The Sperm Proteome of the Oyster Crassostrea hongkongensis

Huawei Mu, Shengwei Ke, Duo Zhang, Yanjie Zhang, Xiaoyuan Song, Ziniu Yu, Yang Zhang, Jianwen QIU*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Sperm proteins play vital roles in fertilization, but little is known about their identities in free-spawning marine invertebrates. Here, 286 sperm proteins are reported from the Hong Kong oyster Crassostrea hongkongensis using label-free and semi-quantitative proteomics. Proteins extracted from three sperm samples are separated by SDS-PAGE, analyzed by LC-MS/MS, and identified using Mascot. Functional classification of the sperm proteome reveals energy metabolism (33%), signaling and binding (23%), and protein synthesis and degradation (12%) as the top functional categories. Comparison of orthologous sperm proteins between C. hongkongensis, Crassostrea gigas, Mytilus edulis, and M. galloprovincialis suggests that energy metabolism (48%) is the most conserved functional group. Sequence alignment of the C. hongkongensis bindin, an acrosomal protein that binds the sperm and the egg, with those of three other Crassostrea species, reveals several conserved motifs. The study has enriched the data of invertebrate sperm proteins and may contribute to studies of mechanisms of fertilization in free-spawning invertebrates. The proteomic data are available in ProteomeXchange with the identifier PXD018255.

Original languageEnglish
Article number2000167
JournalProteomics
Volume20
Issue number19-20
DOIs
Publication statusPublished - 1 Oct 2020

Scopus Subject Areas

  • Biochemistry
  • Molecular Biology

User-Defined Keywords

  • bindin
  • Bivalvia
  • oyster
  • proteomics
  • sperm proteome

Fingerprint

Dive into the research topics of 'The Sperm Proteome of the Oyster Crassostrea hongkongensis'. Together they form a unique fingerprint.

Cite this