Therapeutic effects and mechanisms of Zhen-Wu-Bu-Qi Decoction on dextran sulfate sodium-induced chronic colitis in mice assessed by multi-omics approaches

Lixiang Zhai; Jiao Peng; Min Zhuang; Yao Yao  Chang; Ka Wing Cheng; Zi Wan  Ning; Tao Huang; Chengyuan  Lin; Hoi Leong Xavier Wong; Yan Y.  Lam; Hor Yue  Tan; Hai Tao  Xiao; Zhao Xiang  Bian

doi:10.1016/j.phymed.2022.154001

Therapeutic effects and mechanisms of Zhen-Wu-Bu-Qi Decoction on dextran sulfate sodium-induced chronic colitis in mice assessed by multi-omics approaches

Lixiang Zhai, Jiao Peng, Min Zhuang, Yao Yao Chang, Ka Wing Cheng, Zi Wan Ning, Tao Huang, Chengyuan Lin, Hoi Leong Xavier Wong, Yan Y. Lam, Hor Yue Tan, Hai Tao Xiao^*, Zhao Xiang Bian^*

^*Corresponding author for this work

Research output: Contribution to journal › Journal article › peer-review

13 Citations (Scopus)

Abstract

Background

Zhen-Wu-Bu-Qi Decoction (ZWBQD), a traditional Chinese medicine formula comprising Poria, Radix Paeoniae Alba, Rhizoma Atractylodis Macrocephalae, Rhizoma Zingiberis Recens, Radix Codonopsis and Rhizoma Coptidis, is used for treating ulcerative colitis (UC). In a previous study, we have reported ZWBQD mitigates the severity of dextran sulfate sodium (DSS)-induced colitis in mice.

Hypothesis

In this study, we aimed to understand the systemic actions and underlying mechanisms of ZWBQD on experimental colitis in mice.

Methods

We used multi-omics techniques and immunoblotting approach to study the pharmacological actions and mechanisms of ZWBQD in DSS-induced chronic colitic mice.

Results

We showed that ZWBQD exhibited potent anti-inflammatory properties and significantly protected DSS-induced colitic mice against colon injury by regulating the PI3K-AKT, MAPK signaling pathway and NF-κB signaling pathways. We also revealed that ZWBQD significantly ameliorated gut microbiota dysbiosis and abnormalities of tryptophan catabolites induced by DSS.

Conclusions

We demonstrated that the therapeutic effects of ZWBQD on experimental colitis are mediated by regulating multiple signaling pathways and modulation of gut microbiota. Our study employed an integrative strategy to elucidate novel mechanisms of ZWBQD, which provides new insights into the development of Chinese herbal medicine-based therapeutics for UC.

Original language	English
Article number	154001
Journal	Phytomedicine
Volume	99
DOIs	https://doi.org/10.1016/j.phymed.2022.154001
Publication status	Published - May 2022

Scopus Subject Areas

Drug Discovery
Molecular Medicine
Complementary and alternative medicine
Pharmacology
Pharmaceutical Science

User-Defined Keywords

Chinese medicine
Chronic colitis
Metabolomics
Metagenomics
System pharmacology

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10.1016/j.phymed.2022.154001

Cite this

Zhai, L., Peng, J., Zhuang, M., Chang, Y. Y., Cheng, K. W., Ning, Z. W., Huang, T., Lin, C., Wong, H. L. X., Lam, Y. Y., Tan, H. Y., Xiao, H. T., & Bian, Z. X. (2022). Therapeutic effects and mechanisms of Zhen-Wu-Bu-Qi Decoction on dextran sulfate sodium-induced chronic colitis in mice assessed by multi-omics approaches. Phytomedicine, 99, Article 154001. https://doi.org/10.1016/j.phymed.2022.154001

@article{1bcd0bb133b941ed8c91c36a65e36f14,

title = "Therapeutic effects and mechanisms of Zhen-Wu-Bu-Qi Decoction on dextran sulfate sodium-induced chronic colitis in mice assessed by multi-omics approaches",

abstract = "BackgroundZhen-Wu-Bu-Qi Decoction (ZWBQD), a traditional Chinese medicine formula comprising Poria, Radix Paeoniae Alba, Rhizoma Atractylodis Macrocephalae, Rhizoma Zingiberis Recens, Radix Codonopsis and Rhizoma Coptidis, is used for treating ulcerative colitis (UC). In a previous study, we have reported ZWBQD mitigates the severity of dextran sulfate sodium (DSS)-induced colitis in mice.HypothesisIn this study, we aimed to understand the systemic actions and underlying mechanisms of ZWBQD on experimental colitis in mice.MethodsWe used multi-omics techniques and immunoblotting approach to study the pharmacological actions and mechanisms of ZWBQD in DSS-induced chronic colitic mice.ResultsWe showed that ZWBQD exhibited potent anti-inflammatory properties and significantly protected DSS-induced colitic mice against colon injury by regulating the PI3K-AKT, MAPK signaling pathway and NF-κB signaling pathways. We also revealed that ZWBQD significantly ameliorated gut microbiota dysbiosis and abnormalities of tryptophan catabolites induced by DSS.ConclusionsWe demonstrated that the therapeutic effects of ZWBQD on experimental colitis are mediated by regulating multiple signaling pathways and modulation of gut microbiota. Our study employed an integrative strategy to elucidate novel mechanisms of ZWBQD, which provides new insights into the development of Chinese herbal medicine-based therapeutics for UC.",

keywords = "Chinese medicine, Chronic colitis, Metabolomics, Metagenomics, System pharmacology",

author = "Lixiang Zhai and Jiao Peng and Min Zhuang and Chang, {Yao Yao} and Cheng, {Ka Wing} and Ning, {Zi Wan} and Tao Huang and Chengyuan Lin and Wong, {Hoi Leong Xavier} and Lam, {Yan Y.} and Tan, {Hor Yue} and Xiao, {Hai Tao} and Bian, {Zhao Xiang}",

note = "Funding Information: This work was kindly funded by the Health@InnoHK Initiative Fund of the Hong Kong Special Administrative Region Government ( ITC RC/IHK/4/7 ), Key-Area Research and Development Program of Guangdong Province (No. 2020B1111110003), National Natural Science Foundation of China ( 81560676 and 81660479 ), Shenzhen Science and Technology Innovation Committee (No. JCYJ20210324093810026) and Medical Science and Technology Research Foundation of Guangdong province, China (No. A2020272). Publisher Copyright: {\textcopyright} 2022",

year = "2022",

month = may,

doi = "10.1016/j.phymed.2022.154001",

language = "English",

volume = "99",

journal = "Phytomedicine",

issn = "0944-7113",

publisher = "Elsevier",

}

TY - JOUR

T1 - Therapeutic effects and mechanisms of Zhen-Wu-Bu-Qi Decoction on dextran sulfate sodium-induced chronic colitis in mice assessed by multi-omics approaches

AU - Zhai, Lixiang

AU - Peng, Jiao

AU - Zhuang, Min

AU - Chang, Yao Yao

AU - Cheng, Ka Wing

AU - Ning, Zi Wan

AU - Huang, Tao

AU - Lin, Chengyuan

AU - Wong, Hoi Leong Xavier

AU - Lam, Yan Y.

AU - Tan, Hor Yue

AU - Xiao, Hai Tao

AU - Bian, Zhao Xiang

N1 - Funding Information: This work was kindly funded by the Health@InnoHK Initiative Fund of the Hong Kong Special Administrative Region Government ( ITC RC/IHK/4/7 ), Key-Area Research and Development Program of Guangdong Province (No. 2020B1111110003), National Natural Science Foundation of China ( 81560676 and 81660479 ), Shenzhen Science and Technology Innovation Committee (No. JCYJ20210324093810026) and Medical Science and Technology Research Foundation of Guangdong province, China (No. A2020272). Publisher Copyright: © 2022

PY - 2022/5

Y1 - 2022/5

N2 - BackgroundZhen-Wu-Bu-Qi Decoction (ZWBQD), a traditional Chinese medicine formula comprising Poria, Radix Paeoniae Alba, Rhizoma Atractylodis Macrocephalae, Rhizoma Zingiberis Recens, Radix Codonopsis and Rhizoma Coptidis, is used for treating ulcerative colitis (UC). In a previous study, we have reported ZWBQD mitigates the severity of dextran sulfate sodium (DSS)-induced colitis in mice.HypothesisIn this study, we aimed to understand the systemic actions and underlying mechanisms of ZWBQD on experimental colitis in mice.MethodsWe used multi-omics techniques and immunoblotting approach to study the pharmacological actions and mechanisms of ZWBQD in DSS-induced chronic colitic mice.ResultsWe showed that ZWBQD exhibited potent anti-inflammatory properties and significantly protected DSS-induced colitic mice against colon injury by regulating the PI3K-AKT, MAPK signaling pathway and NF-κB signaling pathways. We also revealed that ZWBQD significantly ameliorated gut microbiota dysbiosis and abnormalities of tryptophan catabolites induced by DSS.ConclusionsWe demonstrated that the therapeutic effects of ZWBQD on experimental colitis are mediated by regulating multiple signaling pathways and modulation of gut microbiota. Our study employed an integrative strategy to elucidate novel mechanisms of ZWBQD, which provides new insights into the development of Chinese herbal medicine-based therapeutics for UC.

AB - BackgroundZhen-Wu-Bu-Qi Decoction (ZWBQD), a traditional Chinese medicine formula comprising Poria, Radix Paeoniae Alba, Rhizoma Atractylodis Macrocephalae, Rhizoma Zingiberis Recens, Radix Codonopsis and Rhizoma Coptidis, is used for treating ulcerative colitis (UC). In a previous study, we have reported ZWBQD mitigates the severity of dextran sulfate sodium (DSS)-induced colitis in mice.HypothesisIn this study, we aimed to understand the systemic actions and underlying mechanisms of ZWBQD on experimental colitis in mice.MethodsWe used multi-omics techniques and immunoblotting approach to study the pharmacological actions and mechanisms of ZWBQD in DSS-induced chronic colitic mice.ResultsWe showed that ZWBQD exhibited potent anti-inflammatory properties and significantly protected DSS-induced colitic mice against colon injury by regulating the PI3K-AKT, MAPK signaling pathway and NF-κB signaling pathways. We also revealed that ZWBQD significantly ameliorated gut microbiota dysbiosis and abnormalities of tryptophan catabolites induced by DSS.ConclusionsWe demonstrated that the therapeutic effects of ZWBQD on experimental colitis are mediated by regulating multiple signaling pathways and modulation of gut microbiota. Our study employed an integrative strategy to elucidate novel mechanisms of ZWBQD, which provides new insights into the development of Chinese herbal medicine-based therapeutics for UC.

KW - Chinese medicine

KW - Chronic colitis

KW - Metabolomics

KW - Metagenomics

KW - System pharmacology

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U2 - 10.1016/j.phymed.2022.154001

DO - 10.1016/j.phymed.2022.154001

M3 - Journal article

C2 - 35240530

SN - 0944-7113

VL - 99

JO - Phytomedicine

JF - Phytomedicine

M1 - 154001

ER -

Therapeutic effects and mechanisms of Zhen-Wu-Bu-Qi Decoction on dextran sulfate sodium-induced chronic colitis in mice assessed by multi-omics approaches

Abstract

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