The detection of melamine base on a turn-on fluorescence of DNA-Ag nanoclusters

Peisi Xie, Yuanjin Zhan, Mei Wu, Longhua Guo, Zhenyu Lin, Bin Qiu*, Guonan Chen, Zongwei CAI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

A label-free, ultra-sensitive and turn-on fluorescence method to detect melamine has been developed. The strategy uses the DNA-Ag nanoclusters (DNA-AgNCs) as the fluorescence probe. The fluorescence of DNA-AgNCs can be quenched in presence of Hg2+. However, When Hg2+ and melamine were reacted for 15 min at 1000 rmp then introduced into DNA-AgNCs solution, a strong fluorescence recovery could be found. Based on this methodology, the changing fluorescent intensity has a linear relationship with melamine in the range of 0.2 μM to 4 μM (R2=0.997). The detection limit down to 0.1 μM was obtained, which is 200 times lower than the melamine safety limit of 20 μM estimated by the US Food and Drug Administration. In addition, we had been successfully applied this method to detect melamine in milk powder and raw milk.

Original languageEnglish
Pages (from-to)103-108
Number of pages6
JournalJournal of Luminescence
Volume186
DOIs
Publication statusPublished - 1 Jun 2017

Scopus Subject Areas

  • Biophysics
  • Biochemistry
  • Chemistry(all)
  • Atomic and Molecular Physics, and Optics
  • Condensed Matter Physics

User-Defined Keywords

  • DNA-Ag nanoclusters
  • Hg
  • melamine

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