TY - JOUR
T1 - The cellular effects of PM2.5 collected in Chinese Taiyuan and Guangzhou and their associations with polycyclic aromatic hydrocarbons (PAHs), nitro-PAHs and hydroxy-PAHs
AU - Song, Yuanyuan
AU - Zhang, Yanhao
AU - Li, Ruijin
AU - Chen, Wei
AU - Chung, Chi Kong Arthur
AU - CAI, Zongwei
N1 - Funding Information:
This work was supported by National Natural Science Foundation of China (91543202 and 91843301) and Collaborative Research Fund (C2014-14 E) from Research Grants Council of Hong Kong. The authors also would like to thank Prof. Jianzhen Yu in Hong Kong University of Science of Technology for providing aerosol carbon analyzer. Dr Simon Wang at the Language Centre of HKBU has helped improve the linguistic presentation of the manuscript.
Funding Information:
This work was supported by National Natural Science Foundation of China ( 91543202 and 91843301 ) and Collaborative Research Fund ( C2014-14 E ) from Research Grants Council of Hong Kong. The authors also would like to thank Prof. Jianzhen Yu in Hong Kong University of Science of Technology for providing aerosol carbon analyzer. Dr Simon Wang at the Language Centre of HKBU has helped improve the linguistic presentation of the manuscript.
PY - 2020/3/15
Y1 - 2020/3/15
N2 - Numerous studies have demonstrated adverse effects on human health after exposure to fine particulate matter (PM2.5). However, it is still not clear how the toxicological effects and the health risks vary among PM samples of different compositions and concentrations. In this study, we examined effects of region- and season-dependent differences of PM2.5 on cytotoxicity, and the contributions of PAHs, nitro-PAHs (N-PAHs) and hydroxy-PAHs (OH-PAHs) to PM2.5 toxicity by determining different toxicological indicators in three lung cell lines. The results illustrated significant differences in components concentrations and biological responses elicited by PM2.5 collected in different cities and seasons. The concentrations of most PAHs, N-PAHs and OH-PAHs were much higher in Taiyuan than in Guangzhou. PM2.5 from Taiyuan exhibited lower cell viability and higher reactive oxygen species (ROS) and interleukin-6 (IL-6) release on lung cells than those from Guangzhou. Specifically, PM2.5 collected in summer from Taiyuan caused higher levels of pro-inflammatory responses and oxidative potential than those collected in winter. The correlation analysis between 19 PAHs, 17 N-PAHs and 12 OH-PAHs and the measured indicators demonstrated that PAHs were more related to PM2.5-induced CCK-8 cytotoxicity and IL-6 release in Taiyuan while N-PAHs and OH-PAHs were more related to PM2.5-induced CCK-8 cytotoxicity and dithiothreitol (DTT)-based redox activity in Guangzhou, suggesting that the toxicity of PM2.5 from Taiyuan was mostly correlated with PAHs while the toxicity of PM2.5 from Guangzhou was closely associated with N-PAHs and OH-PAHs. These results revealed that composition differences in PM2.5 from different regions and seasons significantly accounted for the differences of their toxicological effects.
AB - Numerous studies have demonstrated adverse effects on human health after exposure to fine particulate matter (PM2.5). However, it is still not clear how the toxicological effects and the health risks vary among PM samples of different compositions and concentrations. In this study, we examined effects of region- and season-dependent differences of PM2.5 on cytotoxicity, and the contributions of PAHs, nitro-PAHs (N-PAHs) and hydroxy-PAHs (OH-PAHs) to PM2.5 toxicity by determining different toxicological indicators in three lung cell lines. The results illustrated significant differences in components concentrations and biological responses elicited by PM2.5 collected in different cities and seasons. The concentrations of most PAHs, N-PAHs and OH-PAHs were much higher in Taiyuan than in Guangzhou. PM2.5 from Taiyuan exhibited lower cell viability and higher reactive oxygen species (ROS) and interleukin-6 (IL-6) release on lung cells than those from Guangzhou. Specifically, PM2.5 collected in summer from Taiyuan caused higher levels of pro-inflammatory responses and oxidative potential than those collected in winter. The correlation analysis between 19 PAHs, 17 N-PAHs and 12 OH-PAHs and the measured indicators demonstrated that PAHs were more related to PM2.5-induced CCK-8 cytotoxicity and IL-6 release in Taiyuan while N-PAHs and OH-PAHs were more related to PM2.5-induced CCK-8 cytotoxicity and dithiothreitol (DTT)-based redox activity in Guangzhou, suggesting that the toxicity of PM2.5 from Taiyuan was mostly correlated with PAHs while the toxicity of PM2.5 from Guangzhou was closely associated with N-PAHs and OH-PAHs. These results revealed that composition differences in PM2.5 from different regions and seasons significantly accounted for the differences of their toxicological effects.
KW - Correlation analysis
KW - PAHs and their derivatives
KW - Physicochemical characteristics
KW - PM
KW - Toxicological indicators
UR - http://www.scopus.com/inward/record.url?scp=85078186404&partnerID=8YFLogxK
U2 - 10.1016/j.ecoenv.2020.110225
DO - 10.1016/j.ecoenv.2020.110225
M3 - Journal article
C2 - 32001423
AN - SCOPUS:85078186404
SN - 0147-6513
VL - 191
JO - Ecotoxicology and Environmental Safety
JF - Ecotoxicology and Environmental Safety
M1 - 110225
ER -
