The JAK2/STAT3 pathway is involved in the anti-melanoma effects of atractylenolide I

Xiuqiong Fu; Ji Yao Chou; Ting Li; Pei Li Zhu; Jun Kui Li; Cheng Le Yin; Tao Su; Hui Guo; Kin Wah Lee; Muhammad Jahangir Hossen; Gui Xin Chou; Zhiling Yu

doi:10.1111/exd.13454

The JAK2/STAT3 pathway is involved in the anti-melanoma effects of atractylenolide I

Xiuqiong Fu, Ji Yao Chou, Ting Li, Pei Li Zhu, Jun Kui Li, Cheng Le Yin, Tao Su, Hui Guo, Kin Wah Lee, Muhammad Jahangir Hossen, Gui Xin Chou^*, Zhiling Yu^*

^*Corresponding author for this work

Research output: Contribution to journal › Letter › peer-review

43 Citations (Scopus)

Abstract

In this study, we aimed to investigate the anti-melanoma effects and the JAK2/STAT3 pathway-related mechanism of action of atractylenolide I in human melanoma cells. Our results showed that atractylenolide I effectively reduced viability, induced apoptosis and inhibited migration of melanoma cells. Meanwhile, atractylenolide I decreased the protein expression levels of phospho-JAK2 and phospho-STAT3, and in turn downregulated the mRNA levels of STAT3-targeted genes, including Bcl-xL, MMP-2 and MMP-9. Furthermore, the cytotoxic effect of atractylenolide I was attenuated in STAT3-overactivated A375 cells. These findings indicate that inhibition of JAK2/STAT3 signalling contributes to the anti-melanoma effects of atractylenolide I.

Original language	English
Pages (from-to)	201-204
Number of pages	4
Journal	Experimental Dermatology
Volume	27
Issue number	2
Early online date	27 Oct 2017
DOIs	https://doi.org/10.1111/exd.13454
Publication status	Published - Feb 2018

User-Defined Keywords

apoptosis
Cytotoxicity
metastasis
molecular pathways
targeted therapy

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10.1111/exd.13454

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@article{c794daf8cf234155aae42039484640b1,

title = "The JAK2/STAT3 pathway is involved in the anti-melanoma effects of atractylenolide I",

abstract = "In this study, we aimed to investigate the anti-melanoma effects and the JAK2/STAT3 pathway-related mechanism of action of atractylenolide I in human melanoma cells. Our results showed that atractylenolide I effectively reduced viability, induced apoptosis and inhibited migration of melanoma cells. Meanwhile, atractylenolide I decreased the protein expression levels of phospho-JAK2 and phospho-STAT3, and in turn downregulated the mRNA levels of STAT3-targeted genes, including Bcl-xL, MMP-2 and MMP-9. Furthermore, the cytotoxic effect of atractylenolide I was attenuated in STAT3-overactivated A375 cells. These findings indicate that inhibition of JAK2/STAT3 signalling contributes to the anti-melanoma effects of atractylenolide I.",

keywords = "apoptosis, Cytotoxicity, metastasis, molecular pathways, targeted therapy",

author = "Xiuqiong Fu and Chou, {Ji Yao} and Ting Li and Zhu, {Pei Li} and Li, {Jun Kui} and Yin, {Cheng Le} and Tao Su and Hui Guo and Lee, {Kin Wah} and Hossen, {Muhammad Jahangir} and Chou, {Gui Xin} and Zhiling Yu",

note = "Funding Information: This study was supported by the grants from Research Grants Council of Hong Kong (GRF12125116); National Natural Science Foundation of China (81673649); Natural Science Foundation of Guangdong Province (2016A030313007); Science, Technology and Innovation Commission of Shenzhen (JCYJ20140807091945050, JCYJ20150630164505508 and JCYJ20160229210327924) and Hong Kong Baptist University (FRG1/16-17/048 and FRG2/16-17/033).",

year = "2018",

month = feb,

doi = "10.1111/exd.13454",

language = "English",

volume = "27",

pages = "201--204",

journal = "Experimental Dermatology",

issn = "0906-6705",

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T1 - The JAK2/STAT3 pathway is involved in the anti-melanoma effects of atractylenolide I

AU - Fu, Xiuqiong

AU - Chou, Ji Yao

AU - Li, Ting

AU - Zhu, Pei Li

AU - Li, Jun Kui

AU - Yin, Cheng Le

AU - Su, Tao

AU - Guo, Hui

AU - Lee, Kin Wah

AU - Hossen, Muhammad Jahangir

AU - Chou, Gui Xin

AU - Yu, Zhiling

N1 - Funding Information: This study was supported by the grants from Research Grants Council of Hong Kong (GRF12125116); National Natural Science Foundation of China (81673649); Natural Science Foundation of Guangdong Province (2016A030313007); Science, Technology and Innovation Commission of Shenzhen (JCYJ20140807091945050, JCYJ20150630164505508 and JCYJ20160229210327924) and Hong Kong Baptist University (FRG1/16-17/048 and FRG2/16-17/033).

PY - 2018/2

Y1 - 2018/2

N2 - In this study, we aimed to investigate the anti-melanoma effects and the JAK2/STAT3 pathway-related mechanism of action of atractylenolide I in human melanoma cells. Our results showed that atractylenolide I effectively reduced viability, induced apoptosis and inhibited migration of melanoma cells. Meanwhile, atractylenolide I decreased the protein expression levels of phospho-JAK2 and phospho-STAT3, and in turn downregulated the mRNA levels of STAT3-targeted genes, including Bcl-xL, MMP-2 and MMP-9. Furthermore, the cytotoxic effect of atractylenolide I was attenuated in STAT3-overactivated A375 cells. These findings indicate that inhibition of JAK2/STAT3 signalling contributes to the anti-melanoma effects of atractylenolide I.

AB - In this study, we aimed to investigate the anti-melanoma effects and the JAK2/STAT3 pathway-related mechanism of action of atractylenolide I in human melanoma cells. Our results showed that atractylenolide I effectively reduced viability, induced apoptosis and inhibited migration of melanoma cells. Meanwhile, atractylenolide I decreased the protein expression levels of phospho-JAK2 and phospho-STAT3, and in turn downregulated the mRNA levels of STAT3-targeted genes, including Bcl-xL, MMP-2 and MMP-9. Furthermore, the cytotoxic effect of atractylenolide I was attenuated in STAT3-overactivated A375 cells. These findings indicate that inhibition of JAK2/STAT3 signalling contributes to the anti-melanoma effects of atractylenolide I.

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KW - Cytotoxicity

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KW - molecular pathways

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DO - 10.1111/exd.13454

M3 - Letter

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JO - Experimental Dermatology

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The JAK2/STAT3 pathway is involved in the anti-melanoma effects of atractylenolide I

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