Three-way junction-promoted recycling amplification for sensitive DNA detection using highly bright DNA-silver nanocluster as label-free output

Fumiao Shen, He Qian, Yuliang Cheng, Yunfei Xie, Hang Yu, Weirong Yao, Renjun Pei, Y. Guo*, Hung Wing LI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

In this work, an intriguing phenomenon was found and studied that the efficiency of exonuclease (Exo) III-assisted recycling amplification could be greatly promoted by the three-way junction structure. This was utilized as a visualizable detection strategy of DNA. Herein, highly bright DNA hairpin-templated silver nanoclusters (AgNCs) with quantum yield of 69.2% was firstly employed as the label-free signal output. The self-designed DNA hairpin was used as a molecular beacon by the relationship between the hairpin structure and the fluorescence signal of AgNCs. By recycling amplification of Exo-III, a significant change in fluorescence signal generated. Eventually, a label-free, visual, universal DNA detection platform based on Exo-III assisted amplification and DNA hairpin-AgNCs with high quantum yield was proposed.

Original languageEnglish
Article number120216
JournalTalanta
Volume206
DOIs
Publication statusPublished - 1 Jan 2020

Scopus Subject Areas

  • Analytical Chemistry

User-Defined Keywords

  • DNA detection
  • Label-free detection
  • Silver nanoclusters (AgNCs)
  • Three-way junction

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