The ginsenoside protopanaxatriol protects endothelial cells from hydrogen peroxide-induced cell injury and cell death by modulating intracellular redox status

Hoi Hin Kwok, Wai Yee Ng, Mildred Sze Ming Yang, Nai Ki MAK, Ricky N S WONG, Patrick Y K YUE*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Citations (Scopus)

Abstract

Ginsenosides, the active components of the famous Chinese herb ginseng, have been suggested to possess cardiovascular-protective effects. The mechanism of ginsenosides is believed to be associated with their ability to prevent cellular oxidative stress. The purpose of this study was to explore the cytoprotective effects of the ginsenoside protopanaxatriol (PPT) on hydrogen peroxide (H2O2)-induced endothelial cell injury and cell death. Pretreatment of human umbilical vein endothelial cells (HUVECs) with PPT for 24 h was able to protect the cells against H2O2-induced injury. In addition to cell death, pretreatment with PPT could also reduce H2O2-induced DNA damage, overactivation of the DNA repair enzyme PARP-1, and concomitant depletion of the intracellular substrate NAD+. Furthermore, PPT could reverse the decrease in ATP/ADP ratio caused by H2O2. The metabolism of glutathione was also changed. H2O2 could induce a significant decrease in GSH level resulting in a decrease in the GSH/GSSG ratio. This could be prevented by pretreatment with PPT. The action was associated with increasing activities of the GSH-metabolizing enzymes glutathione reductase and glutathione peroxidase. These findings suggest that the ginsenoside PPT could protect HUVECs against H2O2-induced cell death via its action against oxidative stress, which may be responsible for the cardiovascular-protective action of ginseng.

Original languageEnglish
Pages (from-to)437-445
Number of pages9
JournalFree Radical Biology and Medicine
Volume48
Issue number3
DOIs
Publication statusPublished - 1 Feb 2010

Scopus Subject Areas

  • Biochemistry
  • Physiology (medical)

User-Defined Keywords

  • Cellular redox status
  • Endothelial cell
  • Free radicals
  • Ginsenoside
  • Hydrogen peroxide
  • Oxidative stress
  • Protopanaxatriol

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