TY - JOUR
T1 - Therapeutic Potential of Pien Tze Huang on Experimental Autoimmune Encephalomyelitis Rat
AU - Qiu, Xuemei
AU - Luo, Hui
AU - Liu, Xue
AU - Guo, Qingqing
AU - Zheng, Kang
AU - Fan, Danping
AU - Shen, Jiawen
AU - Lu, Cheng
AU - He, Xiaojuan
AU - Zhang, Ge
AU - Lu, Aiping
N1 - Funding Information:
This research is supported by the Hong Kong Innovation and Technology Fund (UIM/310) and the Fundamental Research Funds for the Central Public Welfare Research Institutes (Z0551).
Publisher copyright:
Copyright © 2018 Xuemei Qiu et al.
PY - 2018/2/27
Y1 - 2018/2/27
N2 - Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS). There is still lack of commercially viable treatment currently. Pien Tze Huang (PZH), a traditional Chinese medicine, has been proved to have anti-inflammatory, neuroprotective, and immunoregulatory effects. This study investigated the possible therapeutic effects of PZH on experimental autoimmune encephalomyelitis (EAE) rats, a classic animal model of MS. Male Lewis rats were immunized with myelin basic protein (MBP) peptide to establish an EAE model and then treated with three doses of PZH. Clinical symptoms, organ coefficient, histopathological features, levels of proinflammatory cytokines, and chemokines as well as MBP and Olig2 were analyzed. The results indicated that PZH ameliorated the clinical severity of EAE rats. It also remarkably reduced inflammatory cell infiltration in the CNS of EAE rats. Furthermore, the levels of IL-17A, IL-23, CCL3, and CCL5 in serum and the CNS were significantly decreased; the p-P65 and p-STAT3 levels were also downregulated in the CNS, while MBP and Olig2 in the CNS of EAE rats had a distinct improvement after PZH treatment. In addition, PZH has no obvious toxicity at the concentration of 0.486 g/kg/d. This study demonstrated that PZH could be used to treat MS.
AB - Multiple sclerosis (MS) is a chronic inflammatory demyelinating disease of the central nervous system (CNS). There is still lack of commercially viable treatment currently. Pien Tze Huang (PZH), a traditional Chinese medicine, has been proved to have anti-inflammatory, neuroprotective, and immunoregulatory effects. This study investigated the possible therapeutic effects of PZH on experimental autoimmune encephalomyelitis (EAE) rats, a classic animal model of MS. Male Lewis rats were immunized with myelin basic protein (MBP) peptide to establish an EAE model and then treated with three doses of PZH. Clinical symptoms, organ coefficient, histopathological features, levels of proinflammatory cytokines, and chemokines as well as MBP and Olig2 were analyzed. The results indicated that PZH ameliorated the clinical severity of EAE rats. It also remarkably reduced inflammatory cell infiltration in the CNS of EAE rats. Furthermore, the levels of IL-17A, IL-23, CCL3, and CCL5 in serum and the CNS were significantly decreased; the p-P65 and p-STAT3 levels were also downregulated in the CNS, while MBP and Olig2 in the CNS of EAE rats had a distinct improvement after PZH treatment. In addition, PZH has no obvious toxicity at the concentration of 0.486 g/kg/d. This study demonstrated that PZH could be used to treat MS.
UR - http://www.scopus.com/inward/record.url?scp=85044043798&partnerID=8YFLogxK
U2 - 10.1155/2018/2952471
DO - 10.1155/2018/2952471
M3 - Journal article
C2 - 29682587
AN - SCOPUS:85044043798
SN - 2314-8861
VL - 2018
JO - Journal of Immunology Research
JF - Journal of Immunology Research
M1 - 2952471
ER -
