The sensitization of mice with a wild-type and cold-adapted variant of influenza A virus. II. Secondary cytotoxic T cell responses

Nai Ki Mak, C. Sweet, Gordon L. Ada, Gregory A. Tannock

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

Reductions in virus titers and the generation of enhanced cytotoxic T cell (Tc) activity in the lungs of mice primed either with a wild-type, parental (H2N2) influenza virus, A/AA/6/60, or a cold-adapted variant A/AA/6/60-ca and challenged 6 weeks later with a H1N1 A/WSN virus showed that both H2N2 viruses could sensitize the mice. A comparison of graded sensitizing doses of each virus showed that inocula of 106 tissue culture infective doses (TCID50) of the ca-variant or 103 TCID50 of the wild-type virus gave similar results. The spleens and lungs of normal mice were found to contain similar levels (circa 1/105 cells) of precursor Tc cells and the level in the lung did not increase 2 days after intranasal (i.n.) inoculation of A/WSN virus. Two and 6 weeks after priming mice with 105 TCID50 of either virus, the lungs contained a 20-fold increase in the precursor Tc cell frequency. In contrast, sensitization with a sub-lethal dose of mouse-adapted A/WSN virus caused a 100-fold or greater increase. Sensitization of mice with the parental but not the ca-variant virus caused an increase in frequency of precursor Tc cells in the spleens of the sensitized mice and this might reflect the very low level of replication of the ca-variant virus in the mouse lung.

Original languageEnglish
Pages (from-to)407-416
Number of pages10
JournalImmunology
Volume51
Issue number2
Publication statusPublished - Feb 1984

Scopus Subject Areas

  • Immunology and Allergy
  • Immunology

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