The effects of methimazole on haematopoiesis in mice

N. K. Mak; W. H. Lai; W. K. Liu; K. N. Leung; M. C. Fung

doi:10.3109/08923979509037193

The effects of methimazole on haematopoiesis in mice

N. K. Mak^*, W. H. Lai, W. K. Liu, K. N. Leung, M. C. Fung

^*Corresponding author for this work

Department of Biology

Research output: Contribution to journal › Journal article › peer-review

3 Citations (Scopus)

Abstract

The effects of anti-thyroid drug, methimazole (MMI), on haematopoiesis in inbred C57BL/6 mice were studied. The in vitro proliferative response of bone marrow (BM) cells to interleukin-3 (IL-3) was significantly increased when mice were provided with 0.1% MMI in water (w/v) ad librium for 4 to 6 weeks. Using soft agar agar colony assay, the numbers of myeloid cell colonies were also significantly increased in mice treated with MMI. However, the proliferative response of BM cells to IL-3 was found to be greatly reduced 10 weeks after MMI treatment. In vitro studies showed that MMI alone at the concentrations of 500μM or above inhibited both the growth of normal BM cells in liquid culture and the formation of macrophage (M) - / granulocyte (G) - colonies in soft agar culture in a dose dependent manner. Direct cytotoxic effect of MMI (0 - 1250 μM) to normal BM cells was not observed. Results from this study suggested that MMI can modulate the development of myeloid haematopoietic cells.

Original language	English
Pages (from-to)	745-757
Number of pages	13
Journal	Immunopharmacology and Immunotoxicology
Volume	17
Issue number	4
DOIs	https://doi.org/10.3109/08923979509037193
Publication status	Published - 1995

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title = "The effects of methimazole on haematopoiesis in mice",

abstract = "The effects of anti-thyroid drug, methimazole (MMI), on haematopoiesis in inbred C57BL/6 mice were studied. The in vitro proliferative response of bone marrow (BM) cells to interleukin-3 (IL-3) was significantly increased when mice were provided with 0.1% MMI in water (w/v) ad librium for 4 to 6 weeks. Using soft agar agar colony assay, the numbers of myeloid cell colonies were also significantly increased in mice treated with MMI. However, the proliferative response of BM cells to IL-3 was found to be greatly reduced 10 weeks after MMI treatment. In vitro studies showed that MMI alone at the concentrations of 500μM or above inhibited both the growth of normal BM cells in liquid culture and the formation of macrophage (M) - / granulocyte (G) - colonies in soft agar culture in a dose dependent manner. Direct cytotoxic effect of MMI (0 - 1250 μM) to normal BM cells was not observed. Results from this study suggested that MMI can modulate the development of myeloid haematopoietic cells.",

author = "Mak, {N. K.} and Lai, {W. H.} and Liu, {W. K.} and Leung, {K. N.} and Fung, {M. C.}",

year = "1995",

doi = "10.3109/08923979509037193",

language = "English",

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pages = "745--757",

journal = "Immunopharmacology and Immunotoxicology",

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T1 - The effects of methimazole on haematopoiesis in mice

AU - Mak, N. K.

AU - Lai, W. H.

AU - Liu, W. K.

AU - Leung, K. N.

AU - Fung, M. C.

PY - 1995

Y1 - 1995

N2 - The effects of anti-thyroid drug, methimazole (MMI), on haematopoiesis in inbred C57BL/6 mice were studied. The in vitro proliferative response of bone marrow (BM) cells to interleukin-3 (IL-3) was significantly increased when mice were provided with 0.1% MMI in water (w/v) ad librium for 4 to 6 weeks. Using soft agar agar colony assay, the numbers of myeloid cell colonies were also significantly increased in mice treated with MMI. However, the proliferative response of BM cells to IL-3 was found to be greatly reduced 10 weeks after MMI treatment. In vitro studies showed that MMI alone at the concentrations of 500μM or above inhibited both the growth of normal BM cells in liquid culture and the formation of macrophage (M) - / granulocyte (G) - colonies in soft agar culture in a dose dependent manner. Direct cytotoxic effect of MMI (0 - 1250 μM) to normal BM cells was not observed. Results from this study suggested that MMI can modulate the development of myeloid haematopoietic cells.

AB - The effects of anti-thyroid drug, methimazole (MMI), on haematopoiesis in inbred C57BL/6 mice were studied. The in vitro proliferative response of bone marrow (BM) cells to interleukin-3 (IL-3) was significantly increased when mice were provided with 0.1% MMI in water (w/v) ad librium for 4 to 6 weeks. Using soft agar agar colony assay, the numbers of myeloid cell colonies were also significantly increased in mice treated with MMI. However, the proliferative response of BM cells to IL-3 was found to be greatly reduced 10 weeks after MMI treatment. In vitro studies showed that MMI alone at the concentrations of 500μM or above inhibited both the growth of normal BM cells in liquid culture and the formation of macrophage (M) - / granulocyte (G) - colonies in soft agar culture in a dose dependent manner. Direct cytotoxic effect of MMI (0 - 1250 μM) to normal BM cells was not observed. Results from this study suggested that MMI can modulate the development of myeloid haematopoietic cells.

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U2 - 10.3109/08923979509037193

DO - 10.3109/08923979509037193

M3 - Journal article

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AN - SCOPUS:0028867682

SN - 0892-3973

VL - 17

SP - 745

EP - 757

JO - Immunopharmacology and Immunotoxicology

JF - Immunopharmacology and Immunotoxicology

IS - 4

ER -

The effects of methimazole on haematopoiesis in mice

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