019.120216",

language = "English",

volume = "206",

journal = "Talanta",

issn = "0039-9140",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Three-way junction-promoted recycling amplification for sensitive DNA detection using highly bright DNA-silver nanocluster as label-free output

AU - Shen, Fumiao

AU - Qian, He

AU - Cheng, Yuliang

AU - Xie, Yunfei

AU - Yu, Hang

AU - Yao, Weirong

AU - Pei, Renjun

AU - Guo, Y.

AU - Li, Hung Wing

N1 - Funding Information: This work was supported by the National Key Research and Development Program of China (No. 2017YFC1601704 , 2018YFC1604202 , 2018YFC1602300 ), the National Science Foundation of China (No. 31601552 ), the National First-class Discipline Program of Food Science and Technology ( JUFSTR20180509 ) and the “HongKong Scholar” program.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - In this work, an intriguing phenomenon was found and studied that the efficiency of exonuclease (Exo) III-assisted recycling amplification could be greatly promoted by the three-way junction structure. This was utilized as a visualizable detection strategy of DNA. Herein, highly bright DNA hairpin-templated silver nanoclusters (AgNCs) with quantum yield of 69.2% was firstly employed as the label-free signal output. The self-designed DNA hairpin was used as a molecular beacon by the relationship between the hairpin structure and the fluorescence signal of AgNCs. By recycling amplification of Exo-III, a significant change in fluorescence signal generated. Eventually, a label-free, visual, universal DNA detection platform based on Exo-III assisted amplification and DNA hairpin-AgNCs with high quantum yield was proposed.

AB - In this work, an intriguing phenomenon was found and studied that the efficiency of exonuclease (Exo) III-assisted recycling amplification could be greatly promoted by the three-way junction structure. This was utilized as a visualizable detection strategy of DNA. Herein, highly bright DNA hairpin-templated silver nanoclusters (AgNCs) with quantum yield of 69.2% was firstly employed as the label-free signal output. The self-designed DNA hairpin was used as a molecular beacon by the relationship between the hairpin structure and the fluorescence signal of AgNCs. By recycling amplification of Exo-III, a significant change in fluorescence signal generated. Eventually, a label-free, visual, universal DNA detection platform based on Exo-III assisted amplification and DNA hairpin-AgNCs with high quantum yield was proposed.

KW - DNA detection

KW - Label-free detection

KW - Silver nanoclusters (AgNCs)

KW - Three-way junction

UR - https://www.scopus.com/pages/publications/85072148210

U2 - 10.1016/j.talanta.2019.120216

DO - 10.1016/j.talanta.2019.120216

M3 - Journal article

C2 - 31514829

AN - SCOPUS:85072148210

SN - 0039-9140

VL - 206

JO - Talanta

JF - Talanta

M1 - 120216

ER -

Three-way junction-promoted recycling amplification for sensitive DNA detection using highly bright DNA-silver nanocluster as label-free output

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