The profile of blood microbiome in new-onset type 1 diabetes children

Xiaoxiao Yuan; Xin Yang; Zhenran Xu; Jie Li; Cheng Jun Sun; Ruimin Chen; Haiyan Wei; Linqi Chen; Hongwei Du; Guimei Li; Yu Yang; Xiaojuan Chen; Lanwei Cui; Junfen Fu; Jin Wu; Zhihong Chen; Xin Fang; Zhe Su; Miaoying Zhang; Jing Wu; Xin Chen; Jiawei Zhou; Yue Luo; Lei Zhang; Ruirui Wang; Feihong Luo

doi:10.1016/j.isci.2024.110252

The profile of blood microbiome in new-onset type 1 diabetes children

Xiaoxiao Yuan, Xin Yang, Zhenran Xu, Jie Li, Cheng Jun Sun, Ruimin Chen, Haiyan Wei, Linqi Chen, Hongwei Du, Guimei Li, Yu Yang, Xiaojuan Chen, Lanwei Cui, Junfen Fu, Jin Wu, Zhihong Chen, Xin Fang, Zhe Su, Miaoying Zhang, Jing WuXin Chen, Jiawei Zhou, Yue Luo, Lei Zhang, Ruirui Wang^*, Feihong Luo^*

^*Corresponding author for this work

Chinese Medicine - Teaching and Research Division

Research output: Contribution to journal › Journal article › peer-review

3 Citations (Scopus)

Abstract

Blood microbiome signatures in patients with type 1 diabetes (T1D) remain unclear. We profile blood microbiome using 16S rRNA gene sequencing in 77 controls and 64 children with new-onset T1D, and compared it with the gut and oral microbiomes. The blood microbiome of patients with T1D is characterized by increased diversity and perturbed microbial features, with a significant increase in potentially pathogenic bacteria compared with controls. Thirty-six representative genera of blood microbiome were identified by random forest analysis, providing strong discriminatory power for T1D with an AUC of 0.82. PICRUSt analysis suggested that bacteria capable of inducing inflammation were more likely to enter the bloodstream in T1D. The overlap of the gut and oral microbiome with the blood microbiome implied potential translocation of bacteria from the gut and oral cavity to the bloodstream. Our study raised the necessity of further mechanistic investigations into the roles of blood microbiome in T1D.

Original language	English
Article number	110252
Number of pages	15
Journal	iScience
Volume	27
Issue number	7
DOIs	https://doi.org/10.1016/j.isci.2024.110252
Publication status	Published - 19 Jul 2024

User-Defined Keywords

Endocrine system physiology
Microbiome

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.isci.2024.110252

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@article{697a627d0c2342948329e3688ce13b98,

title = "The profile of blood microbiome in new-onset type 1 diabetes children",

abstract = "Blood microbiome signatures in patients with type 1 diabetes (T1D) remain unclear. We profile blood microbiome using 16S rRNA gene sequencing in 77 controls and 64 children with new-onset T1D, and compared it with the gut and oral microbiomes. The blood microbiome of patients with T1D is characterized by increased diversity and perturbed microbial features, with a significant increase in potentially pathogenic bacteria compared with controls. Thirty-six representative genera of blood microbiome were identified by random forest analysis, providing strong discriminatory power for T1D with an AUC of 0.82. PICRUSt analysis suggested that bacteria capable of inducing inflammation were more likely to enter the bloodstream in T1D. The overlap of the gut and oral microbiome with the blood microbiome implied potential translocation of bacteria from the gut and oral cavity to the bloodstream. Our study raised the necessity of further mechanistic investigations into the roles of blood microbiome in T1D.",

keywords = "Endocrine system physiology, Microbiome",

author = "Xiaoxiao Yuan and Xin Yang and Zhenran Xu and Jie Li and Sun, {Cheng Jun} and Ruimin Chen and Haiyan Wei and Linqi Chen and Hongwei Du and Guimei Li and Yu Yang and Xiaojuan Chen and Lanwei Cui and Junfen Fu and Jin Wu and Zhihong Chen and Xin Fang and Zhe Su and Miaoying Zhang and Jing Wu and Xin Chen and Jiawei Zhou and Yue Luo and Lei Zhang and Ruirui Wang and Feihong Luo",

note = "We thank the participants who made this study possible and provided the blood samples. This work was funded by the National Key Research and Development Program of China (2021YFC2701904 and 2016YFC1305302), the Clinical Special Project of Integrated Traditional Chinese and Western Medicine in 2019, Shanghai Muncipal Health Commission, Shanghai Muncipal Administration of Traditional Chinese Medicine (ZXYXZ-201902), Shanghai Collaborative Innovation Center for Chronic Disease Prevention and Health Services (2021 Science and Technology 02-37), and the Local College Faculty Constitution of Shanghai MSTC 2022 (22010504300). Publisher Copyright: {\textcopyright} 2024 The Authors",

year = "2024",

month = jul,

day = "19",

doi = "10.1016/j.isci.2024.110252",

language = "English",

volume = "27",

journal = "iScience",

issn = "2589-0042",

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number = "7",

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Yuan, X, Yang, X, Xu, Z, Li, J, Sun, CJ, Chen, R, Wei, H, Chen, L, Du, H, Li, G, Yang, Y, Chen, X, Cui, L, Fu, J, Wu, J, Chen, Z, Fang, X, Su, Z, Zhang, M, Wu, J, Chen, X, Zhou, J, Luo, Y, Zhang, L, Wang, R & Luo, F 2024, 'The profile of blood microbiome in new-onset type 1 diabetes children', iScience, vol. 27, no. 7, 110252. https://doi.org/10.1016/j.isci.2024.110252

TY - JOUR

T1 - The profile of blood microbiome in new-onset type 1 diabetes children

AU - Yuan, Xiaoxiao

AU - Yang, Xin

AU - Xu, Zhenran

AU - Li, Jie

AU - Sun, Cheng Jun

AU - Chen, Ruimin

AU - Wei, Haiyan

AU - Chen, Linqi

AU - Du, Hongwei

AU - Li, Guimei

AU - Yang, Yu

AU - Chen, Xiaojuan

AU - Cui, Lanwei

AU - Fu, Junfen

AU - Wu, Jin

AU - Chen, Zhihong

AU - Fang, Xin

AU - Su, Zhe

AU - Zhang, Miaoying

AU - Wu, Jing

AU - Chen, Xin

AU - Zhou, Jiawei

AU - Luo, Yue

AU - Zhang, Lei

AU - Wang, Ruirui

AU - Luo, Feihong

N1 - We thank the participants who made this study possible and provided the blood samples. This work was funded by the National Key Research and Development Program of China (2021YFC2701904 and 2016YFC1305302), the Clinical Special Project of Integrated Traditional Chinese and Western Medicine in 2019, Shanghai Muncipal Health Commission, Shanghai Muncipal Administration of Traditional Chinese Medicine (ZXYXZ-201902), Shanghai Collaborative Innovation Center for Chronic Disease Prevention and Health Services (2021 Science and Technology 02-37), and the Local College Faculty Constitution of Shanghai MSTC 2022 (22010504300). Publisher Copyright: © 2024 The Authors

PY - 2024/7/19

Y1 - 2024/7/19

N2 - Blood microbiome signatures in patients with type 1 diabetes (T1D) remain unclear. We profile blood microbiome using 16S rRNA gene sequencing in 77 controls and 64 children with new-onset T1D, and compared it with the gut and oral microbiomes. The blood microbiome of patients with T1D is characterized by increased diversity and perturbed microbial features, with a significant increase in potentially pathogenic bacteria compared with controls. Thirty-six representative genera of blood microbiome were identified by random forest analysis, providing strong discriminatory power for T1D with an AUC of 0.82. PICRUSt analysis suggested that bacteria capable of inducing inflammation were more likely to enter the bloodstream in T1D. The overlap of the gut and oral microbiome with the blood microbiome implied potential translocation of bacteria from the gut and oral cavity to the bloodstream. Our study raised the necessity of further mechanistic investigations into the roles of blood microbiome in T1D.

AB - Blood microbiome signatures in patients with type 1 diabetes (T1D) remain unclear. We profile blood microbiome using 16S rRNA gene sequencing in 77 controls and 64 children with new-onset T1D, and compared it with the gut and oral microbiomes. The blood microbiome of patients with T1D is characterized by increased diversity and perturbed microbial features, with a significant increase in potentially pathogenic bacteria compared with controls. Thirty-six representative genera of blood microbiome were identified by random forest analysis, providing strong discriminatory power for T1D with an AUC of 0.82. PICRUSt analysis suggested that bacteria capable of inducing inflammation were more likely to enter the bloodstream in T1D. The overlap of the gut and oral microbiome with the blood microbiome implied potential translocation of bacteria from the gut and oral cavity to the bloodstream. Our study raised the necessity of further mechanistic investigations into the roles of blood microbiome in T1D.

KW - Endocrine system physiology

KW - Microbiome

UR - http://www.scopus.com/inward/record.url?scp=85196615257&partnerID=8YFLogxK

U2 - 10.1016/j.isci.2024.110252

DO - 10.1016/j.isci.2024.110252

M3 - Journal article

AN - SCOPUS:85196615257

SN - 2589-0042

VL - 27

JO - iScience

JF - iScience

IS - 7

M1 - 110252

ER -

The profile of blood microbiome in new-onset type 1 diabetes children

Abstract

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