The use of cytokine array to examine cytokine profiles of two human cell lines exposed to indoor dust

Yuan Kang; Kwai Chung Cheung; Ming Hung WONG

doi:10.1016/j.toxlet.2010.09.012

The use of cytokine array to examine cytokine profiles of two human cell lines exposed to indoor dust

Yuan Kang, Kwai Chung Cheung, Ming Hung WONG^*

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

3 Citations (Scopus)

Abstract

Human cytokine array was used to investigate the cytokine profile of U937 and KERTr after exposure to indoor dust or dust extracts. The release of MCP-1 was increased while release of IL-8 and IL-1β on U937 were decreased after exposure to indoor dust. The releases of RANTES, IL-8 and VEGF from KERTr after exposure to dust extract were increased. The results of IL-8 ELISA assay were consistent with the cytokine array. Real-time RT-PCR was performed to analyze relative changes in gene expression. The MCP-1 mRNA levels were increased after U937 exposure to 18 indoor dust samples, whereas, IL-8 and IL-1β mRNA level showed both up-regulation and down-regulation. The dose-related increase and decrease response was observed on MCP-1 and IL-8, respectively. Most indoor dust extracts increased RANTES, IL-8 and VEGF mRNA levels on KERTr. The dose-dependent response was observed on RANTES and IL-8. A significant correlation (r= 0.48, p< 0.05) was obtained between the total PAHs concentration in dust extracts and the induction of RANTES mRNA.

Original language	English
Pages (from-to)	301-307
Number of pages	7
Journal	Toxicology Letters
Volume	199
Issue number	3
DOIs	https://doi.org/10.1016/j.toxlet.2010.09.012
Publication status	Published - 15 Dec 2010

Scopus Subject Areas

Toxicology

User-Defined Keywords

Human cytokine array
KERTr
U937

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10.1016/j.toxlet.2010.09.012

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title = "The use of cytokine array to examine cytokine profiles of two human cell lines exposed to indoor dust",

abstract = "Human cytokine array was used to investigate the cytokine profile of U937 and KERTr after exposure to indoor dust or dust extracts. The release of MCP-1 was increased while release of IL-8 and IL-1β on U937 were decreased after exposure to indoor dust. The releases of RANTES, IL-8 and VEGF from KERTr after exposure to dust extract were increased. The results of IL-8 ELISA assay were consistent with the cytokine array. Real-time RT-PCR was performed to analyze relative changes in gene expression. The MCP-1 mRNA levels were increased after U937 exposure to 18 indoor dust samples, whereas, IL-8 and IL-1β mRNA level showed both up-regulation and down-regulation. The dose-related increase and decrease response was observed on MCP-1 and IL-8, respectively. Most indoor dust extracts increased RANTES, IL-8 and VEGF mRNA levels on KERTr. The dose-dependent response was observed on RANTES and IL-8. A significant correlation (r= 0.48, p< 0.05) was obtained between the total PAHs concentration in dust extracts and the induction of RANTES mRNA.",

keywords = "Human cytokine array, KERTr, U937",

author = "Yuan Kang and Cheung, {Kwai Chung} and WONG, {Ming Hung}",

note = "Funding Information: Financial support from the Research Grants Council of the University Grants Committee of Hong Kong (HKBU 260209) and Special Equipment Grant (HKBU09) and the Mini-AoE (Area of Excellence, RC/AOE/08-09/01) Fund from Hong Kong Baptist University are gratefully acknowledged. ",

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PY - 2010/12/15

Y1 - 2010/12/15

N2 - Human cytokine array was used to investigate the cytokine profile of U937 and KERTr after exposure to indoor dust or dust extracts. The release of MCP-1 was increased while release of IL-8 and IL-1β on U937 were decreased after exposure to indoor dust. The releases of RANTES, IL-8 and VEGF from KERTr after exposure to dust extract were increased. The results of IL-8 ELISA assay were consistent with the cytokine array. Real-time RT-PCR was performed to analyze relative changes in gene expression. The MCP-1 mRNA levels were increased after U937 exposure to 18 indoor dust samples, whereas, IL-8 and IL-1β mRNA level showed both up-regulation and down-regulation. The dose-related increase and decrease response was observed on MCP-1 and IL-8, respectively. Most indoor dust extracts increased RANTES, IL-8 and VEGF mRNA levels on KERTr. The dose-dependent response was observed on RANTES and IL-8. A significant correlation (r= 0.48, p< 0.05) was obtained between the total PAHs concentration in dust extracts and the induction of RANTES mRNA.

AB - Human cytokine array was used to investigate the cytokine profile of U937 and KERTr after exposure to indoor dust or dust extracts. The release of MCP-1 was increased while release of IL-8 and IL-1β on U937 were decreased after exposure to indoor dust. The releases of RANTES, IL-8 and VEGF from KERTr after exposure to dust extract were increased. The results of IL-8 ELISA assay were consistent with the cytokine array. Real-time RT-PCR was performed to analyze relative changes in gene expression. The MCP-1 mRNA levels were increased after U937 exposure to 18 indoor dust samples, whereas, IL-8 and IL-1β mRNA level showed both up-regulation and down-regulation. The dose-related increase and decrease response was observed on MCP-1 and IL-8, respectively. Most indoor dust extracts increased RANTES, IL-8 and VEGF mRNA levels on KERTr. The dose-dependent response was observed on RANTES and IL-8. A significant correlation (r= 0.48, p< 0.05) was obtained between the total PAHs concentration in dust extracts and the induction of RANTES mRNA.

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The use of cytokine array to examine cytokine profiles of two human cell lines exposed to indoor dust

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