Therapeutic effects and mechanisms of Zhen-Wu-Bu-Qi Decoction on dextran sulfate sodium-induced chronic colitis in mice assessed by multi-omics approaches

Lixiang Zhai, Jiao Peng, Min Zhuang, Yao Yao Chang, Ka Wing Cheng, Zi Wan Ning, Tao Huang, Chengyuan Lin, Hoi Leong Xavier Wong, Yan Y. Lam, Hor Yue Tan, Hai Tao Xiao*, Zhao Xiang Bian*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Background

Zhen-Wu-Bu-Qi Decoction (ZWBQD), a traditional Chinese medicine formula comprising Poria, Radix Paeoniae Alba, Rhizoma Atractylodis Macrocephalae, Rhizoma Zingiberis Recens, Radix Codonopsis and Rhizoma Coptidis, is used for treating ulcerative colitis (UC). In a previous study, we have reported ZWBQD mitigates the severity of dextran sulfate sodium (DSS)-induced colitis in mice.

Hypothesis

In this study, we aimed to understand the systemic actions and underlying mechanisms of ZWBQD on experimental colitis in mice.

Methods

We used multi-omics techniques and immunoblotting approach to study the pharmacological actions and mechanisms of ZWBQD in DSS-induced chronic colitic mice.

Results

We showed that ZWBQD exhibited potent anti-inflammatory properties and significantly protected DSS-induced colitic mice against colon injury by regulating the PI3K-AKT, MAPK signaling pathway and NF-κB signaling pathways. We also revealed that ZWBQD significantly ameliorated gut microbiota dysbiosis and abnormalities of tryptophan catabolites induced by DSS.

Conclusions

We demonstrated that the therapeutic effects of ZWBQD on experimental colitis are mediated by regulating multiple signaling pathways and modulation of gut microbiota. Our study employed an integrative strategy to elucidate novel mechanisms of ZWBQD, which provides new insights into the development of Chinese herbal medicine-based therapeutics for UC.

Original languageEnglish
Article number154001
JournalPhytomedicine
Volume99
DOIs
Publication statusPublished - May 2022

Scopus Subject Areas

  • Drug Discovery
  • Molecular Medicine
  • Complementary and alternative medicine
  • Pharmacology
  • Pharmaceutical Science

User-Defined Keywords

  • Chinese medicine
  • Chronic colitis
  • Metabolomics
  • Metagenomics
  • System pharmacology

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