The involvement of regulatory non-coding RNAs in sepsis: A systematic review

Jeffery Ho; Hung Chan; Sunny H. Wong; Maggie Haitian Wang; Jun Yu; Zhangang Xiao; Xiaodong Liu; Gordon Choi; Czarina C.H. Leung; Wai T. Wong; Zheng Li; Tony Gin; Matthew T.V. Chan; William K.K. Wu

doi:10.1186/s13054-016-1555-3

The involvement of regulatory non-coding RNAs in sepsis: A systematic review

Jeffery Ho
, Hung Chan
, Sunny H. Wong^*
, Maggie Haitian Wang
, Jun Yu
, Zhangang Xiao
, Xiaodong Liu
, Gordon Choi
, Czarina C.H. Leung
, Wai T. Wong
, Zheng Li
, Tony Gin
, Matthew T.V. Chan^*
, William K.K. Wu

^*Corresponding author for this work

Research Centre for Herb Polysaccharides and Intestinal Immunology

Research output: Contribution to journal › Journal article › peer-review

87 Citations (Scopus)

Abstract

Background: Sepsis coincides with altered gene expression in different tissues. Accumulating evidence has suggested that microRNAs, long non-coding RNAs, and circular RNAs are important molecules involved in the crosstalk with various pathways pertinent to innate immunity, mitochondrial functions, and apoptosis. Methods: We searched articles indexed in PubMed (MEDLINE), EMBASE and Europe PubMed Central databases using the Medical Subject Heading (MeSH) or Title/Abstract words ("microRNA", "long non-coding RNA", "circular RNA", "sepsis" and/or "septic shock") from inception to Sep 2016. Studies investigating the role of host-derived microRNA, long non-coding RNA, and circular RNA in the pathogenesis of and as biomarkers or therapeutics in sepsis were included. Data were extracted in terms of the role of non-coding RNAs in pathogenesis, and their applicability for use as biomarkers or therapeutics in sepsis. Two independent researchers assessed the quality of studies using a modified guideline from the Systematic Review Center for Laboratory animal Experimentation (SYRCLE), a tool based on the Cochrane Collaboration Risk of Bias tool. Results: Observational studies revealed dysregulation of non-coding RNAs in septic patients. Experimental studies confirmed their crosstalk with JNK/NF-ΚB and other cellular pathways pertinent to innate immunity, mitochondrial function, and apoptosis. Of the included studies, the SYRCLE scores ranged from 3 to 7 (average score of 4.55). This suggests a moderate risk of bias. Of the 10 articles investigating non-coding RNAs as biomarkers, none of them included a validation cohort. Selective reporting of sensitivity, specificity, and receiver operating curve was common. Conclusions: Although non-coding RNAs appear to be good candidates as biomarkers and therapeutics for sepsis, their differential expression across tissues complicated the process. Further investigation on organ-specific delivery of these regulatory molecules may be useful.

Original language	English
Article number	383
Number of pages	12
Journal	Critical Care
Volume	20
Early online date	28 Nov 2016
DOIs	https://doi.org/10.1186/s13054-016-1555-3
Publication status	Published - Dec 2016

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

User-Defined Keywords

Biomarker
CircRNA
Inflammation
LncRNA
MicroRNA
Sepsis
Shock

Access to Document

10.1186/s13054-016-1555-3Licence: CC BY

Cite this

@article{a4fee4c8d5a747538c6f7e408abf93d4,

title = "The involvement of regulatory non-coding RNAs in sepsis: A systematic review",

abstract = "Background: Sepsis coincides with altered gene expression in different tissues. Accumulating evidence has suggested that microRNAs, long non-coding RNAs, and circular RNAs are important molecules involved in the crosstalk with various pathways pertinent to innate immunity, mitochondrial functions, and apoptosis. Methods: We searched articles indexed in PubMed (MEDLINE), EMBASE and Europe PubMed Central databases using the Medical Subject Heading (MeSH) or Title/Abstract words ({"}microRNA{"}, {"}long non-coding RNA{"}, {"}circular RNA{"}, {"}sepsis{"} and/or {"}septic shock{"}) from inception to Sep 2016. Studies investigating the role of host-derived microRNA, long non-coding RNA, and circular RNA in the pathogenesis of and as biomarkers or therapeutics in sepsis were included. Data were extracted in terms of the role of non-coding RNAs in pathogenesis, and their applicability for use as biomarkers or therapeutics in sepsis. Two independent researchers assessed the quality of studies using a modified guideline from the Systematic Review Center for Laboratory animal Experimentation (SYRCLE), a tool based on the Cochrane Collaboration Risk of Bias tool. Results: Observational studies revealed dysregulation of non-coding RNAs in septic patients. Experimental studies confirmed their crosstalk with JNK/NF-ΚB and other cellular pathways pertinent to innate immunity, mitochondrial function, and apoptosis. Of the included studies, the SYRCLE scores ranged from 3 to 7 (average score of 4.55). This suggests a moderate risk of bias. Of the 10 articles investigating non-coding RNAs as biomarkers, none of them included a validation cohort. Selective reporting of sensitivity, specificity, and receiver operating curve was common. Conclusions: Although non-coding RNAs appear to be good candidates as biomarkers and therapeutics for sepsis, their differential expression across tissues complicated the process. Further investigation on organ-specific delivery of these regulatory molecules may be useful.",

keywords = "Biomarker, CircRNA, Inflammation, LncRNA, MicroRNA, Sepsis, Shock",

author = "Jeffery Ho and Hung Chan and Wong, \{Sunny H.\} and Wang, \{Maggie Haitian\} and Jun Yu and Zhangang Xiao and Xiaodong Liu and Gordon Choi and Leung, \{Czarina C.H.\} and Wong, \{Wai T.\} and Zheng Li and Tony Gin and Chan, \{Matthew T.V.\} and Wu, \{William K.K.\}",

note = "This work was supported by the Hong Kong Research Grant Council-General Research Fund (464212, 24115815) and the Food and Health Bureau-Commissioned Research on Control of Infectious Diseases (CU-15-B2) and Health and Medical Research Fund (15140132). Publisher Copyright: {\textcopyright} 2016 The Author(s).",

year = "2016",

month = dec,

doi = "10.1186/s13054-016-1555-3",

language = "English",

volume = "20",

journal = "Critical Care",

issn = "1364-8535",

publisher = "BioMed Central Ltd.",

}

TY - JOUR

T1 - The involvement of regulatory non-coding RNAs in sepsis

T2 - A systematic review

AU - Ho, Jeffery

AU - Chan, Hung

AU - Wong, Sunny H.

AU - Wang, Maggie Haitian

AU - Yu, Jun

AU - Xiao, Zhangang

AU - Liu, Xiaodong

AU - Choi, Gordon

AU - Leung, Czarina C.H.

AU - Wong, Wai T.

AU - Li, Zheng

AU - Gin, Tony

AU - Chan, Matthew T.V.

AU - Wu, William K.K.

N1 - This work was supported by the Hong Kong Research Grant Council-General Research Fund (464212, 24115815) and the Food and Health Bureau-Commissioned Research on Control of Infectious Diseases (CU-15-B2) and Health and Medical Research Fund (15140132). Publisher Copyright: © 2016 The Author(s).

PY - 2016/12

Y1 - 2016/12

N2 - Background: Sepsis coincides with altered gene expression in different tissues. Accumulating evidence has suggested that microRNAs, long non-coding RNAs, and circular RNAs are important molecules involved in the crosstalk with various pathways pertinent to innate immunity, mitochondrial functions, and apoptosis. Methods: We searched articles indexed in PubMed (MEDLINE), EMBASE and Europe PubMed Central databases using the Medical Subject Heading (MeSH) or Title/Abstract words ("microRNA", "long non-coding RNA", "circular RNA", "sepsis" and/or "septic shock") from inception to Sep 2016. Studies investigating the role of host-derived microRNA, long non-coding RNA, and circular RNA in the pathogenesis of and as biomarkers or therapeutics in sepsis were included. Data were extracted in terms of the role of non-coding RNAs in pathogenesis, and their applicability for use as biomarkers or therapeutics in sepsis. Two independent researchers assessed the quality of studies using a modified guideline from the Systematic Review Center for Laboratory animal Experimentation (SYRCLE), a tool based on the Cochrane Collaboration Risk of Bias tool. Results: Observational studies revealed dysregulation of non-coding RNAs in septic patients. Experimental studies confirmed their crosstalk with JNK/NF-ΚB and other cellular pathways pertinent to innate immunity, mitochondrial function, and apoptosis. Of the included studies, the SYRCLE scores ranged from 3 to 7 (average score of 4.55). This suggests a moderate risk of bias. Of the 10 articles investigating non-coding RNAs as biomarkers, none of them included a validation cohort. Selective reporting of sensitivity, specificity, and receiver operating curve was common. Conclusions: Although non-coding RNAs appear to be good candidates as biomarkers and therapeutics for sepsis, their differential expression across tissues complicated the process. Further investigation on organ-specific delivery of these regulatory molecules may be useful.

AB - Background: Sepsis coincides with altered gene expression in different tissues. Accumulating evidence has suggested that microRNAs, long non-coding RNAs, and circular RNAs are important molecules involved in the crosstalk with various pathways pertinent to innate immunity, mitochondrial functions, and apoptosis. Methods: We searched articles indexed in PubMed (MEDLINE), EMBASE and Europe PubMed Central databases using the Medical Subject Heading (MeSH) or Title/Abstract words ("microRNA", "long non-coding RNA", "circular RNA", "sepsis" and/or "septic shock") from inception to Sep 2016. Studies investigating the role of host-derived microRNA, long non-coding RNA, and circular RNA in the pathogenesis of and as biomarkers or therapeutics in sepsis were included. Data were extracted in terms of the role of non-coding RNAs in pathogenesis, and their applicability for use as biomarkers or therapeutics in sepsis. Two independent researchers assessed the quality of studies using a modified guideline from the Systematic Review Center for Laboratory animal Experimentation (SYRCLE), a tool based on the Cochrane Collaboration Risk of Bias tool. Results: Observational studies revealed dysregulation of non-coding RNAs in septic patients. Experimental studies confirmed their crosstalk with JNK/NF-ΚB and other cellular pathways pertinent to innate immunity, mitochondrial function, and apoptosis. Of the included studies, the SYRCLE scores ranged from 3 to 7 (average score of 4.55). This suggests a moderate risk of bias. Of the 10 articles investigating non-coding RNAs as biomarkers, none of them included a validation cohort. Selective reporting of sensitivity, specificity, and receiver operating curve was common. Conclusions: Although non-coding RNAs appear to be good candidates as biomarkers and therapeutics for sepsis, their differential expression across tissues complicated the process. Further investigation on organ-specific delivery of these regulatory molecules may be useful.

KW - Biomarker

KW - CircRNA

KW - Inflammation

KW - LncRNA

KW - MicroRNA

KW - Sepsis

KW - Shock

UR - https://www.scopus.com/pages/publications/84999280649

UR - https://link.springer.com/article/10.1186/s13054-016-1555-3#Abs1

U2 - 10.1186/s13054-016-1555-3

DO - 10.1186/s13054-016-1555-3

M3 - Journal article

C2 - 27890015

AN - SCOPUS:84999280649

SN - 1364-8535

VL - 20

JO - Critical Care

JF - Critical Care

M1 - 383

ER -

The involvement of regulatory non-coding RNAs in sepsis: A systematic review

Abstract

UN SDGs

User-Defined Keywords

Access to Document

Other files and links

Fingerprint

Cite this