TY - JOUR
T1 - Three-way junction-promoted recycling amplification for sensitive DNA detection using highly bright DNA-silver nanocluster as label-free output
AU - Shen, Fumiao
AU - Qian, He
AU - Cheng, Yuliang
AU - Xie, Yunfei
AU - Yu, Hang
AU - Yao, Weirong
AU - Pei, Renjun
AU - Guo, Y.
AU - Li, Hung Wing
N1 - Funding Information:
This work was supported by the National Key Research and Development Program of China (No. 2017YFC1601704 , 2018YFC1604202 , 2018YFC1602300 ), the National Science Foundation of China (No. 31601552 ), the National First-class Discipline Program of Food Science and Technology ( JUFSTR20180509 ) and the “HongKong Scholar” program.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - In this work, an intriguing phenomenon was found and studied that the efficiency of exonuclease (Exo) III-assisted recycling amplification could be greatly promoted by the three-way junction structure. This was utilized as a visualizable detection strategy of DNA. Herein, highly bright DNA hairpin-templated silver nanoclusters (AgNCs) with quantum yield of 69.2% was firstly employed as the label-free signal output. The self-designed DNA hairpin was used as a molecular beacon by the relationship between the hairpin structure and the fluorescence signal of AgNCs. By recycling amplification of Exo-III, a significant change in fluorescence signal generated. Eventually, a label-free, visual, universal DNA detection platform based on Exo-III assisted amplification and DNA hairpin-AgNCs with high quantum yield was proposed.
AB - In this work, an intriguing phenomenon was found and studied that the efficiency of exonuclease (Exo) III-assisted recycling amplification could be greatly promoted by the three-way junction structure. This was utilized as a visualizable detection strategy of DNA. Herein, highly bright DNA hairpin-templated silver nanoclusters (AgNCs) with quantum yield of 69.2% was firstly employed as the label-free signal output. The self-designed DNA hairpin was used as a molecular beacon by the relationship between the hairpin structure and the fluorescence signal of AgNCs. By recycling amplification of Exo-III, a significant change in fluorescence signal generated. Eventually, a label-free, visual, universal DNA detection platform based on Exo-III assisted amplification and DNA hairpin-AgNCs with high quantum yield was proposed.
KW - DNA detection
KW - Label-free detection
KW - Silver nanoclusters (AgNCs)
KW - Three-way junction
UR - http://www.scopus.com/inward/record.url?scp=85072148210&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2019.120216
DO - 10.1016/j.talanta.2019.120216
M3 - Journal article
C2 - 31514829
AN - SCOPUS:85072148210
SN - 0039-9140
VL - 206
JO - Talanta
JF - Talanta
M1 - 120216
ER -