The pharmacokinetics analysis of the phosphoryl peptides in MCF-7/ADR cells

Peng Zhang, Mian Liu, Hongxia Liu, Zhenhua Xie, Feng Liu, Zongwei CAI, Yuyang Jiang*

*Corresponding author for this work

Research output: Contribution to journalJournal articlepeer-review


We have developed and validated a practical high-performance liquid chromatography method to determine the concentration of N- diisopropyloxyphosphoryl-valine-phenylalanine-3, 4, 5-trimethoxy aniline (DIPP-Val-Phe-TMOA) in Adriamycin-resistant human breast adenocarcinoma (MCF-7/ADR) cells. First, DIPP-Val-Phe-TMOA was extracted from MCF-7 cells with perchloric acid, followed by a cleanup procedure with centrifugal separation, and then determinated by HPLC using a Waters C18 column with a gradient elution (solvent A: 10 mM ammonium acetate at pH 7.0, B: acetonitrile). The flow rate was maintained at 1.0 ml/min at room temperature. The detection wavelength was at 254 nm. Meanwhile, this validated method was successfully applied to analyze the concentration levels and the pharmacokinetics parameters of DIPP-Val-Phe-TMOA in MCF-7/ADR cells.

Original languageEnglish
Pages (from-to)737-744
Number of pages8
JournalPhosphorus, Sulfur and Silicon and the Related Elements
Issue number2-3
Publication statusPublished - Feb 2008

Scopus Subject Areas

  • Biochemistry
  • Organic Chemistry
  • Inorganic Chemistry

User-Defined Keywords

  • Determination
  • High-performance liquid chromatography
  • MCF-7/ADR cells
  • N-diisopropyloxyphosphoryl-valine-phenylalanine-3,4,5-trimethoxyaniline


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