The preparation of poly-levodopa coated capillary column for capillary electrochromatography enantioseparation

Heying Guo, Yu Sun, Xiaoying Niu, Nannan Wei, Congjie Pan, Guoxiu Wang, Huige Zhang, Hongli Chen*, Tao Yi, Xingguo Chen

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

23 Citations (Scopus)


Levodopa (L-DOPA) is promising as chiral stationary phase for open tubular capillary electrochromatography (OT-CEC) enantioseparation owing to its self-polymerization adhesive property and chiral recognition potential. In this work, CuSO4/H2O2 was used as a trigger agent to accelerate the polymerization process of L-DOPA and the poly-levodopa (poly-(L-DOPA)) coated column was successfully prepared for the first time by depositing it on the inner wall of fused silica capillary via the rapid and in-situ approach at room temperature. The performance of the poly-(L-DOPA) coated capillary was validated by the separation of different chiral analytes, including chiral amine drugs, neurotransmitters and amino acids, and the good enantioseparation efficiencies were achieved. For five consecutive runs, the relative standard deviations (RSDs) for the migration time of the analytes for intra-day, inter-day and column-to-column were in the range of 0.19–1.33%, 0.96–4.47%, and 2.21–7.79%, respectively. Additionally, the poly-(L-DOPA) coated capillary column could be successively used over 250 runs without observable change in the separation efficiency.

Original languageEnglish
Pages (from-to)91-98
Number of pages8
JournalJournal of Chromatography A
Publication statusPublished - 30 Nov 2018

Scopus Subject Areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

User-Defined Keywords

  • Enantioseparation
  • Open tubular capillary electrochromatography
  • Poly-levodopa


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