TY - JOUR
T1 - The retention behavior of ginsenosides in HPLC and its application to quality assessment of radix ginseng
AU - Hu, Ping
AU - Luo, Guo An
AU - Wang, Qing
AU - Zhao, Zhongzhen
AU - Wang, Wan
AU - Jiang, Zhi Hong
N1 - Funding Information:
This research was financially supported by the Research Grants Council, University Grants Committee of Hong Kong (HKBU 2130/04M), and the Scientific Research Plan Programme of the Shanghai Science and Technology Committee of China (05DZ19709).
PY - 2008/10
Y1 - 2008/10
N2 - This study systematically investigated the retention behavior of seven neutral ginsenosides Rg1, Re, Rf, Rb1, Rb2, Rc, Rd, and an acidic ginsenoside R0, the major pharmacologically active components of Radix Ginseng with RP-HPLC. The effects of solvent, pH value, ionic strength of the mobile phase, and column temperature were investigated using an octadecylsiloxane-bonded silica gel column. Based on the ginsenosides' retention characteristics, the concentration of acetonitrile and the gradient of the mobile phase needed to maintain the baseline separation of the major neutral ginsenosides in Radix Ginseng were theoretically predicted. Furthermore, the ionic strength of mobile-phase necessary to achieve good resolution of the neutral ginsenosides and acidic ginsenosides was carefully investigated. According to the results of the quantitative analysis of ginsenosides in eight batches of ginseng samples from different sources, the developed HPLC technique may be a valuable tool for the quality assessment of Radix Ginseng.
AB - This study systematically investigated the retention behavior of seven neutral ginsenosides Rg1, Re, Rf, Rb1, Rb2, Rc, Rd, and an acidic ginsenoside R0, the major pharmacologically active components of Radix Ginseng with RP-HPLC. The effects of solvent, pH value, ionic strength of the mobile phase, and column temperature were investigated using an octadecylsiloxane-bonded silica gel column. Based on the ginsenosides' retention characteristics, the concentration of acetonitrile and the gradient of the mobile phase needed to maintain the baseline separation of the major neutral ginsenosides in Radix Ginseng were theoretically predicted. Furthermore, the ionic strength of mobile-phase necessary to achieve good resolution of the neutral ginsenosides and acidic ginsenosides was carefully investigated. According to the results of the quantitative analysis of ginsenosides in eight batches of ginseng samples from different sources, the developed HPLC technique may be a valuable tool for the quality assessment of Radix Ginseng.
KW - Ginsenoside
KW - HPLC
KW - Panax
KW - Quantitation
KW - Radix Ginseng
KW - Retention behavior
UR - https://link.springer.com/article/10.1007/s12272-009-0005-z
UR - http://www.scopus.com/inward/record.url?scp=55149098184&partnerID=8YFLogxK
U2 - 10.1007/s12272-001-2105-2
DO - 10.1007/s12272-001-2105-2
M3 - Journal article
C2 - 19471880
AN - SCOPUS:55149098184
SN - 0253-6269
VL - 31
SP - 1265
EP - 1273
JO - Archives of Pharmacal Research
JF - Archives of Pharmacal Research
IS - 10
ER -