The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

Lifeng Peng; Eugene A. Kapp; David Fenyö; Min Seok Kwon; Pu Jiang; Songfeng Wu; Ying Jiang; Marie Isabel Aguilar; Nikhat Ahmed; Mark S. Baker; Zongwei CAI; Yu Ju Chen; Phan Van Chi; Maxey C.M. Chung; Fuchu He; Alice C.L. Len; Pao Chi Liao; Kazuyuki Nakamura; Sai Ming Ngai; Young Ki Paik; Tai Long Pan; Terence C.W. Poon; Ghasem Hosseini Salekdeh; Richard J. Simpson; Ravi Sirdeshmukh; Chantragan Srisomsap; Jisnuson Svasti; Yu Chang Tyan; Florian S. Dreyer; Danyl McLauchlan; Pisana Rawson; T. William Jordan

doi:10.1002/pmic.201000126

The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

Lifeng Peng, Eugene A. Kapp, David Fenyö, Min Seok Kwon, Pu Jiang, Songfeng Wu, Ying Jiang, Marie Isabel Aguilar, Nikhat Ahmed, Mark S. Baker, Zongwei CAI, Yu Ju Chen, Phan Van Chi, Maxey C.M. Chung, Fuchu He, Alice C.L. Len, Pao Chi Liao, Kazuyuki Nakamura, Sai Ming Ngai, Young Ki PaikTai Long Pan, Terence C.W. Poon, Ghasem Hosseini Salekdeh, Richard J. Simpson, Ravi Sirdeshmukh, Chantragan Srisomsap, Jisnuson Svasti, Yu Chang Tyan, Florian S. Dreyer, Danyl McLauchlan, Pisana Rawson, T. William Jordan^*

^*Corresponding author for this work

Department of Chemistry

Research output: Contribution to journal › Journal article › peer-review

16 Citations (Scopus)

Abstract

The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.

Original language	English
Pages (from-to)	4142-4148
Number of pages	7
Journal	Proteomics
Volume	10
Issue number	22
DOIs	https://doi.org/10.1002/pmic.201000126
Publication status	Published - Nov 2010

Scopus Subject Areas

Biochemistry
Molecular Biology

User-Defined Keywords

Asia Oceania Human Proteome Organisation
Cell Biology
Integral membrane proteins
Liver
Membrane proteomics

Access to Document

10.1002/pmic.201000126

Cite this

Peng, L., Kapp, E. A., Fenyö, D., Kwon, M. S., Jiang, P., Wu, S., Jiang, Y., Aguilar, M. I., Ahmed, N., Baker, M. S., CAI, Z., Chen, Y. J., Van Chi, P., Chung, M. C. M., He, F., Len, A. C. L., Liao, P. C., Nakamura, K., Ngai, S. M., ... Jordan, T. W. (2010). The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard. Proteomics, 10(22), 4142-4148. https://doi.org/10.1002/pmic.201000126

@article{dd6b545560534e7081d928a53d08998f,

title = "The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard",

abstract = "The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.",

keywords = "Asia Oceania Human Proteome Organisation, Cell Biology, Integral membrane proteins, Liver, Membrane proteomics",

author = "Lifeng Peng and Kapp, {Eugene A.} and David Feny{\"o} and Kwon, {Min Seok} and Pu Jiang and Songfeng Wu and Ying Jiang and Aguilar, {Marie Isabel} and Nikhat Ahmed and Baker, {Mark S.} and Zongwei CAI and Chen, {Yu Ju} and {Van Chi}, Phan and Chung, {Maxey C.M.} and Fuchu He and Len, {Alice C.L.} and Liao, {Pao Chi} and Kazuyuki Nakamura and Ngai, {Sai Ming} and Paik, {Young Ki} and Pan, {Tai Long} and Poon, {Terence C.W.} and {Hosseini Salekdeh}, Ghasem and Simpson, {Richard J.} and Ravi Sirdeshmukh and Chantragan Srisomsap and Jisnuson Svasti and Tyan, {Yu Chang} and Dreyer, {Florian S.} and Danyl McLauchlan and Pisana Rawson and Jordan, {T. William}",

year = "2010",

month = nov,

doi = "10.1002/pmic.201000126",

language = "English",

volume = "10",

pages = "4142--4148",

journal = "Proteomics",

issn = "1615-9853",

publisher = "Wiley-VCH Verlag",

number = "22",

}

Peng, L, Kapp, EA, Fenyö, D, Kwon, MS, Jiang, P, Wu, S, Jiang, Y, Aguilar, MI, Ahmed, N, Baker, MS, CAI, Z, Chen, YJ, Van Chi, P, Chung, MCM, He, F, Len, ACL, Liao, PC, Nakamura, K, Ngai, SM, Paik, YK, Pan, TL, Poon, TCW, Hosseini Salekdeh, G, Simpson, RJ, Sirdeshmukh, R, Srisomsap, C, Svasti, J, Tyan, YC, Dreyer, FS, McLauchlan, D, Rawson, P & Jordan, TW 2010, 'The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard', Proteomics, vol. 10, no. 22, pp. 4142-4148. https://doi.org/10.1002/pmic.201000126

TY - JOUR

T1 - The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

AU - Peng, Lifeng

AU - Kapp, Eugene A.

AU - Fenyö, David

AU - Kwon, Min Seok

AU - Jiang, Pu

AU - Wu, Songfeng

AU - Jiang, Ying

AU - Aguilar, Marie Isabel

AU - Ahmed, Nikhat

AU - Baker, Mark S.

AU - CAI, Zongwei

AU - Chen, Yu Ju

AU - Van Chi, Phan

AU - Chung, Maxey C.M.

AU - He, Fuchu

AU - Len, Alice C.L.

AU - Liao, Pao Chi

AU - Nakamura, Kazuyuki

AU - Ngai, Sai Ming

AU - Paik, Young Ki

AU - Pan, Tai Long

AU - Poon, Terence C.W.

AU - Hosseini Salekdeh, Ghasem

AU - Simpson, Richard J.

AU - Sirdeshmukh, Ravi

AU - Srisomsap, Chantragan

AU - Svasti, Jisnuson

AU - Tyan, Yu Chang

AU - Dreyer, Florian S.

AU - McLauchlan, Danyl

AU - Rawson, Pisana

AU - Jordan, T. William

PY - 2010/11

Y1 - 2010/11

N2 - The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.

AB - The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse liver that contains endoplasmic reticulum and other organelles. In this study, we present the strategy used for the preparation and initial characterization of the membrane sample, including validation that the carbonate-washing step enriches for integral and lipid-anchored membrane proteins. Analysis of 17 independent data sets from five types of proteomic workflows is in progress.

KW - Asia Oceania Human Proteome Organisation

KW - Cell Biology

KW - Integral membrane proteins

KW - Liver

KW - Membrane proteomics

UR - http://www.scopus.com/inward/record.url?scp=78449297707&partnerID=8YFLogxK

U2 - 10.1002/pmic.201000126

DO - 10.1002/pmic.201000126

M3 - Journal article

C2 - 20486120

AN - SCOPUS:78449297707

SN - 1615-9853

VL - 10

SP - 4142

EP - 4148

JO - Proteomics

JF - Proteomics

IS - 22

ER -

The Asia Oceania Human Proteome Organisation Membrane Proteomics Initiative. Preparation and characterisation of the carbonate-washed membrane standard

Abstract

Scopus Subject Areas

User-Defined Keywords

Access to Document

Other files and links

Fingerprint

Cite this