The phosphoenolpyruvate/phosphate translocator is required for phenolic metabolism, palisade cell development, and plastid-dependent nuclear gene expression

Stephen J. Streatfield, Andreas Weber, Elizabeth A. Kinsman, Rainer E. Häusler, Jianming Li, Dusty Post-Beittenmiller, Werner M. Kaiser, Kevin A. Pyke, Ulf Ingo Flügge, Joanne Chory*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review

204 Citations (Scopus)

Abstract

The Arabidopsis chlorophyll a/b binding protein (CAB) gene underexpressed 1 (cue1) mutant underexpresses light-regulated nuclear genes encoding chloroplast-localized proteins. cue1 also exhibits mesophyll-specific chloroplast and cellular defects, resulting in reticulate leaves. Both the gene underexpression and the leaf cell morphology phenotypes are dependent on light intensity. In this study, we determine that CUE1 encodes the plastid inner envelope phosphoenolpyruvate/phosphate translocator (PPT) and define amino acid residues that are critical for translocator function. The biosynthesis of aromatics is compromised in cue1, and the reticulate phenotype can be rescued by feeding aromatic amino acids. Determining that CUE1 encodes PPT indicates the in vivo role of the translocator in metabolic partitioning and reveals a mesophyll cell-specific requirement for the translocator in Arabidopsis leaves. The nuclear gene expression defects in cue1 suggest that a light intensity-dependent interorganellar signal is modulated through metabolites dependent on a plastid supply of phosphoenolpyruvate.

Original languageEnglish
Pages (from-to)1609-1621
Number of pages13
JournalPlant Cell
Volume11
Issue number9
DOIs
Publication statusPublished - Sept 1999

Scopus Subject Areas

  • Plant Science

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