The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes

Huayan Zhao, Shiyou Lü, Ruixi Li, Tao Chen, Huoming Zhang, Peng Cui, Feng Ding, Pei Liu, Guangchao Wang, Yiji XIA, Mark P. Running, Liming XIONG*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)

Abstract

The circularly permuted GTPase large subunit GTPase 1 (LSG1) is involved in the maturation step of the 60S ribosome and is essential for cell viability in yeast. Here, an Arabidopsis mutant dig6 (drought inhibited growth of lateral roots) was isolated. The mutant exhibited multiple auxin-related phenotypes, which included reduced lateral root number, altered leaf veins, and shorter roots. Genetic mapping combined with next-generation DNA sequencing identified that the mutation occurred in AtLSG1-2. This gene was highly expressed in regions of auxin accumulation. Ribosome profiling revealed that a loss of function of AtLSG1-2 led to decreased levels of monosomes, further demonstrating its role in ribosome biogenesis. Quantitative proteomics showed that the expression of certain proteins involved in ribosome biogenesis was differentially regulated, indicating that ribosome biogenesis processes were impaired in the mutant. Further investigations showed that an AtLSG1-2 deficiency caused the alteration of auxin distribution, response, and transport in plants. It is concluded that AtLSG1-2 is integral to ribosome biogenesis, consequently affecting auxin homeostasis and plant development.

Original languageEnglish
Pages (from-to)6863-6875
Number of pages13
JournalJournal of Experimental Botany
Volume66
Issue number21
DOIs
Publication statusPublished - Nov 2015

Scopus Subject Areas

  • Physiology
  • Plant Science

User-Defined Keywords

  • Arabidopsis
  • AtLSG1
  • Auxin homeostasis
  • Expression pattern
  • Map-based cloning
  • Proteomics
  • Ribosome biogenesis

Fingerprint

Dive into the research topics of 'The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes'. Together they form a unique fingerprint.

Cite this