TY - JOUR
T1 - The changing HIV-1 genetic characteristics and transmitted drug resistance among recently infected population in Yunnan, China
AU - Chen, M.
AU - Jia, M. H.
AU - Ma, Y. L.
AU - Luo, H. B.
AU - Chen, H. C.
AU - Yang, C. J.
AU - Dai, J.
AU - Yang, L.
AU - Dong, L. J.
AU - Lu, R.
AU - Song, L. J.
AU - Han, Y.
AU - Lu, J. Y.
AU - Cheung, Ka Loon Allen
AU - Chen, Z. W.
AU - Lu, L.
N1 - Funding Information:
This work was supported by the National Natural Science Foundation of China (grant no. 81560327), the research project for Yunnan science and technology plan (grant no. 2015FB200), Yunnan health science and technology plan project (grant no. 2014NS348) and the Major Project of China's 'Twelfth Five-Year Plan' for Science and Technology Development (2013ZX10004-906).
PY - 2018/4/1
Y1 - 2018/4/1
N2 - Multiple human immunodeficiency virus (HIV)-1 genotypes in China were first discovered in Yunnan Province before disseminating throughout the country. As the HIV-1 epidemic continues to expand in Yunnan, genetic characteristics and transmitted drug resistance (TDR) should be further investigated among the recently infected population. Among 2828 HIV-positive samples newly reported in the first quarter of 2014, 347 were identified as recent infections with BED-captured enzyme immunoassay (CEIA). Of them, 291 were successfully genotyped and identified as circulating recombinant form (CRF)08-BC (47.4%), unique recombinant forms (URFs) (18.2%), CRF01-AE (15.8%), CRF07-BC (14.4%), subtype C (2.7%), CRF55-01B (0.7%), subtype B (0.3%) and CRF64-BC (0.3%). CRF08-BC and CRF01-AE were the predominant genotypes among heterosexual and homosexual infections, respectively. CRF08-BC, URFs, CRF01-AE and CRF07-BC expanded with higher prevalence in central and eastern Yunnan. The recent common ancestor of CRF01-AE, CRF07-BC and CRF08-BC dated back to 1983.1, 1992.1 and 1989.5, respectively. The effective population sizes (EPS) for CRF01-AE and CRF07-BC increased exponentially during 1991-1999 and 1994-1999, respectively. The EPS for CRF08-BC underwent two exponential growth phases in 1994-1998 and 2001-2002. Lastly, TDR-associated mutations were identified in 1.8% of individuals. These findings not only enhance our understanding of HIV-1 evolution in Yunnan but also have implications for vaccine design and patient management strategies.
AB - Multiple human immunodeficiency virus (HIV)-1 genotypes in China were first discovered in Yunnan Province before disseminating throughout the country. As the HIV-1 epidemic continues to expand in Yunnan, genetic characteristics and transmitted drug resistance (TDR) should be further investigated among the recently infected population. Among 2828 HIV-positive samples newly reported in the first quarter of 2014, 347 were identified as recent infections with BED-captured enzyme immunoassay (CEIA). Of them, 291 were successfully genotyped and identified as circulating recombinant form (CRF)08-BC (47.4%), unique recombinant forms (URFs) (18.2%), CRF01-AE (15.8%), CRF07-BC (14.4%), subtype C (2.7%), CRF55-01B (0.7%), subtype B (0.3%) and CRF64-BC (0.3%). CRF08-BC and CRF01-AE were the predominant genotypes among heterosexual and homosexual infections, respectively. CRF08-BC, URFs, CRF01-AE and CRF07-BC expanded with higher prevalence in central and eastern Yunnan. The recent common ancestor of CRF01-AE, CRF07-BC and CRF08-BC dated back to 1983.1, 1992.1 and 1989.5, respectively. The effective population sizes (EPS) for CRF01-AE and CRF07-BC increased exponentially during 1991-1999 and 1994-1999, respectively. The EPS for CRF08-BC underwent two exponential growth phases in 1994-1998 and 2001-2002. Lastly, TDR-associated mutations were identified in 1.8% of individuals. These findings not only enhance our understanding of HIV-1 evolution in Yunnan but also have implications for vaccine design and patient management strategies.
KW - China
KW - HIV-1
KW - molecular epidemiology
KW - transmitted drug resistance
KW - Yunnan
UR - http://www.scopus.com/inward/record.url?scp=85043679598&partnerID=8YFLogxK
U2 - 10.1017/S0950268818000389
DO - 10.1017/S0950268818000389
M3 - Journal article
C2 - 29534773
AN - SCOPUS:85043679598
SN - 0950-2688
VL - 146
SP - 775
EP - 781
JO - Epidemiology and Infection
JF - Epidemiology and Infection
IS - 6
ER -