TY - JOUR
T1 - The Morphology, Mitogenome, Phylogenetic Position, and Symbiotic Bacteria of a New Species of Sclerolinum (Annelida Siboglinidae) in the South China Sea
AU - Xu, Ting
AU - Sun, Yanan
AU - Wang, Zhi
AU - Sen, Arunima
AU - Qian, Pei Yuan
AU - Qiu, Jian Wen
N1 - Funding Information:
This project was supported by the National Key R&D Program, Ministry of Science and Technology, China (2018YFC0310005), the Key Special Project for Introduced Talents Team of Southern Marine Science and Engineering Guangdong Laboratory (Guangzhou) (GML2019ZD0409, SMSEGL20SC01, and SMSEGL20SC02), the Major of Basic and Applied Basic Research Project of Guangdong Province (2019B030302004), and the University Grants Committee of the Hong Kong Special Administrative Region (12101021).
Publisher Copyright:
Copyright © 2022 Xu, Sun, Wang, Sen, Qian and Qiu.
PY - 2022/2/8
Y1 - 2022/2/8
N2 - Sclerolinum annulatus n. sp. (Annelida: Siboglinidae) is described based on specimens collected from soft sediment of the Haima cold seep in the South China Sea. Morphologically, S. annulatus n. sp. is distinct in having a tube with transverse rings and a forepart (i.e., anterior region) containing one arched row of elongated plaques on both sides of the dorsal furrow. Genome skimming, assembly, and annotation produced a nearly complete mitogenome of S. annulatus n. sp. with 15,553 bp nucleotides that encodes 13 protein-coding genes, two rRNA, and 22 tRNA. Phylogenetic analyses based on the mitochondrial cytochrome c oxidase I (cox1) gene and a concatenated dataset comprising the mitochondrial cox1 and 16S rRNA genes along with the nuclear 18S rRNA gene both strongly support the placement of S. annulatus n. sp. in the genus Sclerolinum Southward, 1961. Based on cox1, S. annulatus n. sp. is most closely related to an undescribed siboglinid from off Kushiro in Japan (“Pogonophora” sp. Kushiro-SK-2003). Transmission electron microscopy, microbial 16S rRNA amplicon sequencing, phylogenetic reconstruction, and stable isotope analyses together indicate that S. annulatus n. sp. hosts a single phylotype of sulfur-oxidizing endosymbionts.
AB - Sclerolinum annulatus n. sp. (Annelida: Siboglinidae) is described based on specimens collected from soft sediment of the Haima cold seep in the South China Sea. Morphologically, S. annulatus n. sp. is distinct in having a tube with transverse rings and a forepart (i.e., anterior region) containing one arched row of elongated plaques on both sides of the dorsal furrow. Genome skimming, assembly, and annotation produced a nearly complete mitogenome of S. annulatus n. sp. with 15,553 bp nucleotides that encodes 13 protein-coding genes, two rRNA, and 22 tRNA. Phylogenetic analyses based on the mitochondrial cytochrome c oxidase I (cox1) gene and a concatenated dataset comprising the mitochondrial cox1 and 16S rRNA genes along with the nuclear 18S rRNA gene both strongly support the placement of S. annulatus n. sp. in the genus Sclerolinum Southward, 1961. Based on cox1, S. annulatus n. sp. is most closely related to an undescribed siboglinid from off Kushiro in Japan (“Pogonophora” sp. Kushiro-SK-2003). Transmission electron microscopy, microbial 16S rRNA amplicon sequencing, phylogenetic reconstruction, and stable isotope analyses together indicate that S. annulatus n. sp. hosts a single phylotype of sulfur-oxidizing endosymbionts.
KW - chemosynthesis
KW - deep-sea
KW - Haima cold seep
KW - siboglinid
KW - sulfur-oxidizing bacteria
UR - http://www.scopus.com/inward/record.url?scp=85125242752&partnerID=8YFLogxK
U2 - 10.3389/fmars.2021.793645
DO - 10.3389/fmars.2021.793645
M3 - Journal article
AN - SCOPUS:85125242752
SN - 2296-7745
VL - 8
JO - Frontiers in Marine Science
JF - Frontiers in Marine Science
M1 - 793645
ER -