TY - JOUR
T1 - The pharmacokinetics analysis of the phosphoryl peptides in MCF-7/ADR cells
AU - Zhang, Peng
AU - Liu, Mian
AU - Liu, Hongxia
AU - Xie, Zhenhua
AU - Liu, Feng
AU - CAI, Zongwei
AU - Jiang, Yuyang
N1 - Funding Information:
Keywords Determination; high-performance liquid chromatography; MCF-7/ADR cells; N-diisopropyloxyphosphoryl-valine-phenylalanine-3,4,5-trimethoxyaniline This work was supported by the National Natural Science Foundation (China, 20472043, 20572060, 20672068), the Project of Science and Technology of Guangdong Province (2005A11601008), Mega-Project of Science Research of Ministry of Science and Technology (China, 2005CCA03400), and the Natural Science Foundation of Guangdong Province (China, 06028200).
PY - 2008/2
Y1 - 2008/2
N2 - We have developed and validated a practical high-performance liquid chromatography method to determine the concentration of N- diisopropyloxyphosphoryl-valine-phenylalanine-3, 4, 5-trimethoxy aniline (DIPP-Val-Phe-TMOA) in Adriamycin-resistant human breast adenocarcinoma (MCF-7/ADR) cells. First, DIPP-Val-Phe-TMOA was extracted from MCF-7 cells with perchloric acid, followed by a cleanup procedure with centrifugal separation, and then determinated by HPLC using a Waters C18 column with a gradient elution (solvent A: 10 mM ammonium acetate at pH 7.0, B: acetonitrile). The flow rate was maintained at 1.0 ml/min at room temperature. The detection wavelength was at 254 nm. Meanwhile, this validated method was successfully applied to analyze the concentration levels and the pharmacokinetics parameters of DIPP-Val-Phe-TMOA in MCF-7/ADR cells.
AB - We have developed and validated a practical high-performance liquid chromatography method to determine the concentration of N- diisopropyloxyphosphoryl-valine-phenylalanine-3, 4, 5-trimethoxy aniline (DIPP-Val-Phe-TMOA) in Adriamycin-resistant human breast adenocarcinoma (MCF-7/ADR) cells. First, DIPP-Val-Phe-TMOA was extracted from MCF-7 cells with perchloric acid, followed by a cleanup procedure with centrifugal separation, and then determinated by HPLC using a Waters C18 column with a gradient elution (solvent A: 10 mM ammonium acetate at pH 7.0, B: acetonitrile). The flow rate was maintained at 1.0 ml/min at room temperature. The detection wavelength was at 254 nm. Meanwhile, this validated method was successfully applied to analyze the concentration levels and the pharmacokinetics parameters of DIPP-Val-Phe-TMOA in MCF-7/ADR cells.
KW - Determination
KW - High-performance liquid chromatography
KW - MCF-7/ADR cells
KW - N-diisopropyloxyphosphoryl-valine-phenylalanine-3,4,5-trimethoxyaniline
UR - http://www.scopus.com/inward/record.url?scp=45849102426&partnerID=8YFLogxK
U2 - 10.1080/10426500701807749
DO - 10.1080/10426500701807749
M3 - Journal article
AN - SCOPUS:45849102426
SN - 1042-6507
VL - 183
SP - 737
EP - 744
JO - Phosphorus, Sulfur and Silicon and the Related Elements
JF - Phosphorus, Sulfur and Silicon and the Related Elements
IS - 2-3
ER -